| Entry |
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| Name |
L-lysine N6-monooxygenase (NADPH);
lysine N6-hydroxylase;
L-lysine 6-monooxygenase (NADPH) (ambiguous)
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| Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
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| Sysname |
L-lysine,NADPH:oxygen oxidoreductase (6-hydroxylating)
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| Reaction(IUBMB) |
L-lysine + NADPH + H+ + O2 = N6-hydroxy-L-lysine + NADP+ + H2O [RN: R00448]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
A flavoprotein (FAD). The enzyme from strain EN 222 of Escherichia coli is highly specific for L-lysine; L-ornithine and L-homolysine are, for example, not substrates.
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| History |
EC 1.14.13.59 created 1999, modified 2001, modified 2012
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| Pathway |
| ec00997 | Biosynthesis of various secondary metabolites - part 3 |
| ec01110 | Biosynthesis of secondary metabolites |
| ec01120 | Microbial metabolism in diverse environments |
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| Orthology |
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| Genes |
» show all
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| Reference |
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| Authors |
Plattner HJ, Pfefferle P, Romaguera A, Waschutza S, Diekmann H. |
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| Title |
Isolation and some properties of lysine N6-hydroxylase from Escherichia coli strain EN222. |
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| Journal |
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| Reference |
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| Authors |
Macheroux P, Plattner HJ, Romaguera A, Diekmann H. |
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| Title |
FAD and substrate analogs as probes for lysine N6-hydroxylase from Escherichia coli EN 222. |
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| Journal |
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| Reference |
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| Authors |
Thariath AM, Fatum KL, Valvano MA, Viswanatha T. |
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| Title |
Physico-chemical characterization of a recombinant cytoplasmic form of lysine: N6-hydroxylase. |
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| Journal |
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| Reference |
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| Authors |
de Lorenzo V, Bindereif A, Paw BH, Neilands JB |
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| Title |
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12. |
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| Journal |
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| Sequence |
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| Reference |
5 |
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| Authors |
Marrone, L., Siemann, S., Beecroft, M. and Viswanatha, T. |
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| Title |
Specificity of lysine:N-6-hydroxylase: A hypothesis for a reactive substrate intermediate in the catalytic mechanism. |
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| Journal |
Bioorg Chem 24:401-406 (1996) |
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| Reference |
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| Authors |
Goh CJ, Szczepan EW, Menhart N, Viswanatha T. |
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| Title |
Studies on lysine:N6-hydroxylation by cell-free systems of Aerobacter aerogenes 62-1. |
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| Journal |
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| Other DBs |
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| LinkDB |
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