KEGG   ORTHOLOGY: K21683Help
Entry
K21683                      KO                                     

Name
dntB
Definition
4-methyl-5-nitrocatechol 5-monooxygenase [EC:1.14.13.210]
Pathway
ko00633  Nitrotoluene degradation
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00633 Nitrotoluene degradation
    K21683  dntB; 4-methyl-5-nitrocatechol 5-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.210  4-methyl-5-nitrocatechol 5-monooxygenase
     K21683  dntB; 4-methyl-5-nitrocatechol 5-monooxygenase
BRITE hierarchy
Other DBs
RN: R09234 R11150
COG: COG0654
Genes
AG: AAC44479(dntB) ABC00744(dntB)
TaxonomyKoalaUniProt
Reference
PMID:8830701
  Authors
Haigler BE, Suen WC, Spain JC
  Title
Purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from Burkholderia sp. strain DNT.
  Journal
J Bacteriol 178:6019-24 (1996)
DOI:10.1128/JB.178.20.6019-6024.1996
  Sequence
Reference
  Authors
Leungsakul T, Johnson GR, Wood TK
  Title
Protein engineering of the 4-methyl-5-nitrocatechol monooxygenase from Burkholderia sp. strain DNT for enhanced degradation of nitroaromatics.
  Journal
Appl Environ Microbiol 72:3933-9 (2006)
DOI:10.1128/AEM.02966-05
  Sequence
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KEGG   ENZYME: 1.14.13.210Help
Entry
EC 1.14.13.210              Enzyme                                 

Name
4-methyl-5-nitrocatechol 5-monooxygenase;
dntB (gene name);
4-methyl-5-nitrocatechol oxygenase;
MNC monooxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
4-methyl-5-nitrocatechol,NAD(P)H:oxygen 5-oxidoreductase (5-hydroxylating, nitrite-forming)
Reaction(IUBMB)
4-methyl-5-nitrocatechol + NAD(P)H + H+ + O2 = 2-hydroxy-5-methylquinone + nitrite + NAD(P)+ + H2O [RN:R09234 R11150]
Reaction(KEGG)
Substrate
4-methyl-5-nitrocatechol [CPD:C18315];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2-hydroxy-5-methylquinone [CPD:C18316];
nitrite [CPD:C00088];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Contains FAD. The enzyme, isolated from the bacterium Burkholderia sp. DNT, can use both NADH and NADPH, but prefers NADPH. It has a narrow substrate range, but can also act on 4-nitrocatechol.
History
EC 1.14.13.210 created 2016
Pathway
ec00633  Nitrotoluene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K21683  4-methyl-5-nitrocatechol 5-monooxygenase
Reference
1  [PMID:8830701]
  Authors
Haigler BE, Suen WC, Spain JC.
  Title
Purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from Burkholderia sp. strain DNT.
  Journal
J Bacteriol 178:6019-24 (1996)
DOI:10.1128/JB.178.20.6019-6024.1996
  Sequence
Reference
2  [PMID:16751499]
  Authors
Leungsakul T, Johnson GR, Wood TK.
  Title
Protein engineering of the 4-methyl-5-nitrocatechol monooxygenase from Burkholderia sp. strain DNT for enhanced degradation of nitroaromatics.
  Journal
Appl Environ Microbiol 72:3933-9 (2006)
DOI:10.1128/AEM.02966-05
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.210
IUBMB Enzyme Nomenclature: 1.14.13.210
ExPASy - ENZYME nomenclature database: 1.14.13.210
BRENDA, the Enzyme Database: 1.14.13.210
LinkDB All DBs

KEGG   REACTION: R09234Help
Entry
R09234                      Reaction                               

Name
4-methyl-5-nitrocatechol,NADPH:oxygen 5-oxidoreductase (5-hydroxylating, nitrite-forming)
Definition
4-Methyl-5-nitrocatechol + NADPH + Oxygen + H+ <=> 2-Hydroxy-5-methylquinone + NADP+ + Nitrite + H2O
Equation
Comment
NADH (see R11150)
Reaction class
RC00001  C00005_C00006
RC03020  C18315_C18316
Enzyme
Pathway
rn00633  Nitrotoluene degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K21683  4-methyl-5-nitrocatechol 5-monooxygenase [EC:1.14.13.210]
Other DBs
RHEA: 48015
LinkDB All DBs

KEGG   REACTION: R11150Help
Entry
R11150                      Reaction                               

Name
4-methyl-5-nitrocatechol,NADH:oxygen 5-oxidoreductase (5-hydroxylating, nitrite-forming)
Definition
4-Methyl-5-nitrocatechol + NADH + Oxygen + H+ <=> 2-Hydroxy-5-methylquinone + NAD+ + Nitrite + H2O
Equation
Comment
NADPH (see R09234)
Reaction class
RC00001  C00003_C00004
RC03020  C18315_C18316
Enzyme
Pathway
rn00633  Nitrotoluene degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K21683  4-methyl-5-nitrocatechol 5-monooxygenase [EC:1.14.13.210]
LinkDB All DBs

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