KEGG   ORTHOLOGY: K18242
Entry
K18242                      KO                                     

Name
nagG
Definition
salicylate 5-hydroxylase large subunit [EC:1.14.13.172]
Pathway
ko00626  Naphthalene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Module
M00638  Salicylate degradation, salicylate => gentisate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00626 Naphthalene degradation
    K18242  nagG; salicylate 5-hydroxylase large subunit
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.172  salicylate 5-hydroxylase
     K18242  nagG; salicylate 5-hydroxylase large subunit
Other DBs
RN: R07709 R07710
COG: COG4638
Genes
EBT: EBL_c34270
PLU: plu1000
PLUM: A4R40_04995
PTT: VY86_00155
ALT: ambt_18005
TTU: TERTU_1474
MMW: Mmwyl1_2669
MME: Marme_2404
MPC: Mar181_2357
MPRI: MP3633_2253
RSO: RSc1090(nagG)
RSE: F504_1061(ohbB)
REH: H16_A1633(nagG)
CNC: CNE_1c16410(nagG)
BMK: DM80_1440
BUB: BW23_5815
BGU: KS03_3913
BFN: OI25_3046
PPNO: DA70_21370
PPNM: LV28_08950
PPUL: RO07_03180
PSPU: NA29_24090
PAPI: SG18_03370
BPT: Bpet2804
AXX: ERS451415_00957(andAc_1)
POL: Bpro_0983
PNA: Pnap_2488
AAV: Aave_0165
AJS: Ajs_3090
AAA: Acav_0196
RTA: Rta_25990
LIM: L103DPR2_01958(nagG_2)
LIH: L63ED372_02715(nagG_2)
HYB: Q5W_19155
HPSE: HPF_02890(nagG)
LCH: Lcho_0797
AZO: azo2516(nagG)
AOA: dqs_2673
SWI: Swit_3056
SPHD: HY78_10295
BLAS: BSY18_3955
TMO: TMO_a0554(bphA)
ACTI: UA75_12800
ACAD: UA74_12720
AHG: AHOG_11995(nagG)
AG: AAD12607(nagG)
 » show all
Reference
PMID:9573207
  Authors
Fuenmayor SL, Wild M, Boyes AL, Williams PA
  Title
A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2.
  Journal
J Bacteriol 180:2522-30 (1998)
DOI:10.1128/JB.180.9.2522-2530.1998
  Sequence
LinkDB

KEGG   ORTHOLOGY: K18243
Entry
K18243                      KO                                     

Name
nagH
Definition
salicylate 5-hydroxylase small subunit [EC:1.14.13.172]
Pathway
ko00626  Naphthalene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Module
M00638  Salicylate degradation, salicylate => gentisate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00626 Naphthalene degradation
    K18243  nagH; salicylate 5-hydroxylase small subunit
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.172  salicylate 5-hydroxylase
     K18243  nagH; salicylate 5-hydroxylase small subunit
Other DBs
RN: R07709 R07710
COG: COG5517
Genes
EBT: EBL_c34280
PLU: plu1001
PLUM: A4R40_05000
PTT: VY86_00150
ALT: ambt_18010
TTU: TERTU_1475
CYQ: Q91_0876 Q91_2234
CZA: CYCME_2552
CYY: CPC19_01630
MMW: Mmwyl1_2670
RSO: RSc1089(nagH)
RSN: RSPO_c02275(nagH)
RSE: F504_1060(ohbA)
REH: H16_A1632(nagH)
CNC: CNE_1c16400(nagH)
RME: Rmet_3712(nagH)
BMK: DM80_1441
BUB: BW23_5816
BGU: KS03_3914
BFN: OI25_3047
PPNO: DA70_21375
PPNM: LV28_08945
PPUL: RO07_03175
PSPU: NA29_24085
PAPI: SG18_03365
BPT: Bpet2805
AXX: ERS451415_00956(andAd_1)
POL: Bpro_0984
AAV: Aave_0166
AAA: Acav_0197
RTA: Rta_25980
LIM: L103DPR2_01957(nagH)
LIH: L63ED372_02714(nagH)
HYB: Q5W_19150
HPSE: HPF_02895(nagH)
LCH: Lcho_0796
AZO: azo2515(nagH)
AOA: dqs_2672
NAR: Saro_3834
BLAS: BSY18_3956
TMO: TMO_a0553
NFR: ERS450000_00310(andAd)
AG: AAD12608(nagH)
 » show all
Reference
PMID:9573207
  Authors
Fuenmayor SL, Wild M, Boyes AL, Williams PA
  Title
A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2.
  Journal
J Bacteriol 180:2522-30 (1998)
DOI:10.1128/JB.180.9.2522-2530.1998
  Sequence
LinkDB

