Entry |
|
Name |
steroid 15beta-monooxygenase;
cytochrome P-450meg;
cytochrome P450meg;
steroid 15beta-hydroxylase;
CYP106A2;
BmCYP106A2
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Class |
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
|
Sysname |
progesterone,reduced-ferredoxin:oxygen oxidoreductase (15beta-hydroxylating)
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Reaction(IUBMB) |
progesterone + 2 reduced [2Fe-2S] ferredoxin + O2 = 15beta-hydroxyprogesterone + 2 oxidized [2Fe-2S] ferredoxin + H2O [RN: R09587]
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Reaction(KEGG) |
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Substrate |
|
Product |
15beta-hydroxyprogesterone [CPD: C19726];
oxidized [2Fe-2S] ferredoxin [CPD: C22151];
H2O [CPD: C00001]
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Comment |
The enzyme from the bacterium Bacillus megaterium hydroxylates a variety of 3-oxo-Delta4-steroids in position 15beta. Ring A-reduced, aromatic, and 3beta-hydroxy-Delta4-steroids do not serve as substrates [2].
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History |
EC 1.14.15.8 created 2010
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Orthology |
K21114 | steroid 15beta-monooxygenase |
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Genes |
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Reference |
|
Authors |
Berg A, Ingelman-Sundberg M, Gustafsson JA |
Title |
Purification and characterization of cytochrome P-450meg. |
Journal |
J Biol Chem 254:5264-71 (1979) |
Reference |
|
Authors |
Berg A, Gustafsson JA, Ingelman-Sundberg M |
Title |
Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium. |
Journal |
J Biol Chem 251:2831-8 (1976) |
Reference |
|
Authors |
Lisurek M, Kang MJ, Hartmann RW, Bernhardt R |
Title |
Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry. |
Journal |
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Sequence |
|
Reference |
|
Authors |
Goni G, Zollner A, Lisurek M, Velazquez-Campoy A, Pinto S, Gomez-Moreno C, Hannemann F, Bernhardt R, Medina M |
Title |
Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368. |
Journal |
|
Reference |
|
Authors |
Lisurek M, Simgen B, Antes I, Bernhardt R |
Title |
Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation. |
Journal |
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Sequence |
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Other DBs |
ExPASy - ENZYME nomenclature database: | 1.14.15.8 |
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LinkDB |
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