KEGG   ENZYME: 1.14.13.172Help
Entry
EC 1.14.13.172              Enzyme                                 

Name
salicylate 5-hydroxylase;
nagG (gene name);
nagH (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
salicylate,NADH:oxygen oxidoreductase (5-hydroxylating)
Reaction(IUBMB)
salicylate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O [RN:R07709]
Reaction(KEGG)
R07709;
(other) R07710
Show
Substrate
salicylate [CPD:C00805];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxybenzoate [CPD:C00628];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
This enzyme, which was characterized from the bacterium Ralstonia sp. U2, comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.7, ferredoxin---NAD(P)+ reductase), an iron-sulfur oxygenase, and ferredoxin.
History
EC 1.14.13.172 created 2013
Pathway
ec00626  Naphthalene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K18242  salicylate 5-hydroxylase large subunit
K18243  salicylate 5-hydroxylase small subunit
Genes
EBT: EBL_c34270 EBL_c34280
PLU: plu1000 plu1001
PLUM: A4R40_04995 A4R40_05000
PTT: VY86_00150 VY86_00155
ALT: ambt_18005 ambt_18010
TTU: TERTU_1474 TERTU_1475
MMW: Mmwyl1_2669 Mmwyl1_2670
MME: Marme_2404 Marme_2405
MPC: Mar181_2357 Mar181_2358
GBI: PG2T_03615
RSO: RSc1089(nagH) RSc1090(nagG)
RSL: RPSI07_2301 RPSI07_2302
RSN: RSPO_c02274 RSPO_c02275(nagH)
RSM: CMR15_20208 CMR15_20209
RSE: F504_1060(ohbA) F504_1061(ohbB)
RSY: RSUY_15110 RSUY_15120
RPJ: N234_28705 N234_28710
RMN: TK49_07930 TK49_07935
RPU: CDC45_05590 CDC45_05595
REU: Reut_B4719 Reut_B4720
REH: H16_A1632(nagH) H16_A1633(nagG)
CNC: CNE_1c16400(nagH) CNE_1c16410(nagG)
CUH: BJN34_29220 BJN34_29225
RME: Rmet_3712(nagH)
CBW: RR42_s2751 RR42_s2752
CCUP: BKK81_31195 BKK81_31200
CUP: BKK80_25150 BKK80_25155
CUU: BKK79_30860 BKK79_30865
COX: E0W60_10140 E0W60_10145
BMK: DM80_1440 DM80_1441
BCEP: APZ15_32865 APZ15_32870
BPYR: ABD05_24780 ABD05_24785
BUB: BW23_5815 BW23_5816
BGL: bglu_2g20110 bglu_2g20120
BGU: KS03_3913 KS03_3914
BUG: BC1001_0576 BC1001_0577
BUQ: AC233_10900 AC233_10905
BUZ: AYM40_33040 AYM40_33045 AYM40_33150 AYM40_33155
BPX: BUPH_04466 BUPH_04467
BFN: OI25_3046 OI25_3047
PSPW: BJG93_04755
PARB: CJU94_38670 CJU94_38675
PTER: C2L65_39070 C2L65_39075
PPK: U875_04295 U875_04300
PPNO: DA70_21370 DA70_21375
PPNM: LV28_08945 LV28_08950
PRB: X636_05145 X636_05150
PPUL: RO07_03175 RO07_03180
PSPU: NA29_24085 NA29_24090
PAPI: SG18_03365 SG18_03370
PVE: UC34_19330 UC34_19335
POX: MB84_24085 MB84_24090
PFG: AB870_06325 AB870_06330
PNR: AT302_02760 AT302_02765
PAND: DRB87_17855 DRB87_17860
BPT: Bpet2804 Bpet2805
AXY: AXYL_00938 AXYL_00939 AXYL_06581(bphA)
AXO: NH44784_011911 NH44784_011921
AXN: AX27061_0965 AX27061_0966
AXX: ERS451415_00956(andAd_1) ERS451415_00957(andAc_1)
ADT: APT56_05010 APT56_05015
AIS: BUW96_21015 BUW96_21020
ASW: CVS48_01680 CVS48_01685
ACHR: C2U31_09370
ACHB: DVB37_04180 DVB37_04185
PIG: EGT29_23975 EGT29_23980
POL: Bpro_0983 Bpro_0984
PNA: Pnap_2487 Pnap_2488
POS: DT070_14060 DT070_14065
AAV: Aave_0165 Aave_0166
AJS: Ajs_3090
AAA: Acav_0196 Acav_0197
ACK: C380_09995 C380_10000
VAP: Vapar_5348 Vapar_5349
VPE: Varpa_2933 Varpa_2934
VPD: VAPA_1c07030 VAPA_1c07040 VAPA_1c20400 VAPA_1c20410
VBO: CKY39_17840 CKY39_17845
VAM: C4F17_05690 C4F17_05695
RTA: Rta_25980 Rta_25990
LIM: L103DPR2_01957(nagH) L103DPR2_01958(nagG_2)
LIH: L63ED372_02714(nagH) L63ED372_02715(nagG_2)
HYR: BSY239_1027 BSY239_1029
HYB: Q5W_19150 Q5W_19155
HYL: LPB072_20440 LPB072_20445
HPSE: HPF_02890(nagG) HPF_02895(nagH)
JAJ: EKL02_16425 EKL02_16430
MASW: AM586_27630 AM586_27635 AM586_27695 AM586_27700 AM586_27790 AM586_27795
LCH: Lcho_0796 Lcho_0797
AZO: azo2515(nagH) azo2516(nagG)
AOA: dqs_2672 dqs_2673
AZR: CJ010_15880 CJ010_15885
CFH: C1707_18700 C1707_18705
SWI: Swit_3056 Swit_3057
SPHD: HY78_10290 HY78_10295
BLAS: BSY18_3955 BSY18_3956
AZL: AZL_a09200 AZL_a09210
ALI: AZOLI_p20648 AZOLI_p20649
TMO: TMO_a0553 TMO_a0554(bphA)
FER: FNB15_08990 FNB15_08995
ACTI: UA75_12800
ACAD: UA74_12720
AHG: AHOG_11995(nagG)
 » show all
Taxonomy
Reference
1  [PMID:9573207]
  Authors
Fuenmayor SL, Wild M, Boyes AL, Williams PA
  Title
A gene cluster encoding steps in conversion of naphthalene to gentisate in Pseudomonas sp. strain U2.
  Journal
J Bacteriol 180:2522-30 (1998)
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.172
IUBMB Enzyme Nomenclature: 1.14.13.172
ExPASy - ENZYME nomenclature database: 1.14.13.172
BRENDA, the Enzyme Database: 1.14.13.172
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