KEGG   ENZYME: 1.1.1.304
Entry
EC 1.1.1.304                Enzyme                                 

Name
diacetyl reductase [(S)-acetoin forming];
(S)-acetoin dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
(S)-acetoin:NAD+ oxidoreductase
Reaction(IUBMB)
(S)-acetoin + NAD+ = diacetyl + NADH + H+ [RN:R09078]
Reaction(KEGG)
R09078
Substrate
(S)-acetoin [CPD:C01769];
NAD+ [CPD:C00003]
Product
diacetyl [CPD:C00741];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
The reaction is catalysed in the reverse direction. This activity is usually associated with butanediol dehydrogenase activity (EC 1.1.1.4 or EC 1.1.1.76). While the butanediol dehydrogenase activity is reversible, diacetyl reductase activity is irreversible. This enzyme has been reported in the bacteria Geobacillus stearothermophilus, Enterobacter aerogenes and Klebsiella pneumoniae [1-3]. Different from EC 1.1.1.303, diacetyl reductase [(R)-acetoin forming].
History
EC 1.1.1.304 created 2010 (EC 1.1.1.5 created 1961, modified 1976, part incorporated 2010)
Pathway
ec00650  Butanoate metabolism
Orthology
K03366  meso-butanediol dehydrogenase / (S,S)-butanediol dehydrogenase / diacetyl reductase
K18009  meso-butanediol dehydrogenase / (S,S)-butanediol dehydrogenase / diacetyl reductase
Genes
LVE: 103080004 103089732
DSR: 110180942
LCQ: 111680290 111681784
EUN: UMNK88_pK8827
ENA: ECNA114_0515
ECOS: EC958_0692
ECY: ECSE_P3-0048
ESE: ECSF_0514
ECOJ: P423_02810
ENC: ECL_03125
ECLX: LI66_05535
ECLY: LI62_06440
ECLZ: LI64_06070
ECLO: ENC_21670
EEC: EcWSU1_01150(budC)
ENF: AKI40_0780(budC)
CSK: ES15_1123(butA) ES15_2024
CTU: CTU_21210 CTU_29850(budC)
KPN: KPN_02061
KPT: VK055_0406(budC)
KPR: KPR_3097(budC) KPR_3966
KPX: PMK1_02920(fabG_6) PMK1_04423(budC)
KPNK: BN49_3168(budC)
KPE: KPK_2269(budC) KPK_3986
CAMA: F384_02520
RTG: NCTC13098_01134(budC_1) NCTC13098_03196(budC_2) NCTC13098_05459(fabG_15)
REE: electrica_00804(budC_1) electrica_02167(budC_2)
CLAP: NCTC11466_02427(budC)
KIE: NCTC12125_02727(budC)
AHN: NCTC12129_03729(fabG_7)
PSTS: E05_23120(yxbG)
YRU: BD65_651
SMAR: SM39_1526
SPE: Spro_2063
SRL: SOD_c04890(moaE1) SOD_c18750(yxbG) SOD_c30770(butA)
SPLY: Q5A_010375(cpnA) Q5A_016695(budC)
SERF: L085_18355
SOF: NCTC11214_04763(cpnA)
RAA: Q7S_23296
ECA: ECA0310(budC)
PATR: EV46_01685
PATO: GZ59_03420(budC)
PCT: PC1_0296
PEC: W5S_0317
DDQ: DDI_3151
DAQ: DAQ1742_00942(budC)
