Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

progesterone,reduced-ferredoxin:oxygen oxidoreductase (15beta-hydroxylating)

progesterone + 2 reduced [2Fe-2S] ferredoxin + O2 = 15beta-hydroxyprogesterone + 2 oxidized [2Fe-2S] ferredoxin + H2O [RN:R09587]

R09587

progesterone [CPD:C00410];
reduced [2Fe-2S] ferredoxin [CPD:C22150];
O2 [CPD:C00007]

The enzyme from the bacterium Bacillus megaterium hydroxylates a variety of 3-oxo-Delta4-steroids in position 15beta. Ring A-reduced, aromatic, and 3beta-hydroxy-Delta4-steroids do not serve as substrates [2].

EC 1.14.15.8 created 2010

1  [PMID:109432]

Berg A, Ingelman-Sundberg M, Gustafsson JA

Purification and characterization of cytochrome P-450meg.

J Biol Chem 254:5264-71 (1979)

2  [PMID:177422]

Berg A, Gustafsson JA, Ingelman-Sundberg M

Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium.

J Biol Chem 251:2831-8 (1976)

3  [PMID:15178459]

Lisurek M, Kang MJ, Hartmann RW, Bernhardt R

Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass  spectrometry.

Biochem Biophys Res Commun 319:677-82 (2004)
DOI:10.1016/j.bbrc.2004.05.037

[ag:CAA79985]

4  [PMID:19635596]

Goni G, Zollner A, Lisurek M, Velazquez-Campoy A, Pinto S, Gomez-Moreno C, Hannemann F, Bernhardt R, Medina M

Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368.

Biochim Biophys Acta 1794:1635-42 (2009)
DOI:10.1016/j.bbapap.2009.07.012

5  [PMID:18481342]

Lisurek M, Simgen B, Antes I, Bernhardt R

Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation.

Chembiochem 9:1439-49 (2008)
DOI:10.1002/cbic.200700670

[ag:CAA79985]