KEGG ENZYME: 1.14.99.56

ENZYME: 1.14.99.56

Entry

EC 1.14.99.56               Enzyme

Name

lytic cellulose monooxygenase (C4-dehydrogenating)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous

Sysname

cellulose, hydrogen-donor:oxygen oxidoreductase (D-glucosyl 4-dehydrogenating)

Reaction(IUBMB)

[(1->4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1->4)-beta-D-glucosyl]n-1 + [(1->4)-beta-D-glucosyl]m + acceptor + H2O [RN:R11747]

Reaction(KEGG)

R11747

Substrate

[(1->4)-beta-D-glucosyl]n+m;
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]

Product

4-dehydro-beta-D-glucosyl-[(1->4)-beta-D-glucosyl]n-1 [CPD:C21628];
[(1->4)-beta-D-glucosyl]m;
acceptor [CPD:C00028];
H2O [CPD:C00001]

Comment

This copper-containing enzyme, found in fungi and bacteria, cleaves cellulose in an oxidative manner. The cellulose fragments that are formed contain a 4-dehydro-D-glucose residue at the non-reducing end. Some enzymes also oxidize cellulose at the C-1 position of the reducing end forming a D-glucono-1,5-lactone residue [cf. EC 1.14.99.54, lytic cellulose monooxygenase (C1-hydroxylating)].

History

EC 1.14.99.56 created 2017

Orthology

K22032

lytic cellulose monooxygenase (C4-dehydrogenating)

K22033

lytic cellulose monooxygenase (C4-dehydrogenating)

Genes

NCR:	NCU01050(gh61-4)

NTE:	NEUTE1DRAFT16830(NEUTE1DRAFT_16830) NEUTE1DRAFT98179(NEUTE1DRAFT_98179)

SMP:	SMAC_02729

PAN:	PODANSg8289

PBEL:

QC761_204860

PPSD:

QC762_204860

PPSP:

QC763_204860

PPSA:

QC764_204860

MTM:	MYCTH_100518 MYCTH_55803

CTHR:

CTHT_0068920

MGR:	MGG_07686 MGG_09709

PPEI:

PpBr36_02492 PpBr36_10121

PGRI:

PgNI_11917

TMN:	UCRPA7_2782

VAL:	VDBG_06653

VDA:	VDAG_05708

CFJ:	CFIO01_08296 CFIO01_11829

CLUP:

CLUP02_06867

CHIG:

CH63R_05053

SAPO:

SAPIO_CDS0715 SAPIO_CDS2413 SAPIO_CDS5581 SAPIO_CDS8558

ELA:	UCREL1_6880

PFY:	PFICI_04777

PSCO:

LY89DRAFT_781244

GLZ:	GLAREA_03141

PNO:	SNOG_00131

PTE:

PTT_19298

BZE:	COCCADRAFT_111217

BSC:	COCSADRAFT_140445

BOR:	COCMIDRAFT_8905

AALT:

CC77DRAFT_560365 CC77DRAFT_930851

ARAB:

EKO05_0003920 EKO05_0009977

NPA:	UCRNP2_205

TVS:	TRAVEDRAFT_36998 TRAVEDRAFT_45192

DSQ:	DICSQDRAFT_106787 DICSQDRAFT_108020

SHS:	STEHIDRAFT_122287

HIR:	HETIRDRAFT_173373(gh61.7) HETIRDRAFT_58346(GH61E)

PSQ:	PUNSTDRAFT_116524

ADL:	AURDEDRAFT_167539

GTR:	GLOTRDRAFT_103762

PCUB:

JR316_0007183

MPR:	MPER_09241

MRR:	Moror_12494

E1B28_002013

SCM:	SCHCO_02634027(SCHCODRAFT_02634027)

SCO:	SCO0643(SCF91.03c)

SCB:	SCAB_89741

SSX:	SACTE_6428

SVL:	Strvi_1641

SHY:

SHJG_4189

SHO:	SHJGH_3954

SRC:	M271_17240 M271_39590

SMAL:

SMALA_1378 SMALA_5277

SSOI:

I1A49_10405 I1A49_30095

SFA:

Sfla_0248

SBH:	SBI_03830 SBI_06957

STRP:

