Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

phenol,NADPH:oxygen oxidoreductase (2-hydroxylating)

phenol + NADPH + H+ + O2 = catechol + NADP+ + H2O [RN:R00815]

R00815;
(other) R03566 R03568
Reaction

phenol [CPD:C00146];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

A flavoprotein (FAD). The enzyme from the fungus Trichosporon cutaneum has a broad substrate specificity, and has been reported to catalyse the hydroxylation of a variety of substituted phenols, such as fluoro-, chloro-, amino- and methyl-phenols and also dihydroxybenzenes. cf. EC 1.14.14.20, phenol 2-monooxygenase (FADH2).

EC 1.14.13.7 created 1972, modified 2011, modified 2016

1  [PMID:14478080]

NAKAGAWA H, TAKEDA Y.

Phenol hydroxylase.

Biochim Biophys Acta 62:423-6 (1962)
DOI:10.1016/0006-3002(62)90275-5

2  [PMID:4146224]

Neujahr HY, Gaal A

Phenol hydroxylase from yeast. Purification and properties of the enzyme from Trichosporon cutaneum.

Eur J Biochem 35:386-400 (1973)
DOI:10.1111/j.1432-1033.1973.tb02851.x

[ag:AAA34202]

3  [PMID:810352]

Neujahr HY, Gaal A.

Phenol hydroxylase from yeast. Sulfhydryl groups in phenol hydroxylase from Trichosporon cutaneum.

Eur J Biochem 58:351-7 (1975)
DOI:10.1111/j.1432-1033.1975.tb02381.x