KEGG   ENZYME: 1.14.99.15
Entry
EC 1.14.99.15               Enzyme                                 

Name
4-methoxybenzoate monooxygenase (O-demethylating);
4-methoxybenzoate 4-monooxygenase (O-demethylating);
4-methoxybenzoate O-demethylase;
p-anisic O-demethylase;
piperonylate-4-O-demethylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
4-methoxybenzoate,hydrogen-donor:oxygen oxidoreductase (O-demethylating)
Reaction(IUBMB)
4-methoxybenzoate + reduced acceptor + O2 = 4-hydroxybenzoate + formaldehyde + acceptor + H2O [RN:R01306]
Reaction(KEGG)
R01306
Substrate
4-methoxybenzoate [CPD:C02519];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
4-hydroxybenzoate [CPD:C00156];
formaldehyde [CPD:C00067];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
The bacterial enzyme consists of a ferredoxin-type protein and an iron-sulfur flavoprotein (FMN). Also acts on 4-ethoxybenzoate, N-methyl-4-aminobenzoate and toluate. The fungal enzyme acts best on veratrate.
History
EC 1.14.99.15 created 1972
Pathway
ec00362  Benzoate degradation
Orthology
K22553  4-methoxybenzoate monooxygenase (O-demethylating)
Genes
PPU: PP_1950
BHZ: ACR54_04430
PUD: G9Q38_04420 G9Q38_04600
PIG: EGT29_07245
HYR: BSY239_2681
HYL: LPB072_21880
MAMO: A6B35_30200
AVI: Avi_7683
NGG: RG540_PA03940
BJA: blr1048
BRA: BRADO6810
BBT: BBta_0729
BRS: S23_67580
AOL: S58_06520
RPA: RPA1871
RPB: RPB_3613
RPT: Rpal_2077
MTUN: MTUNDRAET4_0240.1(cyp199a)
SWI: Swit_3269
SPHD: HY78_09210
SSY: SLG_20150
AAY: WYH_02150(bioI)
SALS: SLNWT_0027
SLE: sle_61820(sle_61820)
ART: Arth_1727
PSIM: KR76_01035
MMAR: MODMU_2722
AMQ: AMETH_2741(cypA)
AMYY: YIM_24070(vdh1)
AORI: SD37_05135
PDX: Psed_3265
PSEA: WY02_26855
PSEH: XF36_15935
PAUT: Pdca_32280
SESP: BN6_55130
AMS: AMIS_8190
 » show all
Reference
1  [PMID:188654]
  Authors
Bernhardt FH, Nastainczyk W, Seydewitz V.
  Title
Kinetic studies on a 4-methoxybenzoate O-demethylase from Pseudomonas putida.
  Journal
Eur J Biochem 72:107-15 (1977)
DOI:10.1111/j.1432-1033.1977.tb11230.x
Reference
2  [PMID:25369]
  Authors
Paszczynski A, Trojanowski J.
  Title
An affinity-column procedure for the purification of veratrate O-demethylase from fungi.
  Journal
Microbios 18:111-21 (1977)
Reference
3  [PMID:6273164]
  Authors
Twilfer H, Bernhardt FH, Gersonde K.
  Title
An electron-spin-resonance study on the redox-active centers of the 4-methoxybenzoate monooxygenase from Pseudomonas putida.
  Journal
Eur J Biochem 119:595-602 (1981)
DOI:10.1111/j.1432-1033.1981.tb05649.x
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.15
IUBMB Enzyme Nomenclature: 1.14.99.15
ExPASy - ENZYME nomenclature database: 1.14.99.15
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.99.15
BRENDA, the Enzyme Database: 1.14.99.15
CAS: 37256-78-3
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