F750_6768

SLV:	SLIV_34725

SGU:	SGLAU_17860 SGLAU_31800 SGLAU_31955

SCW:	TU94_28680 TU94_31990

SXI:	SXIM_50050

STRC:

AA958_22300 AA958_30715

SAMB:

SAM23877_2400 SAM23877_6899

SPRI:

SPRI_6276

SRW:	TUE45_01353 TUE45_03305

STRF:

ASR50_03495

SLE:	sle_03840(sle_03840) sle_04310(sle_04310)

STSI:

A4E84_01725

SPHW:

NFX46_19840

SLS:	SLINC_8348

STRD:

NI25_01805 NI25_18230

SAUO:

BV401_10725 BV401_30210

SSIA:

A7J05_35945

SLAU:

SLA_5870

SGE:	DWG14_00994(cbpD_1)

SAST:

CD934_01630 CD934_32860

SNK:	CP967_00560 CP967_04595

SHAW:

CEB94_02190 CEB94_04840

SFUG:

CNQ36_18300

SPAC:

B1H29_02630

SCOE:

CP976_40950

SHAR:

HUT13_25305

SVD:	CP969_05140

SPRA:

CP972_02775 CP972_30155

SROI:

IAG44_00520

SGJ:	IAG43_29130

SACT:

DMT42_00915

SMAO:

CAG99_00455 CAG99_05110 CAG99_14445 CAG99_21460

SDEC:

L3078_39675

SAKB:

K1J60_04150

SCAE:

IHE65_05070

SDRZ:

NEH16_30490

STEE:

F3L20_20740

SANT:

QR300_35060

SJN:	RI060_41515

SKG:	KJK29_38315

SCOA:

QU709_40920

XCE:

Xcel_2244

IDO:	I598_1144(celB)

CFL:	Cfla_0172 Cfla_0316

CFI:

Celf_0270

CGA:	Celgi_1891

CEZ:	CBP52_03880

CELZ:

E5225_16280 E5225_16290 E5225_17100

CEJ:	GC089_17335 GC089_17345 GC089_18000

CELH:

GXP71_17785

CIRA:

LFM56_16650 LFM56_17160

CPAL:

F1D97_10705

CDON:

KKR89_00820 KKR89_01720

CWAN:

KG103_00785 KG103_01815

CFEN:

KG102_17745

CWN:	NP075_00810

CCHE:

NP064_16190

CXIE:

NP048_01015 NP048_01900

TFU:

Tfu_1268

THAO:

NI17_000695 NI17_006675

TALX:

FOF52_00135 FOF52_03340

NDA:

Ndas_3518

NAKE:

KGD83_07610

NCG:	KGD84_07090

STRR:

EKD16_04575 EKD16_19220

SNAH:

OUQ99_06380

AMAZ:

LUW76_21440

NOA:	BKM31_31445

NOW:	GBF35_12675

NCX:	Nocox_11855(gbpA2)

NGN:	LCN96_13775

NFS:	OIE67_33325

AMD:	AMED_1406 AMED_3114(cpbD)

AMN:	RAM_07125 RAM_15835

AMM:	AMES_1397 AMES_3080(cpbD)

AMZ:	B737_1398 B737_3081(cpbD)

AAB:	A4R43_03160 A4R43_06485

AMYY:

YIM_08095 YIM_19840(gbpA3)

AORI:

SD37_18685

APRT:

MUY14_19995 MUY14_20685

APRE:

CNX65_12550 CNX65_23370

AMI:	Amir_2475 Amir_4693

SSYI:

EKG83_12625 EKG83_22020 EKG83_22455 EKG83_28575 EKG83_41375

KBU:	Q4V64_49245

LED:	BBK82_00475

AHM:	TL08_13345 TL08_13515

ACTI:

UA75_14350 UA75_18525

ACAD:

UA74_14260 UA74_18035

AHG:	AHOG_13415 AHOG_16015(gbpA3)

ACTA:

C1701_02345

ACTU:

Actkin_00625 Actkin_02364 Actkin_03225

CROS:

N8J89_17145

UME:	RM788_10360 RM788_46605

MAU:	Micau_1630 Micau_3953

MIL:	ML5_1889 ML5_4468

MICB:

MicB006_1654 MicB006_3015

MTUA:

