KEGG   ORTHOLOGY: K23109
Entry
K23109                      KO                                     

Name
fldH
Definition
aromatic 2-oxoacid reductase [EC:1.1.1.110]
Pathway
ko00350  Tyrosine metabolism
ko00360  Phenylalanine metabolism
ko00380  Tryptophan metabolism
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00350 Tyrosine metabolism
    K23109  fldH; aromatic 2-oxoacid reductase
   00360 Phenylalanine metabolism
    K23109  fldH; aromatic 2-oxoacid reductase
   00380 Tryptophan metabolism
    K23109  fldH; aromatic 2-oxoacid reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.110  aromatic 2-oxoacid reductase
     K23109  fldH; aromatic 2-oxoacid reductase
Other DBs
RN: R01370 R03337 R12256
COG: COG1052
GO: 0097256
Genes
CBO: CBO3284(fldH)
CBA: CLB_3340(ldhA-2)
CBH: CLC_3226(ldhA-2)
CBY: CLM_3717(ldhA)
CBL: CLK_2700
CBB: CLD_1239
CBI: CLJ_B3564
CBF: CLI_3454(ldhA-2)
CBM: CBF_3436(ldhA-2)
CBJ: H04402_03369
CLD: CLSPO_c34040(fldH)
 » show all
Reference
  Authors
Dickert S, Pierik AJ, Linder D, Buckel W
  Title
The involvement of coenzyme A esters in the dehydration of (R)-phenyllactate to (E)-cinnamate by Clostridium sporogenes.
  Journal
Eur J Biochem 267:3874-84 (2000)
DOI:10.1046/j.1432-1327.2000.01427.x
  Sequence
Reference
  Authors
Dodd D, Spitzer MH, Van Treuren W, Merrill BD, Hryckowian AJ, Higginbottom SK, Le A, Cowan TM, Nolan GP, Fischbach MA, Sonnenburg JL
  Title
A gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites.
  Journal
Nature 551:648-652 (2017)
DOI:10.1038/nature24661
  Sequence
LinkDB

KEGG   ENZYME: 1.1.1.110
Entry
EC 1.1.1.110                Enzyme                                 

Name
aromatic 2-oxoacid reductase;
(R)-aromatic lactate dehydrogenase;
(R)-4-hydroxyphenyllactate dehydrogenase;
indolelactate:NAD+ oxidoreductase;
indolelactate dehydrogenase;
fldH (gene name);
(indol-3-yl)lactate:NAD+ oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
aromatic 2-oxoacid:NAD+ oxidoreductase
Reaction(IUBMB)
(1) (R)-3-(phenyl)lactate + NAD+ = 3-phenylpyruvate + NADH + H+ [RN:R01370];
(2) (R)-3-(4-hydroxyphenyl)lactate + NAD+ = 3-(4-hydroxyphenyl)pyruvate + NADH + H+ [RN:R03337];
(3) (R)-(indol-3-yl)lactate + NAD+ = (indol-3-yl)pyruvate + NADH + H+ [RN:R12256]
Reaction(KEGG)
Substrate
(R)-3-(phenyl)lactate [CPD:C05607];
NAD+ [CPD:C00003];
(R)-3-(4-hydroxyphenyl)lactate [CPD:C03964];
(R)-(indol-3-yl)lactate [CPD:C22006]
Product
3-phenylpyruvate;
NADH [CPD:C00004];
H+ [CPD:C00080];
3-(4-hydroxyphenyl)pyruvate [CPD:C01179];
(indol-3-yl)pyruvate [CPD:C00331]
Comment
The enzymes from anaerobic bacteria such as Clostridium sporogenes participate in the fermentation pathways of L-phenylalanine, L-tyrosine and L-tryptophan. The enzyme from the yeast Candida maltosa has similar activity, but, unlike the bacterial enzyme, requires Mn2+ and can also use NADPH with lower activity.
History
EC 1.1.1.110 created 1972 (EC 1.1.1.222 created 2000, incorporated 2018), modified 2018
Pathway
ec00350  Tyrosine metabolism
ec00360  Phenylalanine metabolism
ec00380  Tryptophan metabolism
ec00960  Tropane, piperidine and pyridine alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K23109  aromatic 2-oxoacid reductase
Genes
CBO: CBO3284(fldH)
CBA: CLB_3340(ldhA-2)
CBH: CLC_3226(ldhA-2)
CBY: CLM_3717(ldhA)
CBL: CLK_2700
CBB: CLD_1239
CBI: CLJ_B3564
CBF: CLI_3454(ldhA-2)
CBM: CBF_3436(ldhA-2)
CBJ: H04402_03369
CLD: CLSPO_c34040(fldH)
 » show all
Reference
1  [PMID:4384683]
  Authors
Jean M, DeMoss RD.
  Title
Indolelactate dehydrogenase from Clostridium sporogenes.
  Journal
Can J Microbiol 14:429-35 (1968)
DOI:10.1139/m68-068
Reference
2  [PMID:6354130]
  Authors
Giesel H, Simon H
  Title
On the occurrence of enoate reductase and 2-oxo-carboxylate reductase in clostridia and some observations on the amino acid fermentation by Peptostreptococcus anaerobius.
  Journal
Arch Microbiol 135:51-7 (1983)
DOI:10.1007/bf00419482
Reference
3
  Authors
Bode, R., Lippoldt, A. and Birnbaum, D.
  Title
Purification and properties of D-aromatic lactate dehydrogenase an enzyme involved in the catabolism of the aromatic amino acids of Candida maltosa.
  Journal
Biochem Physiol Pflanzen 181:189-198 (1986)
Reference
4  [PMID:10849007]
  Authors
Dickert S, Pierik AJ, Linder D, Buckel W
  Title
The involvement of coenzyme A esters in the dehydration of (R)-phenyllactate to (E)-cinnamate by Clostridium sporogenes.
  Journal
Eur J Biochem 267:3874-84 (2000)
DOI:10.1046/j.1432-1327.2000.01427.x
  Sequence
Reference
5  [PMID:29168502]
  Authors
Dodd D, Spitzer MH, Van Treuren W, Merrill BD, Hryckowian AJ, Higginbottom SK, Le A, Cowan TM, Nolan GP, Fischbach MA, Sonnenburg JL
  Title
A gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites.
  Journal
Nature 551:648-652 (2017)
DOI:10.1038/nature24661
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.110
IUBMB Enzyme Nomenclature: 1.1.1.110
ExPASy - ENZYME nomenclature database: 1.1.1.110
BRENDA, the Enzyme Database: 1.1.1.110
CAS: 37250-41-2
LinkDB

KEGG   REACTION: R03337
Entry
R03337                      Reaction                               

Name
(R)-3-(4-hydroxyphenyl)lactate:NAD+ 2-oxidoreductase
Definition
(R)-3-(4-Hydroxyphenyl)lactate + NAD+ <=> 3-(4-Hydroxyphenyl)pyruvate + NADH + H+
Equation
Comment
NADP+ (see R03339)
Reaction class
RC00001  C00003_C00004
RC00031  C01179_C03964
Enzyme
1.1.1.110       1.1.1.237
Pathway
rn00130  Ubiquinone and other terpenoid-quinone biosynthesis
rn00350  Tyrosine metabolism
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K13574  hydroxycarboxylate dehydrogenase B [EC:1.1.1.237 1.1.1.-]
K18606  hydroxyphenylpyruvate reductase [EC:1.1.1.237]
K23109  aromatic 2-oxoacid reductase [EC:1.1.1.110]
Other DBs
RHEA: 10783
LinkDB

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