Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

(4-hydroxyphenyl)ethan-1-one,NADPH:oxygen oxidoreductase (ester-forming)

(4-hydroxyphenyl)ethan-1-one + NADPH + H+ + O2 = 4-hydroxyphenyl acetate + NADP+ + H2O [RN:R06892]

R06892
Reaction

(4-hydroxyphenyl)ethan-1-one [CPD:C10700];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

Contains FAD. The enzyme from Pseudomonas fluorescens ACB catalyses the conversion of a wide range of acetophenone derivatives. Highest activity occurs with compounds bearing an electron-donating substituent at the para position of the aromatic ring [1]. In the absence of substrate, the enzyme can act as an NAD(P)H oxidase (EC 1.6.3.1).

EC 1.14.13.84 created 2004

1  [PMID:11322873]

Kamerbeek NM, Moonen MJ, Van Der Ven JG, Van Berkel WJ, Fraaije MW, Janssen DB

4-Hydroxyacetophenone monooxygenase from Pseudomonas fluorescens ACB. A novel flavoprotein catalyzing Baeyer-Villiger oxidation of aromatic compounds.

Eur J Biochem 268:2547-57 (2001)
DOI:10.1046/j.1432-1327.2001.02137.x

[ag:AAK54073]

2  [PMID:12514023]

Kamerbeek NM, Olsthoorn AJ, Fraaije MW, Janssen DB.

Substrate specificity and enantioselectivity of 4-hydroxyacetophenone monooxygenase.

Appl Environ Microbiol 69:419-26 (2003)
DOI:10.1128/AEM.69.1.419-426.2003

[ag:AAK54073]