PAM: PANA_1671(fabG) PANA_1749(yxbG) PANA_3970(budC)
PAJ: PAJ_1021(fabG) PAJ_1094(yxbG) PAJ_p0039(budC)
MINT: C7M51_01919(fabG2) C7M51_02488 C7M51_03459(budC_2)
MTHI: C7M52_00365 C7M52_00883(fabG_3) C7M52_03590(budC)
PMR: PMI2092(budC)
PMIB: BB2000_2165(budC)
PHAU: PH4a_02420
XBO: XBJ1_2718
XBV: XBW1_2585
XDO: XDD1_2324 XDD1_3345(butA)
XPO: XPG1_1944(xecD)
PRG: RB151_030690(budC)
PHEI: NCTC12003_01220(budC)
MMK: MU9_3089
LRI: NCTC12151_00251(fabG_2)
ADP: NCTC12871_00053(butA)
VCH: VC1591
VCS: MS6_1373
VCE: Vch1786_I1088(alsO)
VCI: O3Y_07715
VCR: VC395_1707(alsO)
VCM: VCM66_1531(alsO)
VAG: N646_4032
VAN: VAA_01317
VAU: VANGNB10_cII0928(alsO)
PAE: PA4148
PAEV: N297_4280
PAEI: N296_4280
PAEP: PA1S_04080
PAEM: U769_04105
PAEL: T223_04015
PAEG: AI22_29535
PAEC: M802_4278
PAEO: M801_4146
PCQ: PcP3B5_28830(xecD_1) PcP3B5_42460(cpnA_2) PcP3B5_43510(xecD_2)
PPX: T1E_5540 T1E_5544(budC)
PPUT: L483_14190
PPUN: PP4_27010(bdhA)
PSB: Psyr_0904
PSYR: N018_21135
PPRC: PFLCHA0_c22470(fabG7)
PPRO: PPC_2226
PFE: PSF113_2536(moaE2) PSF113_3387(ditB)
PMAN: OU5_1394
PEN: PSEEN3110
PKC: PKB_2446(moae3) PKB_4316
PSES: PSCI_3351(ucpA)
PSEC: CCOS191_2764(moaE1)
PANR: A7J50_3690
PSIL: PMA3_11780
ACX: Achr_8770
PAR: Psyc_2092
PALI: A3K91_2664
PSYA: AOT82_1902
ACB: A1S_1704
ABM: ABSDF1933(budC)
ABY: ABAYE1944(budC)
ABN: AB57_1932
ABB: ABBFA_01767(budC_1)
ABX: ABK1_2198
ABH: M3Q_2090
ABAD: ABD1_16960(budC)
ABAZ: P795_8710
ABAU: IX87_19410
ABAA: IX88_10475
ACC: BDGL_001065(budC)
ACI: ACIAD1022(budC)
ASJ: AsACE_p100042(budC)
AGU: AS4_33530(budC)
MCT: MCR_1660
MCS: DR90_298(budC)
MCAT: MC25239_01577(butA)
SFR: Sfri_0232
SSE: Ssed_2584
SPL: Spea_2131
SHL: Shal_2103
ILO: IL0792
PAT: Patl_2719
MAD: HP15_31
MBS: MRBBS_3187(fabG)
NOC: Noc_0093
NWA: Nwat_2599
HAM: HALO2446
HBE: BEI_0312(butA) BEI_2127
AMED: B224_4962
ACAV: VI35_01460
NZO: SAMEA4504057_0615(fabG_1)
ECOR: SAMEA4412678_1370(budC)
CVI: CV_2258
CNC: CNE_BB1p10260(fabG14)
BMAL: DM55_1183
BMAE: DM78_5
BMAQ: DM76_1159
BMAI: DM57_1518
BMAF: DM51_2293
BMAZ: BM44_609
BMAB: BM45_63
BPSE: BDL_1890
BPSM: BBQ_3350
BPSU: BBN_3471
BPSD: BBX_281
BPK: BBK_1378
BPSH: DR55_1018
BPSA: BBU_2049
BPSO: X996_623
BUT: X994_2613
BTE: BTH_I0053
BTQ: BTQ_72
BTJ: BTJ_2373
BTZ: BTL_315
BTV: BTHA_468
BTHE: BTN_1077
BTHM: BTRA_490
BTHA: DR62_1242
BTHL: BG87_490
BVE: AK36_2077
BCJ: BCAL1912(budC) BCAM1983
BCEW: DM40_4741
