KEGG   ENZYME: 1.14.19.3
Entry
EC 1.14.19.3                Enzyme                                 

Name
acyl-CoA 6-desaturase;
Delta6-desaturase;
Delta6-fatty acyl-CoA desaturase;
Delta6-acyl CoA desaturase;
fatty acid Delta6-desaturase;
fatty acid 6-desaturase;
linoleate desaturase;
linoleic desaturase;
linoleic acid desaturase;
linoleoyl CoA desaturase;
linoleoyl-coenzyme A desaturase;
long-chain fatty acid Delta6-desaturase;
linoleoyl-CoA,hydrogen-donor:oxygen oxidoreductase;
linoleoyl-CoA desaturase;
FADS2 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
acyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (6,7 cis-dehydrogenating)
Reaction(IUBMB)
(1) linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R03814];
(2) alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R07933]
Reaction(KEGG)
Substrate
linoleoyl-CoA [CPD:C02050];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
alpha-linolenoyl-CoA [CPD:C16162]
Product
gamma-linolenoyl-CoA [CPD:C03035];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
stearidonoyl-CoA [CPD:C16163]
Comment
An iron protein. The enzyme introduces a cis double bond at carbon 6 of acyl-CoAs. It is a front-end desaturase, introducing the new double bond between a pre-existing double bond and the carboxyl-end of the fatty acid. The human enzyme has a broad substrate range. It also acts on palmitoyl-CoA, generating sapienoyl-CoA [4], and on (9Z,12Z,15Z,18Z,21Z)-tetracosa-9,12,15,18,21-pentaenoyl-CoA, converting it to (6Z,9Z,12Z,15Z,18Z,21Z)-tetracosa-6,9,12,15,18,21-hexaenoyl-CoA as part of a pathway that produces docosahexaenoate [3]. The enzyme contains a cytochrome b5 domain that is assumed to act in vivo as the electron donor to the active site of the desaturase.
History
EC 1.14.19.3 created 1986 as EC 1.14.99.25, transferred 2000 to EC 1.14.19.3, modified 2015
Pathway
ec00591  Linoleic acid metabolism
ec01040  Biosynthesis of unsaturated fatty acids
ec01100  Metabolic pathways
Orthology
K00508  linoleoyl-CoA desaturase
K10226  acyl-CoA 6-desaturase (Delta-6 desaturase)
Genes
HSA: 9415(FADS2)
PTR: 451244(FADS2)
PPS: 100980611(FADS2)
GGO: 109024898(FADS2)
PON: 100190814(FADS2)
NLE: 100596455(FADS2)
MCC: 719037(FADS2)
MCF: 101926688(FADS2)
CSAB: 103234245(FADS2)
RRO: 104679373(FADS2)
RBB: 108533600(FADS2)
CJC: 100387410(FADS2)
SBQ: 101051429(FADS2)
MMU: 56473(Fads2)
MCAL: 110285898(Fads2)
MPAH: 110322460(Fads2)
RNO: 83512(Fads2)
MUN: 110548092(Fads2)
CGE: 100757464(Fads2)
NGI: 103729125(Fads2)
HGL: 101726663(Fads2)
CCAN: 109695673(Fads2)
OCU: 100352712(FADS2)
TUP: 102475407(FADS2)
CFA: 483792(FADS2)
VVP: 112911087(FADS2)
AML: 100470289(FADS2) 100470779
UMR: 103666104 103678231(FADS2)
UAH: 113246983(FADS2)
ORO: 101364529(FADS2)
FCA: 101085071(FADS2)
PTG: 102968308(FADS2) 102969100
PPAD: 109246554(FADS2) 109261216
AJU: 106986967(FADS2) 106988351
BTA: 521822(FADS2)
BOM: 102275533(FADS2)
BIU: 109554370(FADS2)
BBUB: 102396145 102405983(FADS2)
CHX: 100861212(FADS2) 102171133(FADS2)
OAS: 101108497 101108615(FADS2)
SSC: 444997(FADS2)
CFR: 102506325(FADS2)
CDK: 105091525(FADS2) 105104977
BACU: 103000252(FADS2)
LVE: 103083143(FADS2)
OOR: 101275563(FADS2)
DLE: 111183883(FADS2)
PCAD: 114487991(FADS2)
ECB: 100062709(FADS2)
EPZ: 103543786 103567043(FADS2)
EAI: 106847560(FADS2)
MYB: 102261893(FADS2)
MYD: 102773468(FADS2)
MNA: 107527684(FADS2)
HAI: 109390019(FADS2)
DRO: 112317237(FADS2)
PALE: 102894063(FADS2) 107196578
RAY: 107501589(FADS2)
MJV: 108407763(FADS2)
LAV: 100677680
TMU: 101347025
MDO: 100009895(FADS2)
SHR: 100930034(FADS2)
PCW: 110195307(FADS2)
OAA: 100086288
GGA: 423122(FADS2)
MGP: 100549977
CJO: 107314439(FADS2)
NMEL: 110400735(FADS2)
APLA: 101798758(FADS2)
ACYG: 106033870
TGU: 100222881(FADS2)
LSR: 110484926(FADS2)
SCAN: 103822123(FADS2)
GFR: 102040567(FADS2)
FAB: 101810111(FADS2)
PHI: 102112585(FADS2)
PMAJ: 107205402(FADS2)
CCAE: 111930632
CCW: 104697641
