Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

4-nitrophenol,NAD(P)H:oxygen 4-oxidoreductase (4-hydroxylating, nitrite-forming)

4-nitrophenol + NADPH + H+ + O2 = 1,4-benzoquinone + nitrite + NADP+ + H2O [RN:R05258]

R05258
Reaction

4-nitrophenol [CPD:C00870];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Contains FAD. The enzyme catalyses the first step in a degradation pathway for 4-nitrophenol, the oxidation of 4-nitrophenol at position 4 with the concomitant removal of the nitro group as nitrite. The enzyme from the bacterium Pseudomonas sp. strain WBC-3 also catalyses EC 1.14.13.166, 4-nitrocatechol 4-monooxygenase.

EC 1.14.13.167 created 2012

1  [PMID:19218392]

Zhang JJ, Liu H, Xiao Y, Zhang XE, Zhou NY

Identification and characterization of catabolic para-nitrophenol 4-monooxygenase and para-benzoquinone reductase from Pseudomonas sp. strain WBC-3.

J Bacteriol 191:2703-10 (2009)
DOI:10.1128/JB.01566-08

[ag:ABU50908]