KEGG   ENZYME: 1.1.1.203
Entry
EC 1.1.1.203                Enzyme                                 

Name
uronate dehydrogenase;
uronate:NAD-oxidoreductase;
uronic acid dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
uronate:NAD+ 1-oxidoreductase
Reaction(IUBMB)
(1) beta-D-galacturonate + NAD+ = D-galactaro-1,5-lactone + NADH + H+ [RN:R10841];
(2) beta-D-glucuronate + NAD+ = D-glucaro-1,5-lactone + NADH + H+ [RN:R10842]
Reaction(KEGG)
R10841 R10842
Substrate
beta-D-galacturonate;
NAD+ [CPD:C00003];
beta-D-glucuronate
Product
D-galactaro-1,5-lactone [CPD:C20889];
NADH [CPD:C00004];
H+ [CPD:C00080];
D-glucaro-1,5-lactone [CPD:C20890]
Comment
Requires Mg2+. The enzyme, characterized from the bacterium Agrobacterium fabrum, participates in oxidative degradation pathways for galacturonate and glucuronate. The enzyme can only accept the beta anomeric form of the substrate [4]. The 1,5-lactone product is rather stable at cytosolic pH and does not hydrolyse spontaneously at a substantial rate.
History
EC 1.1.1.203 created 1972 as EC 1.2.1.35, transferred 1984 to EC 1.1.1.203, modified 2014
Pathway
ec00053  Ascorbate and aldarate metabolism
ec01100  Metabolic pathways
Orthology
K18981  uronate dehydrogenase
Genes
EPE: CI789_10375
PMY: Pmen_3299
PMK: MDS_3626
PRE: PCA10_06290(udh)
PPU: PP_1171(udh)
PPF: Pput_1205 Pput_1875
PPG: PputGB1_4240
PPW: PputW619_1189
PPB: PPUBIRD1_1216 PPUBIRD1_1845
PPI: YSA_07420 YSA_08930
PPUN: PP4_11170(udh) PP4_20650(udh)
PSB: Psyr_0902
PSYR: N018_21145
PFL: PFL_4587
PPRO: PPC_4689
PFB: VO64_1030
PMAN: OU5_6132
PSTT: CH92_11110
PSOS: POS17_4654
PANR: A7J50_3606
PSIL: PMA3_15595
PALL: UYA_17350
CSA: Csal_2474
HEL: HELO_3405(udh)
HAM: HALO3641
SALN: SALB1_0476
RSO: RSp0831
RSN: RSPO_m01149(wcaG)
RSE: F504_4388
RSY: RSUY_40160(udh_1)
RPI: Rpic_4447
BUT: X994_6509
BTE: BTH_I0191
BTQ: BTQ_216
BTJ: BTJ_2269
BTZ: BTL_184
BTD: BTI_3529
BTV: BTHA_241
BTHE: BTN_1208
BTHM: BTRA_385
BTHA: DR62_1383
BTHL: BG87_348
BOK: DM82_3415
BOC: BG90_1403
BVE: AK36_4725
BCJ: BCAM0486
BCEN: DM39_4655
BCEW: DM40_3591
BCEO: I35_4379
BAM: Bamb_5236
BMU: Bmul_5164
BMK: DM80_4342
BMUL: NP80_3554
BCT: GEM_5230
BCED: DM42_4664
BDL: AK34_4888
BCON: NL30_23445
BUB: BW23_4239
BLAT: WK25_20560
BTEI: WS51_01235
BSEM: WJ12_22430
BPSL: WS57_11350
BMEC: WJ16_21460
BSTG: WT74_21665
BGU: KS03_1928
BUK: MYA_3657
BUL: BW21_15
PPNO: DA70_18130
PPNM: LV28_12655
PPUL: RO07_06635
PSPU: NA29_02505
PAPI: SG18_07025
CABA: SBC2_19510(udh_1) SBC2_62080(udh_2)
AXX: ERS451415_05379(rmlB)
POL: Bpro_3109
PNA: Pnap_1591
AAV: Aave_2590
AAA: Acav_2613
VEI: Veis_1238
HPSE: HPF_05190(rmlB)
JAG: GJA_1432
HSE: Hsero_4608(wcaG)
HRB: Hrubri_4545(wcaG)
CFU: CFU_0232
CARE: LT85_0823
LCH: Lcho_1115
PBH: AAW51_4399(udh)
SME: SMc04145
SMER: DU99_00575
