EC 18.104.22.1682 Enzyme
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
(+)-(1S,2S)-pseudoephedrine + NAD+ = (S)-2-(methylamino)-1-phenylpropan-1-one + NADH + H+ [RN:
The enzyme, characterized from the bacterium Arthrobacter sp. TS-15, acts on a broad range of different aryl-alkyl ketones, such as haloketones, ketoamines, diketones, and ketoesters. It accepts various types of aryl groups including phenyl-, pyridyl-, thienyl-, and furyl-rings, but the presence of an aromatic ring is essential for the activity. In addition, the presence of a functional group on the alkyl chain, such as an amine, a halogen, or a ketone, is also crucial. The enzyme exhibits a strict anti-Prelog enantioselectivity. When acting on diketones, it catalyses the reduction of only the keto group closest to the ring, with no further reduction to the diol. cf. EC
, ephedrine dehydrogenase.
EC 22.214.171.1242 created 2020
Shanati, T., Lockie, C., Beloti, L., Grogan, G. and Ansorge-Schumacher, M.B.
Two enantiocomplementary ephedrine dehydrogenases from Arthrobacter sp. TS-15 with broad substrate specificity.
ACS Catal 9:6202-6211 (2019)
Shanati, T., Ansorge-Schumacher, M.
Enzymes and methods for the stereoselective reduction of carbonyl compounds, oxidation and stereoselective reductive amination - for the enantioselective preparation of alcohol amine compounds.
Shanati T, Ansorge-Schumacher MB.
Biodegradation of Ephedrine Isomers by Arthrobacter sp. Strain TS-15: Discovery of Novel Ephedrine and Pseudoephedrine Dehydrogenases.
Appl Environ Microbiol 86:e02487-19 (2020)
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