Entry |
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Name |
nitroarene dioxygenase;
cnbA (gene name)
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
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Sysname |
nitrobenzene,NADH:oxygen oxidoreductase (1,2-hydroxylating, nitrite-releasing)
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Reaction(IUBMB) |
nitrobenzene + NADH + O2 = catechol + nitrite + NAD+ [RN: R07706]
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
This enzyme is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin---NAD+ reductase), and an alpha3beta3 oxygenase. The enzyme forms of a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It can typically act on many different nitroaromatic compounds, including chlorinated species. Enzymes found in different strains may have different substrate preferences. Requires Fe2+.
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History |
EC 1.14.12.23 created 2015
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Pathway |
ec01120 | Microbial metabolism in diverse environments |
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Orthology |
K14579 | naphthalene 1,2-dioxygenase subunit alpha |
K14580 | naphthalene 1,2-dioxygenase subunit beta |
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Genes |
» show all
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Reference |
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Authors |
Parales JV, Parales RE, Resnick SM, Gibson DT |
Title |
Enzyme specificity of 2-nitrotoluene 2,3-dioxygenase from Pseudomonas sp. strain JS42 is determined by the C-terminal region of the alpha subunit of the oxygenase component. |
Journal |
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Reference |
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Authors |
Lessner DJ, Johnson GR, Parales RE, Spain JC, Gibson DT |
Title |
Molecular characterization and substrate specificity of nitrobenzene dioxygenase from Comamonas sp. strain JS765. |
Journal |
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Sequence |
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Reference |
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Authors |
Liu H, Wang SJ, Zhang JJ, Dai H, Tang H, Zhou NY |
Title |
Patchwork assembly of nag-like nitroarene dioxygenase genes and the 3-chlorocatechol degradation cluster for evolution of the 2-chloronitrobenzene catabolism pathway in Pseudomonas stutzeri ZWLR2-1. |
Journal |
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Sequence |
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Reference |
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Authors |
Singh D, Kumari A, Ramaswamy S, Ramanathan G |
Title |
Expression, purification and substrate specificities of 3-nitrotoluene dioxygenase from Diaphorobacter sp. strain DS2. |
Journal |
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Sequence |
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Other DBs |
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LinkDB |
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