Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

5-methylphenazine-1-carboxylate,NADH:oxygen oxidoreductase (1-hydroxylating, decarboxylating)

5-methylphenazine-1-carboxylate + NADH + O2 = pyocyanin + NAD+ + CO2 + H2O [RN:R11346]

R11346;
(other) R11578

5-methylphenazine-1-carboxylate [CPD:C21295];
NADH [CPD:C00004];
O2 [CPD:C00007]

The enzyme, characterized from the bacterium Pseudomonas aeruginosa, is involved in the biosynthesis of pyocyanin, a toxin produced and secreted by the organism. It can also act on phenazine-1-carboxylate, converting it into phenazin-1-ol.

EC 1.14.13.218 created 2016

1  [PMID:11591691]

Mavrodi DV, Bonsall RF, Delaney SM, Soule MJ, Phillips G, Thomashow LS

Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1.

J Bacteriol 183:6454-65 (2001)
DOI:10.1128/JB.183.21.6454-6465.2001

[pae:PA4217]

2  [PMID:17253782]

Parsons JF, Greenhagen BT, Shi K, Calabrese K, Robinson H, Ladner JE

Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa.

Biochemistry 46:1821-8 (2007)
DOI:10.1021/bi6024403

3  [PMID:18416536]

Greenhagen BT, Shi K, Robinson H, Gamage S, Bera AK, Ladner JE, Parsons JF

Crystal structure of the pyocyanin biosynthetic protein PhzS.

Biochemistry 47:5281-9 (2008)
DOI:10.1021/bi702480t

[pae:PA4217]