SMD: Smed_3319
ARA: Arad_9474
AVI: Avi_5694
RLE: pRL120778
RLG: Rleg_5176
SHZ: shn_22915
OAN: Oant_3405
OAH: DR92_3082
OCH: CES85_2646(udh)
BJA: blr2818
BRA: BRADO2488
BBT: BBta_2833
BRS: S23_51790
AOL: S58_51270
BRAD: BF49_1864
XAU: Xaut_3373
MET: M446_0612
MOR: MOC_3922
MAQU: Maq22A_c26490(wcaG)
MMED: Mame_00555(gerKI) Mame_00565
CAK: Caul_1797
CSE: Cseg_1794
JAN: Jann_3497
RDE: RD1_2062
KVL: KVU_1858
KVU: EIO_2319
OTM: OSB_27000
RHC: RGUI_3939
RID: RIdsm_01832(rmlB)
SHUM: STHU_47620
TXI: TH3_13580
BWW: bwei_1507
BMYO: BG05_2577
PPM: PPSC2_17220(agmE)
PPO: PPM_3475(agmE)
PPOY: RE92_19875
PMW: B2K_01220
PRI: PRIO_1815
JEO: JMA_33520
CRN: CAR_c18080(ytbQ)
ROP: ROP_57580
RFA: A3L23_01449(udh)
RHS: A3Q41_01908(udh)
SCO: SCO1896(SCI7.14)
SGR: SGR_5616
SGB: WQO_07265
SCT: SCAT_1075
SFA: Sfla_4918
SHY: SHJG_3347
SVE: SVEN_1530
STRP: F750_1764
SFI: SFUL_2918
SALU: DC74_2381
SALL: SAZ_13260
STRE: GZL_06626
SLD: T261_6270
SPRI: SPRI_5628
SLE: sle_52380(sle_52380)
STRD: NI25_29720
SMAL: SMALA_1848
SLAU: SLA_0609
SALJ: SMD11_5126
SFK: KY5_1806
SGE: DWG14_06501(udh)
SRJ: SRO_7082
CMC: CMN_00338
ART: Arth_0483
ACH: Achl_2093
NCA: Noca_3523
KFL: Kfla_4972
TFU: Tfu_1140
FAL: FRAAL2584
GOB: Gobs_4379
MMAR: MODMU_4786
AOI: AORI_5004
AMYY: YIM_14765
AORI: SD37_24640
PSEH: XF36_20020
PAUT: Pdca_11890
AMI: Amir_1485
KAL: KALB_6873
ACTI: UA75_23085
ACAD: UA74_22565
ASE: ACPL_3607
AFS: AFR_26140
ACTS: ACWT_3479
SNA: Snas_0760
TBI: Tbis_1085
DRA: DR_A0222
DGE: Dgeo_2417
DFC: DFI_17465
TTR: Tter_1729
FTJ: FTUN_6508
 » show all
Reference
1  [PMID:13657147]
  Authors
KILGORE WW, STARR MP.
  Title
Uronate oxidation by phytopathogenic pseudomonads.
  Journal
Nature 183:1412-3 (1959)
DOI:10.1038/1831412a0
Reference
2  [PMID:19921179]
  Authors
Boer H, Maaheimo H, Koivula A, Penttila M, Richard P
  Title
Identification in Agrobacterium tumefaciens of the D-galacturonic acid dehydrogenase gene.
  Journal
Appl Microbiol Biotechnol 86:901-9 (2010)
DOI:10.1007/s00253-009-2333-9
  Sequence
[atu:Atu3143]
Reference
3  [PMID:22493433]
  Authors
Andberg M, Maaheimo H, Boer H, Penttila M, Koivula A, Richard P
  Title
Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate.
  Journal
J Biol Chem 287:17662-71 (2012)
DOI:10.1074/jbc.M111.335240
Reference
4  [PMID:21676870]
  Authors
Parkkinen T, Boer H, Janis J, Andberg M, Penttila M, Koivula A, Rouvinen J
  Title
Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens.
  Journal
J Biol Chem 286:27294-300 (2011)
DOI:10.1074/jbc.M111.254854
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.203
IUBMB Enzyme Nomenclature: 1.1.1.203
ExPASy - ENZYME nomenclature database: 1.1.1.203
BRENDA, the Enzyme Database: 1.1.1.203
CAS: 37250-98-9
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