KEGG   ORTHOLOGY: K14578
Entry
K14578                      KO                                     

Name
nahAb, nagAb, ndoA, nbzAb, dntAb
Definition
naphthalene 1,2-dioxygenase ferredoxin component
Pathway
ko00624  Polycyclic aromatic hydrocarbon degradation
ko00626  Naphthalene degradation
ko00627  Aminobenzoate degradation
ko00633  Nitrotoluene degradation
ko00642  Ethylbenzene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00534  Naphthalene degradation, naphthalene => salicylate
M00638  Salicylate degradation, salicylate => gentisate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00627 Aminobenzoate degradation
    K14578  nahAb, nagAb, ndoA, nbzAb, dntAb; naphthalene 1,2-dioxygenase ferredoxin component
   00633 Nitrotoluene degradation
    K14578  nahAb, nagAb, ndoA, nbzAb, dntAb; naphthalene 1,2-dioxygenase ferredoxin component
   00642 Ethylbenzene degradation
    K14578  nahAb, nagAb, ndoA, nbzAb, dntAb; naphthalene 1,2-dioxygenase ferredoxin component
   00626 Naphthalene degradation
    K14578  nahAb, nagAb, ndoA, nbzAb, dntAb; naphthalene 1,2-dioxygenase ferredoxin component
   00624 Polycyclic aromatic hydrocarbon degradation
    K14578  nahAb, nagAb, ndoA, nbzAb, dntAb; naphthalene 1,2-dioxygenase ferredoxin component
Other DBs
RN: R02968 R05422 R05423 R05424 R05425 R05426 R05427 R06909 R06930 R06937 R07704 R07706 R07709 R07710 R09159 R09233
COG: COG2146
Genes
EBT: EBL_c34290(ndoA)
PSD: DSC_14870
PPT: PPS_2607
PPI: ND054(nahAb)
PPUD: DW66_2865
PPJ: RK21_02167
PSC: A458_06730
PSTU: UIB01_22620
PBM: CL52_14605
PFK: PFAS1_28605
RMA: Rmag_0678
VOK: COSY_0630
PSE: NH8B_2003
RSO: RSc1088(nagAb)
RSL: RPSI07_2303(doxA)
RSM: CMR15_20207(doxA)
RSE: F504_1059
REH: H16_A1631(nagI)
BMK: DM80_1442(ndoA)
BUB: BW23_5817(ndoA)
BGU: KS03_3915(ndoA)
BXE: Bxe_C0940
BXB: DR64_7510(ndoA) DR64_8472
BFN: OI25_3048(ndoA)
PPNO: DA70_21380
PPNM: LV28_08940
PPUL: RO07_03170
PSPU: NA29_24080
PAPI: SG18_03360
BPAR: BN117_1348(ndoA)
BPA: BPP2189(ndoA)
BBH: BN112_1870(ndoA)
BBR: BB1586(ndoA)
BBM: BN115_1540(ndoA)
BBX: BBS798_1545(ndoA)
BPT: Bpet2806
AXX: ERS451415_00955(ndoA_1) ERS451415_02890(ndoA_2)
POL: Bpro_0985
AAV: Aave_0167
AJS: Ajs_3091
AAA: Acav_0198
CTES: O987_21885
LIM: L103DPR2_01956(ndoA)
LIH: L63ED372_02713(ndoA)
HYB: Q5W_18510
HPSE: HPF_02900(nagAb)
LCH: Lcho_0795
AZO: azo2514(nagAb)
AOA: dqs_2671
AZA: AZKH_1938
MSL: Msil_1467
JAN: Jann_3956
SHUM: STHU_47060
TMO: TMO_a0552(doxA)
NEV: NTE_01129
AG: AAA25905(nahAb) AAB09765(dntAb) AAL76201(nbzAb) AGH09225(mntAb)
 » show all
Reference
PMID:8486285
  Authors
Simon MJ, Osslund TD, Saunders R, Ensley BD, Suggs S, Harcourt A, Suen WC, Cruden DL, Gibson DT, Zylstra GJ
  Title
Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4.
  Journal
Gene 127:31-7 (1993)
DOI:10.1016/0378-1119(93)90613-8
Reference
  Authors
Dennis JJ, Zylstra GJ
  Title
Complete sequence and genetic organization of pDTG1, the 83 kilobase naphthalene degradation plasmid from Pseudomonas putida strain NCIB 9816-4.
  Journal
J Mol Biol 341:753-68 (2004)
DOI:10.1016/j.jmb.2004.06.034
  Sequence
LinkDB

