| Entry |
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| Name |
4-hydroxybenzoate 3-monooxygenase;
p-hydroxybenzoate hydrolyase;
p-hydroxybenzoate hydroxylase;
4-hydroxybenzoate 3-hydroxylase;
4-hydroxybenzoate monooxygenase;
4-hydroxybenzoic hydroxylase;
p-hydroxybenzoate-3-hydroxylase;
p-hydroxybenzoic acid hydrolase;
p-hydroxybenzoic acid hydroxylase;
p-hydroxybenzoic hydroxylase
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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| Sysname |
4-hydroxybenzoate,NADPH:oxygen oxidoreductase (3-hydroxylating)
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| Reaction(IUBMB) |
4-hydroxybenzoate + NADPH + H+ + O2 = 3,4-dihydroxybenzoate + NADP+ + H2O [RN: R01298]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
A flavoprotein (FAD). Most enzymes from Pseudomonas are highly specific for NADPH (cf. EC 1.14.13.33 4-hydroxybenzoate 3-monooxygenase [NAD(P)H]).
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| History |
EC 1.14.13.2 created 1972, modified 1999
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| Pathway |
| ec01120 | Microbial metabolism in diverse environments |
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| Orthology |
| K00481 | p-hydroxybenzoate 3-monooxygenase |
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| Genes |
» show all
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| Reference |
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| Authors |
Hosokawa K, Stanier RY. |
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| Title |
Crystallization and properties of p-hydroxybenzoate hydroxylase from Pseudomonas putida. |
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| Journal |
J Biol Chem 241:2453-60 (1966) |
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| Reference |
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| Authors |
Howell LG, Spector T, Massey V. |
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| Title |
Purification and properties of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. |
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| Journal |
J Biol Chem 247:4340-50 (1972) |
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| Reference |
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| Authors |
Spector T, Massey V. |
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| Title |
Studies on the effector specificity of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. |
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| Journal |
J Biol Chem 247:4679-87 (1972) |
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| Reference |
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| Authors |
Spector T, Massey V. |
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| Title |
p-Hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Evidence for an oxygenated flavin intermediate. |
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| Journal |
J Biol Chem 247:5632-6 (1972) |
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| Reference |
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| Authors |
Spector T, Massey V. |
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| Title |
p-Hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Reactivity with oxygen. |
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| Journal |
J Biol Chem 247:7123-7 (1972) |
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| Reference |
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| Authors |
Seibold B, Matthes M, Eppink MH, Lingens F, Van Berkel WJ, Muller R |
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| Title |
4-Hydroxybenzoate hydroxylase from Pseudomonas sp. CBS3. Purification, characterization, gene cloning, sequence analysis and assignment of structural features determining the coenzyme specificity. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 1.14.13.2 |
| UM-BBD (Biocatalysis/Biodegradation Database): | 1.14.13.2 |
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| LinkDB |
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