CSH63_05960 CSH63_16130

MICH:

FJK98_08510 FJK98_21040

MTEM:

GCE86_02340

MCAB:

HXZ27_09425 HXZ27_10185 HXZ27_30320

VMA:	VAB18032_09980 VAB18032_12745

MCRA:

ID554_04010 ID554_30580

VER:	HUT12_08995 HUT12_13925 HUT12_14465

MENO:

Jiend_03680 Jiend_11450 Jiend_27360

MCHL:

PVK74_10130 PVK74_29370

MFEU:

H1D33_14960

MPRN:

Q3V37_30005

MHAW:

RMN56_03205

AMS:	AMIS_32650 AMIS_36720

ACTN:

L083_3734 L083_4711

AFS:

AFR_17880

AIH:	Aiant_69350

ACTR:

Asp14428_00870

ACTL:

L3i22_025790

ASIC:

Q0Z83_034870 Q0Z83_059600

AOU:	ACTOB_002363

PLK:	CIK06_01520

PLAB:

C6361_01330 C6361_04905 C6361_07730 C6361_07755 C6361_21720

PLAT:

C6W10_01435 C6W10_04320 C6W10_04345 C6W10_16680 C6W10_20320

PFLA:

Pflav_032030 Pflav_084660

PSUU:

Psuf_047550 Psuf_074430(cpbD_2)

PRY:	Prubr_28570

CATI:

CS0771_45810 CS0771_70230

DVC:	Dvina_13525

DROS:

Drose_21645

DAUR:

Daura_39410

DMAT:

Dmats_13075

NHY:	JQS43_22765

CCAZ:

COUCH_23110

GLY:	K3N28_06270 K3N28_17545 K3N28_17550

AEY:	CDG81_08375

» show all

Reference

1 [PMID:22188218]

Authors

Beeson WT, Phillips CM, Cate JH, Marletta MA

Title

Oxidative cleavage of cellulose by fungal copper-dependent polysaccharide monooxygenases.

Journal

J Am Chem Soc 134:890-2 (2012)
DOI:10.1021/ja210657t

Sequence

[ncr:NCU01050]

Reference

2 [PMID:22578542]

Authors

Li X, Beeson WT 4th, Phillips CM, Marletta MA, Cate JH

Title

Structural basis for substrate targeting and catalysis by fungal polysaccharide monooxygenases.

Journal

Structure 20:1051-61 (2012)
DOI:10.1016/j.str.2012.04.002

Sequence

[ncr:NCU01050]

Reference

3 [PMID:24912171]

Authors

Forsberg Z, Mackenzie AK, Sorlie M, Rohr AK, Helland R, Arvai AS, Vaaje-Kolstad G, Eijsink VG

Title

Structural and functional characterization of a conserved pair of bacterial cellulose-oxidizing lytic polysaccharide monooxygenases.

Journal

Proc Natl Acad Sci U S A 111:8446-51 (2014)
DOI:10.1073/pnas.1402771111

Sequence

[sco:SCO0643]

Reference

4 [PMID:26178376]

Authors

Borisova AS, Isaksen T, Dimarogona M, Kognole AA, Mathiesen G, Varnai A, Rohr AK, Payne CM, Sorlie M, Sandgren M, Eijsink VG

Title

Structural and Functional Characterization of a Lytic Polysaccharide Monooxygenase with Broad Substrate Specificity.

Journal

J Biol Chem 290:22955-69 (2015)
DOI:10.1074/jbc.M115.660183

Sequence

[ncr:NCU02916]

Reference

5 [PMID:27213015]

Authors

Patel I, Kracher D, Ma S, Garajova S, Haon M, Faulds CB, Berrin JG, Ludwig R, Record E

Title

Salt-responsive lytic polysaccharide monooxygenases from the mangrove fungus Pestalotiopsis sp. NCi6.

Journal

Biotechnol Biofuels 9:108 (2016)
DOI:10.1186/s13068-016-0520-3

Other DBs

ExplorEnz - The Enzyme Database:

1.14.99.56

IUBMB Enzyme Nomenclature:

1.14.99.56

ExPASy - ENZYME nomenclature database:

1.14.99.56

BRENDA, the Enzyme Database:

1.14.99.56

LinkDB

DBGET integrated database retrieval system