KEGG   ENZYME: 1.14.19.72
Entry
EC 1.14.19.72               Enzyme                                 

Name
(-)-pluviatolide synthase;
CYP719A23 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
(-)-matairesinol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (methylenedioxy-bridge-forming)
Reaction(IUBMB)
(-)-matairesinol + [reduced NADPH---hemoprotein reductase] + O2 = (-)-pluviatolide + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R11222]
Reaction(KEGG)
R11222
Substrate
(-)-matairesinol [CPD:C10682];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(-)-pluviatolide [CPD:C21191];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme from the plants Sinopodophyllum hexandrum and Podophyllum peltatum catalyses the formation of a methylenedioxy-bridge. It is involved in the biosynthesis of podophyllotoxin, a non-alkaloid toxin lignan whose derivatives are important anticancer drugs.
History
EC 1.14.19.72 created 2016 as EC 1.14.21.11, transferred 2018 to EC 1.14.19.72
Pathway
ec00998  Biosynthesis of various secondary metabolites - part 2
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21207  (-)-pluviatolide synthase
Reference
1  [PMID:23161544]
  Authors
Marques JV, Kim KW, Lee C, Costa MA, May GD, Crow JA, Davin LB, Lewis NG
  Title
Next generation sequencing in predicting gene function in podophyllotoxin biosynthesis.
  Journal
J Biol Chem 288:466-79 (2013)
DOI:10.1074/jbc.M112.400689
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.72
IUBMB Enzyme Nomenclature: 1.14.19.72
ExPASy - ENZYME nomenclature database: 1.14.19.72
BRENDA, the Enzyme Database: 1.14.19.72
LinkDB

DBGET integrated database retrieval system