KEGG   ENZYME: 1.13.11.74
Entry
EC 1.13.11.74               Enzyme                                 

Name
2-aminophenol 1,6-dioxygenase;
amnA (gene name);
amnB (gene name);
2-aminophenol:oxygen 1,6-oxidoreductase (decyclizing)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
Sysname
2-aminophenol:oxygen 1,6-oxidoreductase (ring-opening)
Reaction(IUBMB)
2-aminophenol + O2 = 2-aminomuconate 6-semialdehyde [RN:R05405]
Reaction(KEGG)
R05405
Substrate
2-aminophenol [CPD:C01987];
O2 [CPD:C00007]
Product
2-aminomuconate 6-semialdehyde [CPD:C03824]
Comment
The enzyme, a member of the nonheme-iron(II)-dependent dioxygenase family, is an extradiol-type dioxygenase that utilizes a non-heme ferrous iron to cleave the aromatic ring at the meta position (relative to the hydroxyl substituent). The enzyme also has some activity with 2-amino-5-methylphenol and 2-amino-4-methylphenol [1]. The enzyme from the bacterium Comamonas testosteroni CNB-1 also has the activity of EC 1.13.11.76, 2-amino-5-chlorophenol 1,6-dioxygenase [2].
History
EC 1.13.11.74 created 2013
Pathway
ec00627  Aminobenzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K15059  2-aminophenol/2-amino-5-chlorophenol 1,6-dioxygenase subunit beta
Genes
PPW: PputW619_2014
PKC: PKB_3263(amnb1) PKB_3611(amnb3)
BXE: Bxe_A1145(amnB)
BXB: DR64_3304(amnB)
BAV: BAV0550(amnB)
BHZ: ACR54_00822(cnbCb)
AXY: AXYL_02641
AIS: BUW96_03280
ASW: CVS48_11345
ACHB: DVB37_23465
 » show all
Reference
1  [PMID:9169437]
  Authors
Takenaka S, Murakami S, Shinke R, Hatakeyama K, Yukawa H, Aoki K
  Title
Novel genes encoding 2-aminophenol 1,6-dioxygenase from Pseudomonas species AP-3  growing on 2-aminophenol and catalytic properties of the purified enzyme.
  Journal
J Biol Chem 272:14727-32 (1997)
DOI:10.1074/jbc.272.23.14727
  Sequence
Reference
2  [PMID:15580337]
  Authors
Wu JF, Sun CW, Jiang CY, Liu ZP, Liu SJ
  Title
A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli.
  Journal
Arch Microbiol 183:1-8 (2005)
DOI:10.1007/s00203-004-0738-5
  Sequence
Reference
3  [PMID:23275161]
  Authors
Li de F, Zhang JY, Hou YJ, Liu L, Hu Y, Liu SJ, Wang da C, Liu W
  Title
Structures of aminophenol dioxygenase in complex with intermediate, product and inhibitor.
  Journal
Acta Crystallogr D Biol Crystallogr 69:32-43 (2013)
DOI:10.1107/S0907444912042072
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.74
IUBMB Enzyme Nomenclature: 1.13.11.74
ExPASy - ENZYME nomenclature database: 1.13.11.74
BRENDA, the Enzyme Database: 1.13.11.74
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