Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

asperlicin C,NAD(P)H:oxygen oxidoreductase

asperlicin C + NAD(P)H + H+ + O2 = asperlicin E + NAD(P)+ + H2O [RN:R11156 R11157]

R11156 R11157
Reaction

asperlicin C [CPD:C21147];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

The enzyme, characterized from the fungus Aspergillus alliaceus, contains an FAD cofactor. The enzyme inserts a hydroxyl group, leading to formation of a N-C bond that creates an additional cycle between the bicyclic indole and the tetracyclic core moieties, resulting in the heptacyclic asperlicin E.

EC 1.14.13.216 created 2016

1  [PMID:23030663]

Haynes SW, Gao X, Tang Y, Walsh CT

Assembly of asperlicin peptidyl alkaloids from anthranilate and tryptophan: a two-enzyme pathway generates heptacyclic scaffold complexity in asperlicin E.

J Am Chem Soc 134:17444-7 (2012)
DOI:10.1021/ja308371z