EC 126.96.36.1993 Enzyme
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
(-)-(1R,2S)-ephedrine + NAD+ = (S)-2-(methylamino)-1-phenylpropan-1-one + NADH + H+ [RN:
The enzyme, characterized from the bacterium Arthrobacter sp. TS-15, acts on a broad range of different aryl-alkyl ketones, such as haloketones, ketoamines, diketones, and ketoesters. It exhibits a strict enantioselectivity and accepts various types of aryl groups including phenyl-, pyridyl-, thienyl-, and furyl-rings, but the presence of an aromatic ring is essential for the activity. In addition, the presence of a functional group on the alkyl chain, such as an amine, a halogen, or a ketone, is also crucial. When acting on diketones, it catalyses the reduction of only the keto group closest to the ring, with no further reduction to the diol. cf. EC
, pseudoephedrine dehydrogenase and EC
, ephedrine dehydrogenase.
EC 188.8.131.523 created 2020, modified 2020
Shanati, T., Lockie, C., Beloti, L., Grogan, G. and Ansorge-Schumacher, M.B.
Two enantiocomplementary ephedrine dehydrogenases from Arthrobacter sp. TS-15 with broad substrate specificity.
ACS Catal 9:6202-6211 (2019)
Shanati, T., Ansorge-Schumacher, M.
Enzymes and methods for the stereoselective reduction of carbonyl compounds, oxidation and stereoselective reductive amination - for the enantioselective preparation of alcohol amine compounds.
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