BCEO: I35_1831(budC) I35_5815
BUB: BW23_5571
BSTG: WT74_29055
BXB: DR64_7805
BPH: Bphy_6809
BFN: OI25_3848
LMIR: NCTC12852_01833(budC_2)
BPER: BN118_0922
BBR: BB2701
BHZ: ACR54_04428(cpnA_4)
TAS: TASI_0817
TAT: KUM_0033
AMIM: MIM_c38320
BPSI: IX83_03945
ODI: ODI_R3186
POL: Bpro_0097
AAV: Aave_1551
CTES: O987_07270
RTA: Rta_20970
LIH: L63ED372_02695(fabG_8)
OFO: BRW83_1378(tsaC1)
PCA: Pcar_0903(budY) Pcar_2068(budZ)
PPD: Ppro_3110
DAL: Dalk_2964
ADE: Adeh_3746
SCL: sce8810
DBR: Deba_1113
SME: SMa0329
SMER: DU99_18895
SFD: USDA257_c14870(fabG2) USDA257_c18680(budC)
ATU: Atu1905
RLE: RL2725
RLG: Rleg_2261
SHZ: shn_27055
BJA: bll5898
RPA: RPA0109
RPD: RPD_0352
RPT: Rpal_0113
XAU: Xaut_4042
AZC: AZC_1906
SNO: Snov_3725
MDI: METDI1970
MEX: Mext_1326
MCH: Mchl_1526
MPO: Mpop_0321
BID: Bind_0358
PLEO: OHA_1_01479(budC_1) OHA_1_03140(budC_2)
MMED: Mame_00462(pldh-t_2) Mame_02783(budC_2) Mame_02835(budC_3) Mame_03728(budC_4) Mame_04461(cpnA_5)
HDI: HDIA_0852(budC_1) HDIA_4498(budC_3)
RUT: FIU92_12395(fabG9)
DSH: Dshi_0620(fabG2) Dshi_4158
KVL: KVU_1138
KVU: EIO_1668
PSF: PSE_2036
PGD: Gal_04311
PHP: PhaeoP97_03857(phaB_2)
PTP: RCA23_c28470(fabG5)
MALG: MALG_04321
RHC: RGUI_2217
LABT: FIU93_26070(linC3) FIU93_27630(polS3)
RID: RIdsm_00422(cpnA_1) RIdsm_01126(tsaC1_2) RIdsm_05009(fabG_15)
ROH: FIU89_09140(fabG6)
ROT: FIV09_03625(tsaC1) FIV09_10420(budC)
HBA: Hbal_1494
ZMO: ZMO0318
ZMN: Za10_0913
ZMM: Zmob_0867
ZMB: ZZ6_0921
ZMI: ZCP4_0946
ZMC: A265_00937(cpnA)
ZMR: A254_00937(cpnA)
SGI: SGRAN_0467(budC) SGRAN_1184(yxbG)
SPHD: HY78_08930
SPHI: TS85_23075
SSAN: NX02_p1300
SECH: B18_03855
SINB: SIDU_16850
SFLA: SPHFLASMR4Y_02140(benD)
ELI: ELI_09045
ANH: A6F65_00034(linC)
GOX: GOX2036
GDI: GDI2623(budA)
GDJ: Gdia_0838
GXL: H845_1119
ASZ: ASN_1243
RRF: F11_06535
ALI: AZOLI_0733(budC)
ABS: AZOBR_50037(budC)
TMO: TMO_c0854(budC)
TXI: TH3_05530
BSR: I33_4155
BSL: A7A1_1958
BSUS: Q433_21990
BSX: C663_3904(yxbG)
BLI: BL01177
BLD: BLi02066(budC)
BMQ: BMQ_2363
BMEG: BG04_4736
BAG: Bcoa_1663
BJS: MY9_4119
BACY: QF06_18385
BEO: BEH_22585
BCL: ABC0681
BKW: BkAM31D_10020(budC)
OIH: OB3310
GKA: GK1684
GTN: GTNG_1528
VPN: A21D_01009(budC)
PASA: BAOM_0540
SAU: SA0122(butA)
SAV: SAV0126(butA) SAV2472
SAW: SAHV_0125(butA) SAHV_2456
SAM: MW0100(butA) MW2396
SAC: SACOL0111
SAE: NWMN_0071
SUK: SAA6008_00105(butA)
SUZ: MS7_0118(budC)
SUG: SAPIG0140
SAUS: SA40_0094
