KEGG   ORTHOLOGY: K20626
Entry
K20626                      KO                                     

Name
lcdA
Definition
lactoyl-CoA dehydratase subunit alpha [EC:4.2.1.54]
Pathway
ko00640  Propanoate metabolism
ko00643  Styrene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00640 Propanoate metabolism
    K20626  lcdA; lactoyl-CoA dehydratase subunit alpha
  09111 Xenobiotics biodegradation and metabolism
   00643 Styrene degradation
    K20626  lcdA; lactoyl-CoA dehydratase subunit alpha
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.54  lactoyl-CoA dehydratase
     K20626  lcdA; lactoyl-CoA dehydratase subunit alpha
Other DBs
RN: R02963
COG: COG1775
GO: 0018819
Genes
CNO: NT01CX_2369(hgdA)
CBN: CbC4_1683
CCT: CC1_11640
CPRO: CPRO_08380(fldB)
DOR: Desor_3092
DMI: Desmer_1800
MED: MELS_0745
MEG: DKB62_04095
FNF: BSQ88_08505
AG: AEM62994(lcdA)
Reference
  Authors
Kandasamy V, Vaidyanathan H, Djurdjevic I, Jayamani E, Ramachandran KB, Buckel W, Jayaraman G, Ramalingam S
  Title
Engineering Escherichia coli with acrylate pathway genes for propionic acid synthesis and its impact on mixed-acid fermentation.
  Journal
Appl Microbiol Biotechnol 97:1191-200 (2013)
DOI:10.1007/s00253-012-4274-y
  Sequence
LinkDB

KEGG   ORTHOLOGY: K20627
Entry
K20627                      KO                                     

Name
lcdB
Definition
lactoyl-CoA dehydratase subunit beta [EC:4.2.1.54]
Pathway
ko00640  Propanoate metabolism
ko00643  Styrene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00640 Propanoate metabolism
    K20627  lcdB; lactoyl-CoA dehydratase subunit beta
  09111 Xenobiotics biodegradation and metabolism
   00643 Styrene degradation
    K20627  lcdB; lactoyl-CoA dehydratase subunit beta
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.54  lactoyl-CoA dehydratase
     K20627  lcdB; lactoyl-CoA dehydratase subunit beta
Other DBs
RN: R02963
COG: COG1775
GO: 0018819
Genes
CNO: NT01CX_2368(hgdB)
CBN: CbC4_1682
CCT: CC1_11650
CPRO: CPRO_08390(fldC)
DOR: Desor_3093
DMI: Desmer_1801
MED: MELS_0746
MEG: DKB62_04100
FNF: BSQ88_08500
AG: AEM62993(lcdB)
Reference
  Authors
Kandasamy V, Vaidyanathan H, Djurdjevic I, Jayamani E, Ramachandran KB, Buckel W, Jayaraman G, Ramalingam S
  Title
Engineering Escherichia coli with acrylate pathway genes for propionic acid synthesis and its impact on mixed-acid fermentation.
  Journal
Appl Microbiol Biotechnol 97:1191-200 (2013)
DOI:10.1007/s00253-012-4274-y
  Sequence
LinkDB

KEGG   ENZYME: 4.2.1.54
Entry
EC 4.2.1.54                 Enzyme                                 

Name
lactoyl-CoA dehydratase;
lactoyl coenzyme A dehydratase;
lactyl-coenzyme A dehydrase;
lactyl CoA dehydratase;
acrylyl coenzyme A hydratase;
lactoyl-CoA hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
(R)-lactoyl-CoA hydro-lyase (acryloyl-CoA-forming)
Reaction(IUBMB)
(R)-lactoyl-CoA = acryloyl-CoA + H2O [RN:R02963]
Reaction(KEGG)
R02963
Substrate
(R)-lactoyl-CoA
Product
acryloyl-CoA [CPD:C00894];
H2O [CPD:C00001]
Comment
A bacterial enzyme that is involved in propanoate fermentation (also known as the acrylate pathway).
History
EC 4.2.1.54 created 1972, modified 2012
Pathway
ec00640  Propanoate metabolism
ec00643  Styrene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K20626  lactoyl-CoA dehydratase subunit alpha
K20627  lactoyl-CoA dehydratase subunit beta
Genes
CNO: NT01CX_2368(hgdB) NT01CX_2369(hgdA)
CBN: CbC4_1682 CbC4_1683
CCT: CC1_11640 CC1_11650
CPRO: CPRO_08380(fldB) CPRO_08390(fldC)
DOR: Desor_3092 Desor_3093
DMI: Desmer_1800 Desmer_1801
MED: MELS_0745 MELS_0746
MEG: DKB62_04095 DKB62_04100
FNF: BSQ88_08500 BSQ88_08505
Reference
1  [PMID:14292829]
  Authors
BALDWIN RL, WOOD WA, EMERY RS.
  Title
LACTATE METABOLISM BY PEPTOSTREPTOCOCCUS ELSDENII: EVIDENCE FOR LACTYL COENZYME A DEHYDRASE.
  Journal
Biochim Biophys Acta 97:202-13 (1965)
DOI:10.1016/0304-4165(65)90084-X
Reference
2  [PMID:6586495]
  Authors
Schweiger G, Buckel W
  Title
On the dehydration of (R)-lactate in the fermentation of alanine to propionate by Clostridium propionicum.
  Journal
FEBS Lett 171:79-84 (1984)
DOI:10.1016/0014-5793(84)80463-9
Reference
3  [PMID:4055736]
  Authors
Kuchta RD, Abeles RH
  Title
Lactate reduction in Clostridium propionicum. Purification and properties of lactyl-CoA dehydratase.
  Journal
J Biol Chem 260:13181-9 (1985)
  Sequence
Reference
4  [PMID:3026450]
  Authors
Kuchta RD, Hanson GR, Holmquist B, Abeles RH
  Title
Fe-S centers in lactyl-CoA dehydratase.
  Journal
Biochemistry 25:7301-7 (1986)
DOI:10.1021/bi00371a009
Reference
5  [PMID:1597194]
  Authors
Hofmeister AE, Buckel W
  Title
(R)-lactyl-CoA dehydratase from Clostridium propionicum. Stereochemistry of the dehydration of (R)-2-hydroxybutyryl-CoA to crotonyl-CoA.
  Journal
Eur J Biochem 206:547-52 (1992)
DOI:10.1111/j.1432-1033.1992.tb16958.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.54
IUBMB Enzyme Nomenclature: 4.2.1.54
ExPASy - ENZYME nomenclature database: 4.2.1.54
UM-BBD (Biocatalysis/Biodegradation Database): 4.2.1.54
BRENDA, the Enzyme Database: 4.2.1.54
CAS: 9031-12-3
LinkDB

KEGG   REACTION: R02963
Entry
R02963                      Reaction                               

Name
lactoyl-CoA hydro-lyase
Definition
Lactoyl-CoA <=> Propenoyl-CoA + H2O
Equation
Reaction class
RC00818  C00827_C00894
Enzyme
Pathway
rn00640  Propanoate metabolism
rn00643  Styrene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K20626  lactoyl-CoA dehydratase subunit alpha [EC:4.2.1.54]
K20627  lactoyl-CoA dehydratase subunit beta [EC:4.2.1.54]
Other DBs
RHEA: 21059
LinkDB

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