KEGG   ORTHOLOGY: K14581
Entry
K14581                      KO                                     

Name
nahAa, nagAa, ndoR, nbzAa, dntAa
Definition
naphthalene 1,2-dioxygenase ferredoxin reductase component [EC:1.18.1.7]
Pathway
ko00624  Polycyclic aromatic hydrocarbon degradation
ko00626  Naphthalene degradation
ko00627  Aminobenzoate degradation
ko00633  Nitrotoluene degradation
ko00642  Ethylbenzene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00534  Naphthalene degradation, naphthalene => salicylate
M00638  Salicylate degradation, salicylate => gentisate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00627 Aminobenzoate degradation
    K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
   00633 Nitrotoluene degradation
    K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
   00642 Ethylbenzene degradation
    K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
   00626 Naphthalene degradation
    K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
   00624 Polycyclic aromatic hydrocarbon degradation
    K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.7  ferredoxin---NAD(P)+ reductase (naphthalene dioxygenase ferredoxin-specific)
     K14581  nahAa, nagAa, ndoR, nbzAa, dntAa; naphthalene 1,2-dioxygenase ferredoxin reductase component
Other DBs
RN: R02968 R05422 R05423 R05424 R05425 R05426 R05427 R06909 R06930 R06937 R07704 R07706 R07709 R07710 R09159 R09233
Genes
EBT: EBL_c34260(ndoR)
PPI: ND053(nahAa)
PSC: A458_06735
PSTU: UIB01_22615
PBM: CL52_14600
PFK: PFAS1_28600
ALT: ambt_18035
MMW: Mmwyl1_2668
MME: Marme_2403
MPC: Mar181_2356
RSO: RSc1091(nagAa)
RSL: RPSI07_2300(ndoR)
RSM: CMR15_20210(ndoR)
RSE: F504_1062
BMK: DM80_1434(ndoR) DM80_1439(ndoR)
BFN: OI25_3043(ndoR)
PPNO: DA70_21365
PPNM: LV28_08955
PPUL: RO07_03185
PSPU: NA29_24095
PAPI: SG18_03375
AFQ: AFA_02200
POL: Bpro_0980
PNA: Pnap_2489
AAV: Aave_0164
AJS: Ajs_3089
AAA: Acav_0195
LCH: Lcho_0800
TIN: Tint_1781
THI: THI_2205
AZO: azo2517(nagAa)
AOA: dqs_2674
SHZ: shn_02370
MNO: Mnod_0136
RBM: TEF_01260
PSF: PSE_0864
LABT: FIU93_04780(nagAa1)
SGI: SGRAN_2805(thnY)
TMO: TMO_2240(ndoR)
AG: AAA25904(nahAa) AAB09763(dntAa) AAD12606(nagAa) AAL76199(nbzAa) AGH09224(mntAa)
 » show all
Reference
PMID:8486285
  Authors
Simon MJ, Osslund TD, Saunders R, Ensley BD, Suggs S, Harcourt A, Suen WC, Cruden DL, Gibson DT, Zylstra GJ
  Title
Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4.
  Journal
Gene 127:31-7 (1993)
DOI:10.1016/0378-1119(93)90613-8
Reference
  Authors
Dennis JJ, Zylstra GJ
  Title
Complete sequence and genetic organization of pDTG1, the 83 kilobase naphthalene degradation plasmid from Pseudomonas putida strain NCIB 9816-4.
  Journal
J Mol Biol 341:753-68 (2004)
DOI:10.1016/j.jmb.2004.06.034
  Sequence
LinkDB

