KEGG   ORTHOLOGY: K20155
Entry
K20155                      KO                                     

Name
mao
Definition
4-methylaminobutanoate oxidase (methylamine-forming) [EC:1.5.3.21]
Pathway
ko00760  Nicotinate and nicotinamide metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Module
M00810  Nicotine degradation, pyridine pathway, nicotine => 2,6-dihydroxypyridine/succinate semialdehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09108 Metabolism of cofactors and vitamins
   00760 Nicotinate and nicotinamide metabolism
    K20155  mao; 4-methylaminobutanoate oxidase (methylamine-forming)
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.5  Acting on the CH-NH group of donors
   1.5.3  With oxygen as acceptor
    1.5.3.21  4-methylaminobutanoate oxidase (methylamine-forming)
     K20155  mao; 4-methylaminobutanoate oxidase (methylamine-forming)
Other DBs
RN: R10101
COG: COG1231
Genes
NCA: Noca_0585
AG: CAD47914(mao)
Reference
  Authors
Igloi GL, Brandsch R
  Title
Sequence of the 165-kilobase catabolic plasmid pAO1 from Arthrobacter nicotinovorans and identification of a pAO1-dependent nicotine uptake system.
  Journal
J Bacteriol 185:1976-86 (2003)
DOI:10.1128/JB.185.6.1976-1986.2003
  Sequence
Reference
  Authors
Chiribau CB, Mihasan M, Ganas P, Igloi GL, Artenie V, Brandsch R
  Title
Final steps in the catabolism of nicotine.
  Journal
FEBS J 273:1528-36 (2006)
DOI:10.1111/j.1742-4658.2006.05173.x
LinkDB

KEGG   ENZYME: 1.5.3.21
Entry
EC 1.5.3.21                 Enzyme                                 

Name
4-methylaminobutanoate oxidase (methylamine-forming);
mao (gene name, ambiguous)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With oxygen as acceptor
Sysname
4-methylaminobutanoate methylamidohydrolase
Reaction(IUBMB)
4-methylaminobutanoate + O2 + H2O = succinate semialdehyde + methylamine + H2O2 [RN:R10101]
Reaction(KEGG)
R10101
Substrate
4-methylaminobutanoate;
O2 [CPD:C00007];
H2O [CPD:C00001]
Product
succinate semialdehyde [CPD:C00232];
methylamine [CPD:C00218];
H2O2 [CPD:C00027]
Comment
The enzyme participates in the nicotine degradation pathway of the soil bacterium Arthrobacter nicotinovorans. Has a very weak monoamine oxidase (EC 1.4.3.4) activity with 4-aminobutanoate [1].
History
EC 1.5.3.21 created 2012
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K20155  4-methylaminobutanoate oxidase (methylamine-forming)
Genes
NCA: Noca_0585
Reference
1  [PMID:15606755]
  Authors
Chiribau CB, Sandu C, Fraaije M, Schiltz E, Brandsch R
  Title
A novel gamma-N-methylaminobutyrate demethylating oxidase involved in catabolism  of the tobacco alkaloid nicotine by Arthrobacter nicotinovorans pAO1.
  Journal
Eur J Biochem 271:4677-84 (2004)
DOI:10.1111/j.1432-1033.2004.04432.x
  Sequence
Reference
2  [PMID:16689938]
  Authors
Chiribau CB, Mihasan M, Ganas P, Igloi GL, Artenie V, Brandsch R
  Title
Final steps in the catabolism of nicotine.
  Journal
FEBS J 273:1528-36 (2006)
DOI:10.1111/j.1742-4658.2006.05173.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.5.3.21
IUBMB Enzyme Nomenclature: 1.5.3.21
ExPASy - ENZYME nomenclature database: 1.5.3.21
UM-BBD (Biocatalysis/Biodegradation Database): 1.5.3.21
BRENDA, the Enzyme Database: 1.5.3.21
LinkDB

KEGG   REACTION: R10101
Entry
R10101                      Reaction                               

Name
4-methylaminobutanoate methylamidohydrolase;
4-methylaminobutanoate:oxygen oxidoreductase (methylamine-forming)
Definition
4-Methylaminobutyrate + Oxygen + H2O <=> Succinate semialdehyde + Methylamine + Hydrogen peroxide
Equation
Reaction class
RC00225  C00232_C15987
RC00807  C00218_C15987
RC02755  C00007_C00027
Enzyme
Pathway
rn00760  Nicotinate and nicotinamide metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Module
M00810  Nicotine degradation, pyridine pathway, nicotine => 2,6-dihydroxypyridine/succinate semialdehyde
Orthology
K20155  4-methylaminobutanoate oxidase (methylamine-forming) [EC:1.5.3.21]
Other DBs
RHEA: 33918
LinkDB

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