ETL: 114067866
FPG: 101910606(FADS2)
FCH: 102058085(FADS2)
CLV: 102098615(FADS2)
EGZ: 104122147
NNI: 104009393(FADS2)
ACUN: 113481354
PADL: 103925785(FADS2)
AAM: 106490046
ASN: 102382989
AMJ: 102563693(FADS2)
PSS: 102460236(FADS2)
CMY: 102933275(FADS2)
CPIC: 101936434
ACS: 100551818(fads2)
PVT: 110086191(FADS2)
PBI: 103050551(FADS2)
PMUR: 107300095
TSR: 106538426(FADS2)
PMUA: 114600538
GJA: 107108854(FADS2)
XLA: 399058(fads2.S) 446688(fads2.L)
XTR: 100145314(fads2)
NPR: 108794176(FADS2)
DRE: 140615(fads2)
SRX: 107754339
IPU: 100305117(fads2) 108275104
AMEX: 103027972(fads2)
EEE: 113588191(fads2)
LCO: 104937137(fads2)
NCC: 104956187(fads2)
AOCE: 111582148(fads2)
CSEM: 103380276(fads2)
POV: 109623584(fads2)
LCF: 108886851(fads2)
SDU: 111229900(fads2)
SLAL: 111666947(fads2)
HCQ: 109507604(fads2)
BPEC: 110167702(fads2)
SASA: 100136383(fadsd5) 100136441(fadsd6) 100329172(d6fadc) 100337624(d6fad_b) 106584797(D6fad_b)
OTW: 112264870
SALP: 112071110
ELS: 105018065(fads2)
LCM: 102349949(FADS2)
CMK: 103174861(fads2)
RTP: 109937250(fads2)
BFO: 118428221
DPA: 109544444
OBI: 106867675
SHX: MS3_06568
AQU: 100632154
ECOS: EC958_1504
ECC: c1695
ESE: ECSF_1212
ELC: i14_1525
ELD: i02_1525
ELF: LF82_208
ECOJ: P423_06960
EFE: EFER_1726
EEC: EcWSU1_02601(des6)
SRL: SOD_c26990(des1) SOD_c27000(des)
ECA: ECA0116
DKO: I596_2530
PPSE: BN5_0558
TOL: TOL_2040
CVI: CV_1644(desD) CV_2452
BUB: BW23_4705
BSTG: WT74_24150
ADE: Adeh_1021
CCX: COCOR_07436(des6)
HOH: Hoch_5632
SYN: sll0262(desD(des6))
SYZ: MYO_119490(desD(des6))
SYY: SYNGTS_1931(desD)
SYT: SYNGTI_1930(desD)
SYS: SYNPCCN_1929(desD)
SYQ: SYNPCCP_1929(desD)
SYJ: D082_30330(desD)
AMR: AM1_1073
MAR: MAE_48160(desD)
MPK: VL20_3454
MVZ: myaer102_21530(desD)
ARP: NIES39_J03090(desD)
PUV: PUV_10250(des6)
WCH: wcw_0161
FMR: Fuma_00671(desA)
SACI: Sinac_0398
PHE: Phep_0579
SMIZ: 4412673_01383(desA3_1) 4412673_04012(desA3_2)
SDJ: NCTC13534_02135(desA3)
SLI: Slin_4655
FAE: FAES_3358 FAES_4414(desD)
GFO: GFO_1576
FPS: FP0413
FJG: BB050_00859(desA3_1) BB050_02987(desA3_2)
FIN: KQS_11875
MARM: YQ22_03825
CBAL: M667_08640
CBAT: M666_08605
DDO: I597_1302(desA3)
ZGA: ZOBELLIA_1067(des6-2) ZOBELLIA_755(des6-1)
MLT: VC82_2131
WIN: WPG_2001
TMAR: MARIT_2781
AALG: AREALGSMS7_02543(desA3)
MARF: CJ739_552
MESQ: C7H62_0634
EAO: BD94_3599
CHZ: CHSO_0007
SRG: XF24_00844(desA3)
 » show all
Reference
1  [PMID:7212717]
  Authors
Okayasu T, Nagao M, Ishibashi T, Imai Y.
  Title
Purification and partial characterization of linoleoyl-CoA desaturase from rat liver microsomes.
  Journal
Arch Biochem Biophys 206:21-8 (1981)
DOI:10.1016/0003-9861(81)90061-8
Reference
2  [PMID:9867867]
  Authors
Cho HP, Nakamura MT, Clarke SD
  Title
Cloning, expression, and nutritional regulation of the mammalian Delta-6 desaturase.
  Journal
J Biol Chem 274:471-7 (1999)
DOI:10.1074/jbc.274.1.471
  Sequence
[hsa:9415] [mmu:56473]
Reference
3  [PMID:10903473]
  Authors
Sprecher H
  Title
Metabolism of highly unsaturated n-3 and n-6 fatty acids.
  Journal
Biochim Biophys Acta 1486:219-31 (2000)
DOI:10.1016/S1388-1981(00)00077-9
Reference
4  [PMID:12713571]
  Authors
Ge L, Gordon JS, Hsuan C, Stenn K, Prouty SM
  Title
Identification of the delta-6 desaturase of human sebaceous glands: expression and enzyme activity.
  Journal
J Invest Dermatol 120:707-14 (2003)
DOI:10.1046/j.1523-1747.2003.12123.x
Reference
5  [PMID:15769252]
  Authors
Domergue F, Abbadi A, Zahringer U, Moreau H, Heinz E
  Title
In vivo characterization of the first acyl-CoA Delta6-desaturase from a member of the plant kingdom, the microalga Ostreococcus tauri.
  Journal
Biochem J 389:483-90 (2005)
DOI:10.1042/BJ20050111
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.3
IUBMB Enzyme Nomenclature: 1.14.19.3
ExPASy - ENZYME nomenclature database: 1.14.19.3
BRENDA, the Enzyme Database: 1.14.19.3
CAS: 9082-66-0
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