Entry |
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Name |
carbazole 1,9a-dioxygenase;
CARDO
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
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Sysname |
9H-carbazole,NAD(P)H:oxygen oxidoreductase (2,3-hydroxylating)
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Reaction(IUBMB) |
9H-carbazole + NAD(P)H + H+ + O2 = 2'-aminobiphenyl-2,3-diol + NAD(P)+ [RN: R05414 R09512]
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
This enzyme catalyses the first reaction in the pathway of carbazole degradation. The enzyme attacks at the 1 and 9a positions of carbazole, resulting in the formation of a highly unstable hemiaminal intermediate that undergoes a spontaneous cleavage and rearomatization, resulting in 2'-aminobiphenyl-2,3-diol. In most bacteria the enzyme is a complex composed of a terminal oxygenase, a ferredoxin, and a ferredoxin reductase. The terminal oxygenase component contains a nonheme iron centre and a Rieske [2Fe-2S] iron-sulfur cluster.
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History |
EC 1.14.12.22 created 2010
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Pathway |
ec01120 | Microbial metabolism in diverse environments |
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Orthology |
K15751 | carbazole 1,9a-dioxygenase |
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Genes |
» show all
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Reference |
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Authors |
Nam JW, Nojiri H, Noguchi H, Uchimura H, Yoshida T, Habe H, Yamane H, Omori T |
Title |
Purification and characterization of carbazole 1,9a-dioxygenase, a three-component dioxygenase system of Pseudomonas resinovorans strain CA10. |
Journal |
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Sequence |
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Reference |
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Authors |
Gai Z, Wang X, Liu X, Tai C, Tang H, He X, Wu G, Deng Z, Xu P |
Title |
The genes coding for the conversion of carbazole to catechol are flanked by IS6100 elements in Sphingomonas sp. strain XLDN2-5. |
Journal |
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Sequence |
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Other DBs |
UM-BBD (Biocatalysis/Biodegradation Database): | 1.14.12.22 |
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LinkDB |
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