SAUU: SA957_0109
SAUG: SA268_0106
SAUF: X998_0106
SAUB: C248_0116
SAUM: BN843_1290
SAUC: CA347_139
SAUR: SABB_01714(butA)
SAUI: AZ30_00660
SAMS: NI36_00560
SSP: SSP0436
SCA: SCA_0065
SCAP: AYP1020_1850(butA)
MCAK: MCCS_05200(budC)
ESI: Exig_2983
EAN: Eab7_2794
PSAB: PSAB_18340
PRI: PRIO_1501
SIV: SSIL_3474
LLA: L118271(butA)
LLK: LLKF_0925(butA)
LLT: CVCAS_0859(butA)
LLS: lilo_0848(butA)
LLX: NCDO2118_0917(butA)
LLM: llmg_0339(dar) llmg_1641(butA)
LLI: uc509_0335(dar) uc509_0937(butA)
LLJ: LG36_1428(butA)
LPK: LACPI_0294(budC) LACPI_2399(butA)
SPYA: A20_0576(budC)
SPS: SPs1397
SPF: SpyM51329
STZ: SPYALAB49_000566(budC)
SPYH: L897_02865
SAG: SAG0523
SAN: gbs0569
SAK: SAK_0674
SGC: A964_0556(butA)
SAGM: BSA_6110
SAGI: MSA_6260
SAGR: SAIL_6420
SAGP: V193_02905
SAGC: DN94_02905
SAGE: EN72_03000
SAGG: EN73_03075
SAGN: W903_0700
SMU: SMU_1322(budC)
SMC: SmuNN2025_0740(budC)
SMJ: SMULJ23_0734(budC)
SMUA: SMUFR_1156
STC: str0909
STL: stu0909
STN: STND_0880
STW: Y1U_C0967
SGO: SGO_1096(butA)
SGG: SGGBAA2069_c04210(butA)
SGT: SGGB_0459(butA)
SMB: smi_0567
STK: STP_0088
STB: SGPB_0386(butA)
SCF: Spaf_0915
STJ: SALIVA_1174(butA)
SIF: Sinf_0395
SLU: KE3_0471
LSA: LCA_0292(budC)
LBN: LBUCD034_0468(butA) LBUCD034_2461(dar)
LSN: LSA_01410(budC)
LOL: LACOL_0050(butA3) LACOL_0142(butA1) LACOL_1011(butA2)
LFR: LC40_0950
LFF: LBFF_1625
PPE: PEPE_0286
LGS: LEGAS_0209(butA1) LEGAS_1299(butA2)
WKO: WKK_02035
CPAT: CLPA_c06790(butA) CLPA_c30100(bacC)
CPAE: CPAST_c06790(butA) CPAST_c30100(bacC)
ROB: CK5_32900
CSO: CLS_18240
ELM: ELI_0324
OVA: OBV_04770
MANA: MAMMFC1_03182(fabG_3)
STED: SPTER_02840(fabG_1)
APAL: BN85406040(budC)
MPA: MAP_0079
MAO: MAP4_3796
MAVI: RC58_18840
MAVU: RE97_18885
MAV: MAV_0087
MIT: OCO_00930
MIA: OCU_00960
MID: MIP_00343
MYO: OEM_01010
MIR: OCQ_00910
MUL: MUL_3168
MSHG: MSG_02417 MSG_02480(fabG_2)
MPHL: MPHLCCUG_01422(fabG_2) MPHLCCUG_03418(fabG_12)
MHAS: MHAS_03030(fabG_7)
MABB: MASS_0662
MSAL: DSM43276_00606(budC_2)
MJD: JDM601_0871(fabG3)
MTER: 4434518_00828(fabG3_3)
MMIN: MMIN_24970
ASD: AS9A_4226
CGL: NCgl2582(Cgl2674)
CGB: cg2958(butA)
CGU: WA5_2582(ButA)
CGT: cgR_2577
CGM: cgp_2958(butA)
CGJ: AR0_12965
CDX: CDES_12115(budC)
CGV: CGLAU_02765(budC)
NFA: NFA_30760
NFR: ERS450000_00989(budC_3)
RHB: NY08_4367
RFA: A3L23_00999(xecD) A3L23_02474(budC_1)
RHS: A3Q41_00892(budC_2)
RHU: A3Q40_01822(budC_1) A3Q40_02924(fabG_9)
