Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

9H-carbazole,NAD(P)H:oxygen oxidoreductase (2,3-hydroxylating)

9H-carbazole + NAD(P)H + H+ + O2 = 2'-aminobiphenyl-2,3-diol + NAD(P)+ [RN:R05414 R09512]

R05414 R09512

9H-carbazole [CPD:C08060];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

This enzyme catalyses the first reaction in the pathway of carbazole degradation. The enzyme attacks at the 1 and 9a positions of carbazole, resulting in the formation of a highly unstable hemiaminal intermediate that undergoes a spontaneous cleavage and rearomatization, resulting in 2'-aminobiphenyl-2,3-diol. In most bacteria the enzyme is a complex composed of a terminal oxygenase, a ferredoxin, and a ferredoxin reductase. The terminal oxygenase component contains a nonheme iron centre and a Rieske [2Fe-2S] iron-sulfur cluster.

EC 1.14.12.22 created 2010

1  [PMID:12450807]

Nam JW, Nojiri H, Noguchi H, Uchimura H, Yoshida T, Habe H, Yamane H, Omori T

Purification and characterization of carbazole 1,9a-dioxygenase, a three-component dioxygenase system of Pseudomonas resinovorans strain CA10.

Appl Environ Microbiol 68:5882-90 (2002)
DOI:10.1128/AEM.68.12.5882-5890.2002

[pre:PCA10_p0280]

2  [PMID:20368802]

Gai Z, Wang X, Liu X, Tai C, Tang H, He X, Wu G, Deng Z, Xu P

The genes coding for the conversion of carbazole to catechol are flanked by IS6100 elements in Sphingomonas sp. strain XLDN2-5.

PLoS One 5:e10018 (2010)
DOI:10.1371/journal.pone.0010018

[ag:ADC31794]