KEGG ENZYME: 1.14.12.23

ENZYME: 1.14.12.23

Entry

EC 1.14.12.23 Enzyme

Name

nitroarene dioxygenase;
cnbA (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor

Sysname

nitrobenzene,NADH:oxygen oxidoreductase (1,2-hydroxylating, nitrite-releasing)

Reaction(IUBMB)

nitrobenzene + NADH + O2 = catechol + nitrite + NAD+ [RN:R07706]

Reaction(KEGG)

R07706

Substrate

nitrobenzene [CPD:C06813];
NADH [CPD:C00004];
O2 [CPD:C00007]

Product

catechol [CPD:C00090];
nitrite [CPD:C00088];
NAD+ [CPD:C00003]

Comment

This enzyme is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin---NAD+ reductase), and an alpha3beta3 oxygenase. The enzyme forms of a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It can typically act on many different nitroaromatic compounds, including chlorinated species. Enzymes found in different strains may have different substrate preferences. Requires Fe2+.

History

EC 1.14.12.23 created 2015

Pathway

ec00627

Aminobenzoate degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K14579

naphthalene 1,2-dioxygenase subunit alpha

K14580

naphthalene 1,2-dioxygenase subunit beta

Genes

PPI:	ND055(nahAc) ND056(nahAd)

PSC:	A458_06720 A458_06725

PSTU:

UIB01_22625 UIB01_22630

PBM:	CL52_14610 CL52_14615

PFK:	PFAS1_28610 PFAS1_28615

ALT:	ambt_17890 ambt_17895

RMN:	TK49_07955

BMK:	DM80_1443(ndoB) DM80_1444(ndoC)

PARB:

CJU94_38655 CJU94_38660

PNA:	Pnap_2484 Pnap_2485

AJS:	Ajs_3092 Ajs_3093

» show all

Reference

1 [PMID:9495758]

Authors

Parales JV, Parales RE, Resnick SM, Gibson DT

Title

Enzyme specificity of 2-nitrotoluene 2,3-dioxygenase from Pseudomonas sp. strain JS42 is determined by the C-terminal region of the alpha subunit of the oxygenase component.

Journal

J Bacteriol 180:1194-9 (1998)

Reference

2 [PMID:11823201]

Authors

Lessner DJ, Johnson GR, Parales RE, Spain JC, Gibson DT

Title

Molecular characterization and substrate specificity of nitrobenzene dioxygenase from Comamonas sp. strain JS765.

Journal

Appl Environ Microbiol 68:634-41 (2002)
DOI:10.1128/AEM.68.2.634-641.2002

Sequence

[ag:AAL76202 AAL76203]

Reference

3 [PMID:21602392]

Authors

Liu H, Wang SJ, Zhang JJ, Dai H, Tang H, Zhou NY

Title

Patchwork assembly of nag-like nitroarene dioxygenase genes and the 3-chlorocatechol degradation cluster for evolution of the 2-chloronitrobenzene catabolism pathway in Pseudomonas stutzeri ZWLR2-1.

Journal

Appl Environ Microbiol 77:4547-52 (2011)
DOI:10.1128/AEM.02543-10

Sequence

[ag:ADQ90222 ADQ90223]

Reference

4 [PMID:24491551]

Authors

Singh D, Kumari A, Ramaswamy S, Ramanathan G

Title

Expression, purification and substrate specificities of 3-nitrotoluene dioxygenase from Diaphorobacter sp. strain DS2.

Journal

Biochem Biophys Res Commun 445:36-42 (2014)
DOI:10.1016/j.bbrc.2014.01.113