KEGG   ENZYME: 1.14.13.172
Entry
EC 1.14.13.172              Enzyme                                 

Name
salicylate 5-hydroxylase;
nagG (gene name);
nagH (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
salicylate,NADH:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
salicylate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O [RN:R07709]
Reaction(KEGG)
R07709;
(other) R07710
Substrate
salicylate [CPD:C00805];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxybenzoate [CPD:C00628];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
This enzyme, which was characterized from the bacterium Ralstonia sp. U2, comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.7, ferredoxin---NAD(P)+ reductase), an iron-sulfur oxygenase, and ferredoxin.
History
EC 1.14.13.172 created 2013
Pathway
ec00626  Naphthalene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K18242  salicylate 5-hydroxylase large subunit
K18243  salicylate 5-hydroxylase small subunit
Genes
EBT: EBL_c34270 EBL_c34280
PLU: plu1000 plu1001
PLUM: A4R40_04995 A4R40_05000
PTT: VY86_00150 VY86_00155
ALT: ambt_18005 ambt_18010
TTU: TERTU_1474 TERTU_1475
CYQ: Q91_0876 Q91_2234
CZA: CYCME_2552
CYY: CPC19_01630
MMW: Mmwyl1_2669 Mmwyl1_2670
RSO: RSc1089(nagH) RSc1090(nagG)
RSE: F504_1060(ohbA) F504_1061(ohbB)
REH: H16_A1632(nagH) H16_A1633(nagG)
CNC: CNE_1c16400(nagH) CNE_1c16410(nagG)
RME: Rmet_3712(nagH)
AXX: ERS451415_00956(andAd_1) ERS451415_00957(andAc_1)
AJS: Ajs_3090
LIM: L103DPR2_01957(nagH) L103DPR2_01958(nagG_2)
LIH: L63ED372_02714(nagH) L63ED372_02715(nagG_2)
HPSE: HPF_02890(nagG) HPF_02895(nagH)
AZO: azo2515(nagH) azo2516(nagG)
NAR: Saro_3834
TMO: TMO_a0553 TMO_a0554(bphA)
NFR: ERS450000_00310(andAd)
ACTI: UA75_12800
ACAD: UA74_12720
AHG: AHOG_11995(nagG)
 » show all
Reference
1  [PMID:9573207]
  Authors
Fuenmayor SL, Wild M, Boyes AL, Williams PA
  Title
A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2.
  Journal
J Bacteriol 180:2522-30 (1998)
DOI:10.1128/JB.180.9.2522-2530.1998
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.172
IUBMB Enzyme Nomenclature: 1.14.13.172
ExPASy - ENZYME nomenclature database: 1.14.13.172
BRENDA, the Enzyme Database: 1.14.13.172
LinkDB

KEGG   REACTION: R07709
Entry
R07709                      Reaction                               

Name
salicylate,NADH:oxygen oxidoreductase (5-hydroxylating)
Definition
Salicylate + NADH + Oxygen + H+ <=> 2,5-Dihydroxybenzoate + NAD+ + H2O
Equation
Comment
NADPH (see R07710)
Reaction class
RC00001  C00003_C00004
RC00490  C00628_C00805
Enzyme
Pathway
rn00626  Naphthalene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Module
M00638  Salicylate degradation, salicylate => gentisate
Orthology
K14578  naphthalene 1,2-dioxygenase ferredoxin component
K14581  naphthalene 1,2-dioxygenase ferredoxin reductase component [EC:1.18.1.7]
K18242  salicylate 5-hydroxylase large subunit [EC:1.14.13.172]
K18243  salicylate 5-hydroxylase small subunit [EC:1.14.13.172]
Other DBs
RHEA: 35310
LinkDB

KEGG   REACTION: R07710
Entry
R07710                      Reaction                               

Name
salicylate,NADPH:oxygen oxidoreductase (5-hydroxylating)
Definition
Salicylate + NADPH + Oxygen + H+ <=> 2,5-Dihydroxybenzoate + NADP+ + H2O
Equation
Comment
NADH (see R07709)
Reaction class
RC00001  C00005_C00006
RC00490  C00628_C00805
Enzyme
Pathway
rn00626  Naphthalene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Module
M00638  Salicylate degradation, salicylate => gentisate
Orthology
K14578  naphthalene 1,2-dioxygenase ferredoxin component
K14581  naphthalene 1,2-dioxygenase ferredoxin reductase component [EC:1.18.1.7]
K18242  salicylate 5-hydroxylase large subunit [EC:1.14.13.172]
K18243  salicylate 5-hydroxylase small subunit [EC:1.14.13.172]
LinkDB

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