Entry |
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Name |
2,4-dinitrotoluene dioxygenase;
dntA (gene name)
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
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Sysname |
2,4-dinitrotoluene,NADH:oxygen oxidoreductase (4,5-hydroxylating, nitrite-releasing)
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Reaction(IUBMB) |
2,4-dinitrotoluene + NADH + O2 = 4-methyl-5-nitrocatechol + nitrite + NAD+ [RN: R09233]
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
This enzyme, characterized from the bacterium Burkholderia sp. strain DNT, is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin---NAD+ reductase), and an alpha3beta3 oxygenase. The enzyme forms a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It does not act on nitrobenzene. cf. EC 1.14.12.23, nitroarene dioxygenase.
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History |
EC 1.14.12.24 created 2015
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Pathway |
ec01120 | Microbial metabolism in diverse environments |
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Orthology |
K14579 | naphthalene 1,2-dioxygenase subunit alpha |
K14580 | naphthalene 1,2-dioxygenase subunit beta |
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Genes |
» show all
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Reference |
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Authors |
Suen WC, Haigler BE, Spain JC |
Title |
2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase. |
Journal |
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Sequence |
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Other DBs |
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LinkDB |
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