KEGG   ENZYME: 1.1.1.421
Entry
EC 1.1.1.421                Enzyme                                 

Name
D-apionate oxidoisomerase;
apnO (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
D-apionate:NAD+ oxidoreductase (isomerizing)
Reaction(IUBMB)
D-apionate + NAD+ = 3-oxoisoapionate + NADH + H+ [RN:R12503]
Reaction(KEGG)
R12503
Substrate
D-apionate [CPD:C22219];
NAD+ [CPD:C00003]
Product
3-oxoisoapionate [CPD:C22220];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
The enzyme, characterized from several bacterial species, participates in the degradation of D-apionate. The reaction involves migration of a hydroxymethyl group from position 3 to position 2 and oxidation of the 3-hydroxyl group. Stereospecificity of the product, 3-oxoisoapionate, has not been determined.
History
EC 1.1.1.421 created 2019
Orthology
K23245  D-apionate oxidoisomerase
Genes
EFE: EFER_0433
AHN: NCTC12129_01617
RBAD: H2866_02220
ECA: ECA0869
PATR: EV46_03995
PATO: GZ59_08050
PCC: PCC21_007740
PCV: BCS7_03830
IZH: FEM41_02315
EBI: EbC_33080
ODI: ODI_R0403
POL: Bpro_0087
MLO: mll7016
MHUA: MCHK_0239
SME: SM_b20348
SMER: DU99_31555
SMD: Smed_3771
ARA: Arad_9232
RLE: pRL120394
RLG: Rleg_4760
SHZ: shn_27355
OAN: Oant_3065
OAH: DR92_3438
XAU: Xaut_2925
SNO: Snov_3663
MNO: Mnod_2421
MMED: Mame_04744
JAN: Jann_3066
MALU: KU6B_32130
ACR: Acry_1069
PSWU: SY83_22155
SCO: SCO0576(SC5G5.08c)
SFA: Sfla_3625
SBH: SBI_04326
STRP: F750_3092
STRD: NI25_01435
SMAL: SMALA_4776
MTS: MTES_2012
AMI: Amir_2651
ACTI: UA75_20490
ACAD: UA74_19990
TRO: trd_0130
ABAS: ACPOL_4618
 » show all
Reference
1  [PMID:29867142]
  Authors
Carter MS, Zhang X, Huang H, Bouvier JT, Francisco BS, Vetting MW, Al-Obaidi N, Bonanno JB, Ghosh A, Zallot RG, Andersen HM, Almo SC, Gerlt JA
  Title
Functional assignment of multiple catabolic pathways for D-apiose.
  Journal
Nat Chem Biol 14:696-705 (2018)
DOI:10.1038/s41589-018-0067-7
  Sequence
[ara:Arad_9232]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.421
IUBMB Enzyme Nomenclature: 1.1.1.421
ExPASy - ENZYME nomenclature database: 1.1.1.421
BRENDA, the Enzyme Database: 1.1.1.421
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