KEGG   ORTHOLOGY: K21273
Entry
K21273                      KO                                     

Name
E2.5.1.88
Definition
trans,polycis-polyprenyl diphosphate synthase [EC:2.5.1.88]
Pathway
ko00900  Terpenoid backbone biosynthesis
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00900 Terpenoid backbone biosynthesis
    K21273  E2.5.1.88; trans,polycis-polyprenyl diphosphate synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.88  trans,polycis-polyprenyl diphosphate synthase [(2Z,6E)-farnesyl diphosphate specific]
     K21273  E2.5.1.88; trans,polycis-polyprenyl diphosphate synthase
Other DBs
RN: R09731
COG: COG0020
Genes
PHW: G7075_08440
BLUT: EW640_02245
PAUS: NCTC13651_01488(uppS)
PRV: G7070_00365
NSN: EXE58_14895
KQI: F1D05_32195
TFU: Tfu_0853
NDA: Ndas_0730
NGV: CDO52_20120
STRR: EKD16_07660
TCU: Tcur_3231
SRO: Sros_2336
SAQ: Sare_3825
MIL: ML5_3461
ASE: ACPL_1421
ACTN: L083_1597
AFS: AFR_08000
ACTS: ACWT_1300
PFLA: Pflav_057510(uppS1)
PSUU: Psuf_017140(uppS1)
SNA: Snas_2041
TBI: Tbis_1177
 » show all
Reference
  Authors
Ambo T, Noike M, Kurokawa H, Koyama T
  Title
Cloning and functional analysis of cis-prenyltransferase from Thermobifida fusca.
  Journal
J Biosci Bioeng 107:620-2 (2009)
DOI:10.1016/j.jbiosc.2009.02.006
  Sequence
[tfu:Tfu_0853]
LinkDB

KEGG   ENZYME: 2.5.1.88
Entry
EC 2.5.1.88                 Enzyme                                 

Name
trans,polycis-polyprenyl diphosphate synthase [(2Z,6E)-farnesyl diphosphate specific]
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
(2Z,6E)-farnesyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 9--11 isopentenyl units)
Reaction(IUBMB)
(2Z,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + trans,polycis-polyprenyl diphosphate (n = 9--11) [RN:R09731]
Reaction(KEGG)
R09731
Substrate
(2Z,6E)-farnesyl diphosphate [CPD:C16826];
isopentenyl diphosphate [CPD:C00129]
Product
diphosphate [CPD:C00013];
trans,polycis-polyprenyl diphosphate [CPD:C19853]
Comment
Highest activity with (2Z,6E)-farnesyl diphosphate as allylic substrate. Broad product specificity with the major product being dodecaprenyl diphosphate. Synthesizes even C70 prenyl diphosphate as the maximum chain-length product [1].
History
EC 2.5.1.88 created 2010
Pathway
ec00900  Terpenoid backbone biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K21273  trans,polycis-polyprenyl diphosphate synthase
Genes
PHW: G7075_08440
BLUT: EW640_02245
PAUS: NCTC13651_01488(uppS)
PRV: G7070_00365
NSN: EXE58_14895
KQI: F1D05_32195
TFU: Tfu_0853
NDA: Ndas_0730
NGV: CDO52_20120
STRR: EKD16_07660
TCU: Tcur_3231
SRO: Sros_2336
SAQ: Sare_3825
MIL: ML5_3461
ASE: ACPL_1421
ACTN: L083_1597
AFS: AFR_08000
ACTS: ACWT_1300
PFLA: Pflav_057510(uppS1)
PSUU: Psuf_017140(uppS1)
SNA: Snas_2041
TBI: Tbis_1177
 » show all
Reference
1  [PMID:19447338]
  Authors
Ambo T, Noike M, Kurokawa H, Koyama T
  Title
Cloning and functional analysis of cis-prenyltransferase from Thermobifida fusca.
  Journal
J Biosci Bioeng 107:620-2 (2009)
DOI:10.1016/j.jbiosc.2009.02.006
  Sequence
[tfu:Tfu_0853]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.88
IUBMB Enzyme Nomenclature: 2.5.1.88
ExPASy - ENZYME nomenclature database: 2.5.1.88
BRENDA, the Enzyme Database: 2.5.1.88
LinkDB

KEGG   REACTION: R09731
Entry
R09731                      Reaction                               

Name
(2Z,6E)-farnesyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 9-11 isopentenyl units)
Definition
2-cis,6-trans-Farnesyl diphosphate + n Isopentenyl diphosphate <=> n Diphosphate + trans,polycis-Polyprenyl diphosphate
Equation
C16826 + n C00129 <=> n C00013 + C19853
Reaction class
RC00279  C00129_C19853
RC02839  C16826_C19853
Enzyme
Pathway
rn00900  Terpenoid backbone biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K21273  trans,polycis-polyprenyl diphosphate synthase [EC:2.5.1.88]
LinkDB

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