| Entry |
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| Name |
(2,2,3-trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA 1,5-monooxygenase;
2-oxo-Delta3-4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase;
2-oxo-Delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase;
OTEMO
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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| Sysname |
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA,NADPH:oxygen oxidoreductase (1,5-lactonizing)
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| Reaction(IUBMB) |
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA + O2 + NADPH + H+ = [(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA + NADP+ + H2O [RN: R10044]
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| Reaction(KEGG) |
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| Substrate |
[(1R)-2,2,3-trimethyl-5-oxocyclopent-3-enyl]acetyl-CoA [CPD: C07160];
O2 [CPD: C00007];
NADPH [CPD: C00005];
H+ [CPD: C00080]
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| Product |
[(2R)-3,3,4-trimethyl-6-oxo-3,6-dihydro-1H-pyran-2-yl]acetyl-CoA [CPD: C20321];
NADP+ [CPD: C00006];
H2O [CPD: C00001]
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| Comment |
A FAD dependent enzyme isolated from Pseudomonas putida. Forms part of the catabolism pathway of camphor. It acts on the CoA ester in preference to the free acid.
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| History |
EC 1.14.13.160 created 2012
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| Orthology |
| K21730 | (2,2,3-trimethyl-5-oxocyclopent-3-enyl)acetyl-CoA 1,5-monooxygenase |
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| Genes |
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| Reference |
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| Authors |
Ougham HJ, Taylor DG, Trudgill PW |
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| Title |
Camphor revisited: involvement of a unique monooxygenase in metabolism of 2-oxo-delta 3-4,5,5-trimethylcyclopentenylacetic acid by Pseudomonas putida. |
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| Journal |
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| Reference |
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| Authors |
Leisch H, Shi R, Grosse S, Morley K, Bergeron H, Cygler M, Iwaki H, Hasegawa Y, Lau PC |
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| Title |
Cloning, Baeyer-Villiger biooxidations, and structures of the camphor pathway 2-oxo-Delta(3)-4,5,5-trimethylcyclopentenylacetyl-coenzyme A monooxygenase of Pseudomonas putida ATCC 17453. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Kadow M, Loschinski K, Sass S, Schmidt M, Bornscheuer UT |
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| Title |
Completing the series of BVMOs involved in camphor metabolism of Pseudomonas putida NCIMB 10007 by identification of the two missing genes, their functional expression in E. coli, and biochemical characterization. |
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| Journal |
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| Sequence |
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| Other DBs |
| UM-BBD (Biocatalysis/Biodegradation Database): | 1.14.13.160 |
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| LinkDB |
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