KEGG   ORTHOLOGY: K16954
Entry
K16954                      KO                                     

Name
mtsA
Definition
methylthiol:coenzyme M methyltransferase [EC:2.1.1.251]
Pathway
ko00920  Sulfur metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00920 Sulfur metabolism
    K16954  mtsA; methylthiol:coenzyme M methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.251  methylated-thiol---coenzyme M methyltransferase
     K16954  mtsA; methylthiol:coenzyme M methyltransferase
Other DBs
RN: R09997
GO: 0044683
Genes
MAX: MMALV_13470
MARC: AR505_1066
MBA: Mbar_A3585
MBY: MSBRM_1542
MBW: MSBRW_1148
MBAR: MSBR2_0865
MBAK: MSBR3_1223
MMA: MM_2427(mtsA)
MMAZ: MmTuc01_2485
MMJ: MSMAS_2273
MMAC: MSMAC_2181
 » show all
Reference
PMID:8932317
  Authors
Paul L, Krzycki JA
  Title
Sequence and transcript analysis of a novel Methanosarcina barkeri methyltransferase II homolog and its associated corrinoid protein homologous to methionine synthase.
  Journal
J Bacteriol 178:6599-607 (1996)
DOI:10.1128/JB.178.22.6599-6607.1996
  Sequence
Reference
  Authors
Tallant TC, Paul L, Krzycki JA
  Title
The MtsA subunit of the methylthiol:coenzyme M methyltransferase of Methanosarcina barkeri catalyses both half-reactions of corrinoid-dependent dimethylsulfide: coenzyme M methyl transfer.
  Journal
J Biol Chem 276:4485-93 (2001)
DOI:10.1074/jbc.M007514200
LinkDB

KEGG   ORTHOLOGY: K16955
Entry
K16955                      KO                                     

Name
mtsB
Definition
methylated-thiol--corrinoid protein
Pathway
ko00920  Sulfur metabolism
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00920 Sulfur metabolism
    K16955  mtsB; methylated-thiol--corrinoid protein
Other DBs
RN: R09997
Genes
MAX: MMALV_13480
MARC: AR505_1067
MBA: Mbar_A3584
MBY: MSBRM_1541 MSBRM_1543
MBW: MSBRW_1149
MBAR: MSBR2_0864 MSBR2_0866
MBAK: MSBR3_1224
MMA: MM_2428(mtsB)
MMAZ: MmTuc01_2486
MMJ: MSMAS_2272
MMAC: MSMAC_2182
 » show all
Reference
  Authors
Tallant TC, Paul L, Krzycki JA
  Title
The MtsA subunit of the methylthiol:coenzyme M methyltransferase of Methanosarcina barkeri catalyses both half-reactions of corrinoid-dependent dimethylsulfide: coenzyme M methyl transfer.
  Journal
J Biol Chem 276:4485-93 (2001)
DOI:10.1074/jbc.M007514200
LinkDB

KEGG   ENZYME: 2.1.1.251
Entry
EC 2.1.1.251                Enzyme                                 

Name
methylated-thiol---coenzyme M methyltransferase;
mtsA (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
methylated-thiol:CoM methyltransferase
Reaction(IUBMB)
methanethiol + CoM = methyl-CoM + hydrogen sulfide (overall reaction) [RN:R09997];
(1a) methanethiol + a [Co(I) methylated-thiol-specific corrinoid protein] = a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + hydrogen sulfide [RN:R10001];
(1b) a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + CoM = methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein] [RN:R10000]
Reaction(KEGG)
Substrate
methanethiol [CPD:C00409];
CoM [CPD:C03576];
[Co(I) methylated-thiol-specific corrinoid protein];
[methyl-Co(III) methylated-thiol-specific corrinoid protein]
Product
methyl-CoM [CPD:C03920];
hydrogen sulfide [CPD:C00283];
[methyl-Co(III) methylated-thiol-specific corrinoid protein];
[Co(I) methylated-thiol-specific corrinoid protein]
Comment
The enzyme, which is involved in methanogenesis from methylated thiols, such as methane thiol, dimethyl sulfide, and 3-S-methylmercaptopropionate, catalyses two successive steps - the transfer of a methyl group from the substrate to the cobalt cofactor of a methylated-thiol-specific corrinoid protein (MtsB), and the subsequent transfer of the methyl group from the corrinoid protein to CoM. With most other methanogenesis substrates this process is carried out by two different enzymes (for example, EC 2.1.1.90, methanol---corrinoid protein Co-methyltransferase, and EC 2.1.1.246, methylated methanol-specific corrinoid protein:coenzyme M methyltransferase). The cobalt is oxidized during methylation from the Co(I) state to the Co(III) state, and is reduced back to the Co(I) form during demethylation.
History
EC 2.1.1.251 created 2012
Pathway
ec00920  Sulfur metabolism
ec01100  Metabolic pathways
Orthology
K16954  methylthiol:coenzyme M methyltransferase
Genes
MAX: MMALV_13470
MARC: AR505_1066
MBA: Mbar_A3585
MBY: MSBRM_1542
MBW: MSBRW_1148
MBAR: MSBR2_0865
MBAK: MSBR3_1223
MMA: MM_2427(mtsA)
MMAZ: MmTuc01_2485
MMJ: MSMAS_2273
MMAC: MSMAC_2181
 » show all
Reference
1  [PMID:8932317]
  Authors
Paul L, Krzycki JA
  Title
Sequence and transcript analysis of a novel Methanosarcina barkeri methyltransferase II homolog and its associated corrinoid protein homologous to methionine synthase.
  Journal
J Bacteriol 178:6599-607 (1996)
DOI:10.1128/JB.178.22.6599-6607.1996
  Sequence
Reference
2  [PMID:9371433]
  Authors
Tallant TC, Krzycki JA
  Title
Methylthiol:coenzyme M methyltransferase from Methanosarcina barkeri, an enzyme of methanogenesis from dimethylsulfide and methylmercaptopropionate.
  Journal
J Bacteriol 179:6902-11 (1997)
DOI:10.1128/JB.179.22.6902-6911.1997
  Sequence
Reference
3  [PMID:11073950]
  Authors
Tallant TC, Paul L, Krzycki JA
  Title
The MtsA subunit of the methylthiol:coenzyme M methyltransferase of Methanosarcina barkeri catalyses both half-reactions of corrinoid-dependent dimethylsulfide: coenzyme M methyl transfer.
  Journal
J Biol Chem 276:4485-93 (2001)
DOI:10.1074/jbc.M007514200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.251
IUBMB Enzyme Nomenclature: 2.1.1.251
ExPASy - ENZYME nomenclature database: 2.1.1.251
BRENDA, the Enzyme Database: 2.1.1.251
LinkDB

KEGG   REACTION: R09997
Entry
R09997                      Reaction                               

Name
methanethiol:coenzyme M methyltransferase
Definition
Methanethiol + Coenzyme M <=> 2-(Methylthio)ethanesulfonate + Hydrogen sulfide
Equation
Comment
two-step reaction (see R10001+R10000)
Reaction class
RC00035  C03576_C03920
Enzyme
Pathway
rn00920  Sulfur metabolism
rn01100  Metabolic pathways
Orthology
K16954  methylthiol:coenzyme M methyltransferase [EC:2.1.1.251]
K16955  methylated-thiol--corrinoid protein
Other DBs
RHEA: 32670
LinkDB

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