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17alpha-hydroxyprogesterone deacetylase;
C-17/C-20 lyase;
17alpha-hydroxyprogesterone acetaldehyde-lyase;
CYP17A1 (gene name);
17alpha-hydroxyprogesterone 17,20-lyase
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
17alpha-hydroxyprogesterone,NADPH---hemoprotein reductase:oxygen oxidoreductase (17alpha-hydroxylating, acetate-releasing)
(1) 17alpha-hydroxyprogesterone + [reduced NADPH---hemoprotein reductase] + O2 = androstenedione + acetate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R08518];
(2) 17alpha-hydroxypregnenolone + [reduced NADPH---hemoprotein reductase] + O2 = 3beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R08517]
R08517 R08518
17alpha-hydroxyprogesterone [CPD:C01176];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
17alpha-hydroxypregnenolone [CPD:C05138]
androstenedione [CPD:C00280];
acetate [CPD:C00033];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
3beta-hydroxyandrost-5-en-17-one [CPD:C01227]
A microsomal cytochrome P-450 (heme-thiolate) protein that catalyses two independent reactions at the same active site - the 17-hydroxylation of pregnenolone and progesterone, which is part of glucocorticoid hormones biosynthesis (EC, and the conversion of the 17-hydroxylated products via a 17,20-lyase reaction to form androstenedione and 3beta-hydroxyandrost-5-en-17-one, leading to sex hormone biosynthesis. The activity of this reaction is dependent on the allosteric interaction of the enzyme with cytochrome b5 without any transfer of electrons from the cytochrome [2,4]. The enzymes from different organisms differ in their substrate specificity. While the enzymes from pig, hamster, and rat accept both 17alpha-hydroxyprogesterone and 17alpha-hydroxypregnenolone, the enzymes from human, bovine, sheep, goat, and bison do not accept the former, and the enzyme from guinea pig does not accept the latter [1].
EC created 1976 as EC, transferred 2016 to EC
ec00140  Steroid hormone biosynthesis
ec01100  Metabolic pathways
K00512  steroid 17alpha-monooxygenase / 17alpha-hydroxyprogesterone deacetylase
HSA: 1586(CYP17A1)
PTR: 450141(CYP17A1)
PPS: 100989182
GGO: 101152080
PON: 100439753
NLE: 100596228
MCC: 678693(CYP17A1)
MCF: 102133235(CYP17)
CSAB: 103216445(CYP17A1)
RRO: 104656530
RBB: 108515083
CJC: 100400572(CYP17A1)
SBQ: 101048584
MMU: 13074(Cyp17a1)
MCAL: 110285327
MPAH: 110334801
RNO: 25146(Cyp17a1)
MUN: 110556807
CGE: 100758683
NGI: 103744072
HGL: 101726496
CCAN: 109691327
OCU: 100346394
TUP: 102472543
CFA: 477807(CYP17A1)
VVP: 112917782
AML: 100476785
UMR: 103681600
UAH: 113251880
ORO: 101384623
ELK: 111145221
FCA: 493967(CYP17A1)
PTG: 102966057
PPAD: 109272832
AJU: 106966901
BTA: 112441470 112444495 281739(CYP17A1)
CHX: 102182376 102182663(CYP17)
OAS: 105601846(CYP17) 493968(CYP17A1)
SSC: 403330(CYP17A1)
CFR: 102519078
CDK: 105095024
BACU: 103007900
LVE: 103081477
OOR: 101283719
DLE: 111172355
PCAD: 102989171
ECB: 100034232(CYP17A1)
EPZ: 103558137
EAI: 106838161
MYB: 102259343
MYD: 102761363
MNA: 107542528
HAI: 109379659
DRO: 112300189
PALE: 102884618
RAY: 107515566
MJV: 108410298
LAV: 100660963
TMU: 101354636
MDO: 100028896
SHR: 100915970
PCW: 110194957
OAA: 100080906
GGA: 425056(CYP17A1)
MGP: 100540331
CJO: 107316091
NMEL: 110399428
APLA: 101792920
ACYG: 106031836
TGU: 100218933
LSR: 110473583
SCAN: 103813852
GFR: 102038147
FAB: 101821194
PHI: 102104333
PMAJ: 107206790
CCAE: 111931270
CCW: 104695563
ETL: 114071768
FPG: 101910363
FCH: 102054501
CLV: 102093575
EGZ: 104125602
NNI: 104022305
ACUN: 113481735
PADL: 103916301
AAM: 106494668
ASN: 102372221
AMJ: 102567971(CYP17A1)
PSS: 102457785
CMY: 102945773
CPIC: 101933089
PVT: 110077624
PBI: 103063631
PMUR: 107290855
TSR: 106541312
PMUA: 114597528
GJA: 107117909
XLA: 100036774(cyp17a1.L)
XTR: 100145650(cyp17a1)
DRE: 399692(cyp17a1) 796487(cyp17a2)
IPU: 100313515(cyp17) 108275468
TRU: 100125766(cyp17a2) 100125798(cyp17a1) 115249663
NCC: 104957534
ONL: 100702216(cyp17a2) 100703469(cyp17)
OLA: 100125816(cyp17) 101170547(cyp17a2)
OTW: 112252939
RTP: 109937010
 » show all
1  [PMID:12693981]
Gilep AA, Estabrook RW, Usanov SA
Molecular cloning and heterologous expression in E. coli of cytochrome P45017alpha. Comparison of structural and functional properties of substrate-specific cytochromes P450 from different species.
Biochemistry (Mosc) 68:86-98 (2003)
2  [PMID:9452426]
Auchus RJ, Lee TC, Miller WL
Cytochrome b5 augments the 17,20-lyase activity of human P450c17 without direct electron transfer.
J Biol Chem 273:3158-65 (1998)
3  [PMID:26668369]
Mak PJ, Gregory MC, Denisov IG, Sligar SG, Kincaid JR
Unveiling the crucial intermediates in androgen production.
Proc Natl Acad Sci U S A 112:15856-61 (2015)
4  [PMID:26587646]
Simonov AN, Holien JK, Yeung JC, Nguyen AD, Corbin CJ, Zheng J, Kuznetsov VL, Auchus RJ, Conley AJ, Bond AM, Parker MW, Rodgers RJ, Martin LL
Mechanistic Scrutiny Identifies a Kinetic Role for Cytochrome b5 Regulation of Human Cytochrome P450c17 (CYP17A1, P450 17A1).
PLoS One 10:e0141252 (2015)
5  [PMID:26976652]
Bhatt MR, Khatri Y, Rodgers RJ, Martin LL.
Role of cytochrome b5 in the modulation of the enzymatic activities of cytochrome P450 17alpha-hydroxylase/17,20-lyase (P450 17A1).
J Steroid Biochem Mol Biol 170:2-18 (2017)
Other DBs
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CAS: 62213-24-5

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