EC 220.127.116.11 Enzyme
4-hydroxybenzoate 3-monooxygenase [NAD(P)H];
4-hydroxybenzoate 3-monooxygenase (reduced nicotinamide adenine dinucleotide (phosphate));
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
4-hydroxybenzoate,NAD(P)H:oxygen oxidoreductase (3-hydroxylating)
4-hydroxybenzoate + NAD(P)H + H+ + O2 = 3,4-dihydroxybenzoate + NAD(P)+ + H2O [RN:
A flavoprotein (FAD). The enzyme from Corynebacterium cyclohexanicum is highly specific for 4-hydroxybenzoate, but uses NADH and NADPH at approximately equal rates (cf. EC
4-hydroxybenzoate 3-monooxygenase). It is less specific for NADPH than EC
EC 18.104.22.168 created 1989, modified 1999
Microbial metabolism in diverse environments
Fujii T, Kaneda T.
Purification and properties of NADH/NADPH-dependent p-hydroxybenzoate hydroxylase from Corynebacterium cyclohexanicum.
Eur J Biochem 147:97-104 (1985)
Seibold B, Matthes M, Eppink MH, Lingens F, Van Berkel WJ, Muller R.
4-Hydroxybenzoate hydroxylase from Pseudomonas sp. CBS3. Purification, characterization, gene cloning, sequence analysis and assignment of structural features determining the coenzyme specificity.
Eur J Biochem 239:469-78 (1996)
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