KEGG   ENZYME: 1.14.99.66
Entry
EC 1.14.99.66               Enzyme                                 

Name
[histone H3]-N6,N6-dimethyl-L-lysine4 FAD-dependent demethylase;
KDM1 (gene name);
LSD1 (gene name);
lysine-specific histone demethylase 1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
[histone H3]-N6,N6-dimethyl-L-lysine4:acceptor oxidoreductase (demethylating)
Reaction(IUBMB)
a [histone H3]-N6,N6-dimethyl-L-lysine4 + 2 acceptor + 2 H2O = a [histone H3]-L-lysine4 + 2 formaldehyde + 2 reduced acceptor (overall reaction) [RN:R12462];
(1a) a [histone H3]-N6,N6-dimethyl-L-lysine4 + acceptor + H2O = a [histone H3]-N6-methyl-L-lysine4 + formaldehyde + reduced acceptor [RN:R12463];
(1b) a [histone H3]-N6-methyl-L-lysine4 + acceptor + H2O = a [histone H3]-L-lysine4 + formaldehyde + reduced acceptor [RN:R12464]
Reaction(KEGG)
Substrate
[histone H3]-N6,N6-dimethyl-L-lysine4;
acceptor [CPD:C00028];
H2O [CPD:C00001];
[histone H3]-N6-methyl-L-lysine4
Product
[histone H3]-L-lysine4;
formaldehyde [CPD:C00067];
reduced acceptor [CPD:C00030];
[histone H3]-N6-methyl-L-lysine4
Comment
The enzyme specifically removes methyl groups from mono- and dimethylated lysine4 of histone 3. During the reaction the substrate is oxidized by the FAD cofactor of the enzyme to generate the corresponding imine, which is subsequently hydrolysed in the form of formaldehyde.The enzyme is similar to flavin amine oxidases, and differs from all other known histone lysine demethylases, which are iron(II)- and 2-oxoglutarate-dependent dioxygenases. The physiological electron acceptor is not known with certainty. In vitro the enzyme can use oxygen, which is reduced to hydrogen peroxide, but generation of hydrogen peroxide in the chromatin environment is unlikely as it will result in oxidative damage of DNA.
History
EC 1.14.99.66 created 2019
Orthology
K11450  [histone H3]-N6,N6-dimethyl-L-lysine4 FAD-dependent demethylase
Genes
HSA: 23028(KDM1A)
PTR: 456614(KDM1A)
PPS: 100995039(KDM1A)
GGO: 101144258(KDM1A)
PON: 100171870(KDM1A)
NLE: 100579631(KDM1A)
MCC: 718609(KDM1A)
MCF: 102135743(KDM1A)
CSAB: 103225404(KDM1A)
RRO: 104663232(KDM1A)
RBB: 108538436(KDM1A)
CJC: 100406478(KDM1A)
SBQ: 101034653(KDM1A)
MMU: 99982(Kdm1a)
MCAL: 110292704(Kdm1a)
MPAH: 110322979(Kdm1a)
RNO: 500569(Kdm1a)
MUN: 110545399(Kdm1a)
CGE: 100770721(Kdm1a)
NGI: 103746403(Kdm1a)
HGL: 101706376(Kdm1a)
CCAN: 109681855(Kdm1a)
OCU: 100358443(KDM1A)
TUP: 102491246(KDM1A)
CFA: 478193(KDM1A)
VVP: 112930162(KDM1A)
AML: 100469590(KDM1A)
UMR: 103666569(KDM1A)
UAH: 113268534(KDM1A)
ORO: 101371349(KDM1A)
ELK: 111154639
FCA: 101096583(KDM1A)
PTG: 102952813(KDM1A)
PPAD: 109274289(KDM1A)
AJU: 106977650(KDM1A)
BTA: 532997(KDM1A)
BOM: 102287646(KDM1A)
BIU: 109574017(KDM1A)
BBUB: 102396562(KDM1A)
CHX: 102185879(KDM1A)
OAS: 101121409(KDM1A)
SSC: 100127157(KDM1A)
CFR: 102517288(KDM1A)
CDK: 105088880(KDM1A)
BACU: 103012479(KDM1A)
LVE: 103084888(KDM1A)
OOR: 101273117(KDM1A)
DLE: 111163462(KDM1A)
PCAD: 102995575(KDM1A)
ECB: 100071646(KDM1A)
EPZ: 103551290(KDM1A)
EAI: 106839994(KDM1A)
MYB: 102261213(KDM1A)
MYD: 102754604(KDM1A)
MNA: 107538326(KDM1A)
HAI: 109375888(KDM1A)
DRO: 112299203(KDM1A)
PALE: 102887129(KDM1A)
RAY: 107513066(KDM1A)
MJV: 108391096(KDM1A)
LAV: 100663912(KDM1A)
TMU: 101347919
MDO: 100025161(KDM1A)
SHR: 100923421(KDM1A)
PCW: 110206041(KDM1A)
OAA: 100086658(KDM1A)
GGA: 419571(KDM1A)
MGP: 100544521(KDM1A)
CJO: 107323904(KDM1A)
NMEL: 110387355(KDM1A)
APLA: 101803659(KDM1A)
ACYG: 106046571(KDM1A)
TGU: 100221744(KDM1A)
LSR: 110484269(KDM1A)
SCAN: 103822578(KDM1A)
GFR: 102041827(KDM1A)
FAB: 101810697(KDM1A)
PHI: 102109140(KDM1A)
