Entry |
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Name |
phenol 2-monooxygenase (NADH);
dmpLMNOP (gene names)
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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Sysname |
phenol,NADH:oxygen oxidoreductase (2-hydroxylating)
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Reaction(IUBMB) |
phenol + NADH + H+ + O2 = catechol + NAD+ + H2O [RN: R10043]
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Reaction(KEGG) |
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Substrate |
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Product |
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Comment |
The enzyme, characterized from the bacteria Pseudomonas sp. CF600 and Acinetobacter radioresistens, consists of a multisubunit oxygenease component that contains the active site and a dinuclear iron center, a reductase component that contains FAD and one iron-sulfur cluster, and a regulatory component. The reductase component is responsible for transferring electrons from NADH to the dinuclear iron center.
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History |
EC 1.14.13.244 created 2019
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Pathway |
ec00361 | Chlorocyclohexane and chlorobenzene degradation |
ec01120 | Microbial metabolism in diverse environments |
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Orthology |
K16242 | phenol/toluene 2-monooxygenase (NADH) P3/A3 |
K16243 | phenol/toluene 2-monooxygenase (NADH) P1/A1 |
K16244 | phenol/toluene 2-monooxygenase (NADH) P2/A2 |
K16245 | phenol/toluene 2-monooxygenase (NADH) P4/A4 |
K16246 | phenol/toluene 2-monooxygenase (NADH) P5/A5 |
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Genes |
» show all
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Reference |
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Authors |
Nordlund I, Powlowski J, Shingler V |
Title |
Complete nucleotide sequence and polypeptide analysis of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600. |
Journal |
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Reference |
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Authors |
Powlowski J, Shingler V |
Title |
In vitro analysis of polypeptide requirements of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600. |
Journal |
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Reference |
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Authors |
Powlowski J, Sealy J, Shingler V, Cadieux E |
Title |
On the role of DmpK, an auxiliary protein associated with multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600. |
Journal |
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Reference |
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Authors |
Qian H, Edlund U, Powlowski J, Shingler V, Sethson I |
Title |
Solution structure of phenol hydroxylase protein component P2 determined by NMR spectroscopy. |
Journal |
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Reference |
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Authors |
Cadieux E, Vrajmasu V, Achim C, Powlowski J, Munck E |
Title |
Biochemical, Mossbauer, and EPR studies of the diiron cluster of phenol hydroxylase from Pseudomonas sp. strain CF 600. |
Journal |
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Other DBs |
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LinkDB |
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