Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy

chlorobenzene,NADH:oxygen oxidoreductase (1,2-hydroxylating)

chlorobenzene + NADH + H+ + O2 = (1R,2R)-3-chlorocyclohexa-3,5-diene-1,2-diol + NAD+ [RN:R06856]

R06856;
(other) R05429 R06831
Reaction

chlorobenzene [CPD:C06990];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]

This bacterial enzyme is a class IIB dioxygenase, comprising three components - a heterodimeric terminal dioxygenase, a ferredoxin protein, and a ferredoxin reductase. The enzyme acts on a range of aromatic compounds, including mono-, di-, tri-, and tetra-chlorinated benzenes and toluenes.

EC 1.14.12.26 created 2018

1  [PMID:8526500]

Spiess E, Sommer C, Gorisch H

Degradation of 1,4-dichlorobenzene by Xanthobacter flavus 14p1.

Appl Environ Microbiol 61:3884-8 (1995)
DOI:10.1128/AEM.61.11.3884-3888.1995

2  [PMID:9148781]

Sommer C, Gorisch H.

Enzymology of the degradation of (di)chlorobenzenes by Xanthobacter flavus 14p1.

Arch Microbiol 167:384-91 (1997)
DOI:10.1007/s002030050459

3  [PMID:9249026]

Beil S, Happe B, Timmis KN, Pieper DH

Genetic and biochemical characterization of the broad spectrum chlorobenzene dioxygenase from Burkholderia sp. strain PS12--dechlorination of 1,2,4,5-tetrachlorobenzene.

Eur J Biochem 247:190-9 (1997)
DOI:10.1111/j.1432-1033.1997.00190.x

4  [PMID:9791099]

Beil S, Mason JR, Timmis KN, Pieper DH

Identification of chlorobenzene dioxygenase sequence elements involved in dechlorination of 1,2,4,5-tetrachlorobenzene.

J Bacteriol 180:5520-8 (1998)
DOI:10.1128/JB.180.21.5520-5528.1998