PMAJ: 107214056(KDM1A)
CCAE: 111939020(KDM1A)
CCW: 104697964(KDM1A)
ETL: 114061578(KDM1A)
FPG: 101923221(KDM1A)
FCH: 102058829(KDM1A)
CLV: 102088529(KDM1A)
EGZ: 104132548(KDM1A)
NNI: 104015516(KDM1A)
ACUN: 113488263(KDM1A)
PADL: 103925455(KDM1A)
AAM: 106484726(KDM1A)
ASN: 102375236(KDM1A)
AMJ: 102563192(KDM1A)
PSS: 102460305(KDM1A)
CMY: 102931629(KDM1A)
CPIC: 101940420(KDM1A)
ACS: 100557968(kdm1a)
PVT: 110089669(KDM1A)
PBI: 103067389(KDM1A)
PMUR: 107288027(KDM1A)
TSR: 106540788(KDM1A)
PMUA: 114603591(KDM1A)
GJA: 107116662(KDM1A)
XLA: 100127344(kdm1a.S) 108708259(kdm1a.L)
XTR: 100216250(kdm1a)
NPR: 108788279(KDM1A)
DRE: 558450(kdm1a)
SRX: 107711588
SGH: 107582487
CCAR: 109048821(kdm1a)
IPU: 108269802(kdm1a)
PHYP: 113533951(kdm1a)
AMEX: 103023119(kdm1a)
EEE: 113584671(kdm1a)
TRU: 101073663(kdm1a)
LCO: 104931691(kdm1a)
NCC: 104968042(kdm1a)
MZE: 101466759(kdm1a)
ONL: 100702505(kdm1a)
OLA: 101172293(kdm1a)
XMA: 102231844(kdm1a)
XCO: 114134181(kdm1a)
PRET: 103459946(kdm1a)
CVG: 107084713(kdm1a)
NFU: 107392201(kdm1a)
KMR: 108236889(kdm1a)
ALIM: 106533898
AOCE: 111573331(kdm1a)
CSEM: 103378492(kdm1a)
POV: 109636394(kdm1a)
LCF: 108874888
SDU: 111226160(kdm1a)
SLAL: 111655910(kdm1a)
HCQ: 109526830(kdm1a)
BPEC: 110161360(kdm1a)
MALB: 109955009(kdm1a)
ELS: 105016064(kdm1a)
SFM: 108922806(kdm1a)
PKI: 111854724(kdm1a)
LCM: 102363978(KDM1A)
CMK: 103179609(kdm1a)
RTP: 109926736(kdm1a)
BFO: 118422441
CIN: 100187100
SPU: 575875
APLC: 110990427
SKO: 100373836
DME: Dmel_CG17149(Su(var)3-3)
DER: 6545751
DSE: 6616257
DSI: Dsimw501_GD14861(Dsim_GD14861)
DAN: 6507938
DSR: 110178555
DPE: 6596031
DMN: 108152784
DWI: 6638853
DAZ: 108613120
DNV: 108651092
DHE: 111600951
DVI: 6624613
MDE: 101897898
LCQ: 111681191
AAG: 5574767
AALB: 109422398
AME: 726469
BIM: 100740140
BTER: 100647583
CCAL: 108622958
OBB: 114871968
SOC: 105200954
MPHA: 105832792
AEC: 105143630
ACEP: 105622905
PBAR: 105433548
VEM: 105564973
HST: 105185849
DQU: 106751689
CFO: 105253997
LHU: 105674077
PGC: 109854993
OBO: 105282940
PCF: 106786794
NVI: 100678665
CSOL: 105360759
MDL: 103580293
TCA: 103315031
DPA: 109547044
ATD: 109597000
NVL: 108560657
BMOR: 101739058
BMAN: 114240311
PMAC: 106719592
PRAP: 110998007
HAW: 110370308
TNL: 113497903
PXY: 105386718(bursa)
API: 100168654
DNX: 107174085
AGS: 114126851
RMD: 113549832
CLEC: 106672331
FCD: 110853963
PVM: 113807228
TUT: 107368558
DPTE: 113789474
CSCU: 111638896
PTEP: 107446922
PCAN: 112561001
CRG: 105346267
MYI: 110454266
OBI: 106869849
LAK: 106170923
SHX: MS3_02208
EGL: EGR_08887
NVE: 5512813
EPA: 110237306
ADF: 107335615
AMIL: 114968998
PDAM: 113675980
SPIS: 111342045
DGT: 114533681
AQU: 100631436
OSA: 4344644
DOSA: Os08t0143400-01(Os08g0143400)
BDI: 100835742
ATS: 109781192(LOC109781192)
PDA: 103724047
EGU: 105045418
MUS: 103986351
DCT: 110107040
PEQ: 110039551
AOF: 109844854
 » show all
Reference
1  [PMID:15811342]
  Authors
Forneris F, Binda C, Vanoni MA, Mattevi A, Battaglioli E
  Title
Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process.
  Journal
FEBS Lett 579:2203-7 (2005)
DOI:10.1016/j.febslet.2005.03.015
  Sequence
[hsa:23028]
Reference
2  [PMID:19624733]
  Authors
Forneris F, Battaglioli E, Mattevi A, Binda C
  Title
New roles of flavoproteins in molecular cell biology: histone demethylase LSD1 and chromatin.
  Journal
FEBS J 276:4304-12 (2009)
DOI:10.1111/j.1742-4658.2009.07142.x
  Sequence
[hsa:23028]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.66
IUBMB Enzyme Nomenclature: 1.14.99.66
ExPASy - ENZYME nomenclature database: 1.14.99.66
BRENDA, the Enzyme Database: 1.14.99.66
LinkDB

DBGET integrated database retrieval system