Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

anhydrotetracycline,NADPH:oxygen oxidoreductase (6-hydroxylating)

anhydrotetracycline + NADPH + H+ + O2 = 12-dehydrotetracycline + NADP+ + H2O [RN:R04060]

R04060;
(other) R09193
Reaction

anhydrotetracycline [CPD:C02811];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. It can also catalyse EC 1.14.13.234, 12-dehydrotetracycline 5-monooxygenase.

EC 1.14.13.38 created 1990, modified 2016

1

Behal, V., Hostalek, Z. and Vanek, Z.

Anhydrotetracycline oxygenase activity and biosynthesis of tetracyclines in streptomyces aureofaciens.

Biotechnol Lett 1:177-182 (1979)

2  [PMID:2914874]

Binnie C, Warren M, Butler MJ

Cloning and heterologous expression in Streptomyces lividans of Streptomyces rimosus genes involved in oxytetracycline biosynthesis.

J Bacteriol 171:887-95 (1989)
DOI:10.1128/JB.171.2.887-895.1989

3  [PMID:3138982]

Vancurova I, Volc J, Flieger M, Neuzil J, Novotna J, Vlach J, Behal V

Isolation of pure anhydrotetracycline oxygenase from Streptomyces aureofaciens.

Biochem J 253:263-7 (1988)
DOI:10.1042/bj2530263

4  [PMID:23621493]

Wang P, Bashiri G, Gao X, Sawaya MR, Tang Y

Uncovering the enzymes that catalyze the final steps in oxytetracycline biosynthesis.

J Am Chem Soc 135:7138-41 (2013)
DOI:10.1021/ja403516u