RRT: 4535765_03839(budC_4)
GOM: D7316_03969(budC) D7316_04152(gno_2)
SCO: SCO0256(SCF20.02)
SCT: SCAT_p0291(xecE)
SBH: SBI_04380
SHY: SHJG_6880
SALS: SLNWT_6968
SLAU: SLA_0424
SLX: SLAV_34795(fabG19)
CMC: CMN_00047
MTS: MTES_0661
MOY: CVS54_01162(bacC_2) CVS54_01201(bacC_3)
ARR: ARUE_c08290(xecD)
ARX: ARZXY2_156(butA)
AAU: AAur_0876
PAUS: NCTC13651_00627(pldh-t)
MPH: MLP_06710
NDK: I601_1869(xecD_3)
KFL: Kfla_1577
MMAR: MODMU_1341
AOI: AORI_3586
AMYY: YIM_25290(budC2) YIM_25895(butA) YIM_30660(gdhI1)
AORI: SD37_18415
PSEH: XF36_14320
PAUT: Pdca_34070
ALO: CRK57834
ACTN: L083_6240
AFS: AFR_03185
BLA: BLA_1253(butA)
BBB: BIF_02081
BBC: BLC1_0697
BLV: BalV_0704
BLW: W7Y_0730
BLS: W91_0752
BANI: Bl12_0681
BANL: BLAC_03715
BANM: EN10_03690
BCOR: BCOR_1070
BSCA: BBSC_0348
PDO: PSDT_1307
ATM: ANT_25830
DGE: Dgeo_0690
TTR: Tter_1447
WCH: wcw_0542
BVO: Pan97_45980(fabG_7)
LIS: LIL_12284
LWD: JCM16777_1290(fabG_2)
SBR: SY1_02260
FSC: FSU_1055
GBA: J421_2051
SMIZ: 4412673_04020(fabG_4)
DFE: Dfer_1861
FLM: MY04_1475
MARM: YQ22_05140
CBAL: M667_17420
CBAT: M666_17420
NDO: DDD_0328
KOS: KORDIASMS9_00364(butA)
KAN: IMCC3317_10030(butA_1) IMCC3317_38190(butA_2)
KOL: Kole_0163
ASAC: ATHSA_0401
CEX: CSE_13720
NIO: NITINOP_1309(xecD)
MBAK: MSBR3_0298
SALI: L593_06440
LOKI: Lokiarch_40950(fabG_9)
 » show all
Reference
1  [PMID:8879540]
  Authors
Giovannini PP, Medici A, Bergamini CM, Rippa M
  Title
Properties of diacetyl (acetoin) reductase from Bacillus stearothermophilus.
  Journal
Bioorg Med Chem 4:1197-201 (1996)
DOI:10.1016/0968-0896(96)00086-7
Reference
2  [PMID:2040298]
  Authors
Carballo J, Martin R, Bernardo A, Gonzalez J
  Title
Purification, characterization and some properties of diacetyl(acetoin) reductase from Enterobacter aerogenes.
  Journal
Eur J Biochem 198:327-32 (1991)
DOI:10.1111/j.1432-1033.1991.tb16019.x
Reference
3
  Authors
Ui, S., Okajima, Y., Mimura, A., Kanai, H., Kobayashi, T., Kudo, T.
  Title
Sequence analysis of the gene for and characterization of D-acetoin forming meso-2,3-butanediol dehydrogenase of Klebsiella pneumoniae expressed in Escherichia coli.
  Journal
J Ferment Bioeng 83:32-37 (1997)
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.304
IUBMB Enzyme Nomenclature: 1.1.1.304
ExPASy - ENZYME nomenclature database: 1.1.1.304
BRENDA, the Enzyme Database: 1.1.1.304
LinkDB

DBGET integrated database retrieval system