KEGG ENZYME: 1.21.3.11

ENZYME: 1.21.3.11

Entry

EC 1.21.3.11                Enzyme

Name

precondylocarpine acetate synthase;
PAS (gene name)

Class

Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With oxygen as acceptor

Sysname

stemmadenine acetate:oxygen oxidoreductase

Reaction(IUBMB)

stemmadenine acetate + O2 = precondylocarpine acetate + H2O2 [RN:R13344]

Reaction(KEGG)

R13344

Substrate

stemmadenine acetate [CPD:C22933];
O2 [CPD:C00007]

Product

precondylocarpine acetate [CPD:C22934];
H2O2 [CPD:C00027]

Comment

The enzyme, characterized from the plant Catharanthus roseus (Madagascar periwinkle), participates in a pathway that leads to the production of a number of monoterpene alkaloids, as well as the bisindole alkaloids vinblastine and vincristine, which are used as anticancer drugs. The enzyme is similar to berberine bridge enzymes such as EC 1.21.3.3, reticuline oxidase, and requires an FAD cofactor.

History

EC 1.21.3.11 created 2024

Pathway

ec00901

Indole alkaloid biosynthesis

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K27792

precondylocarpine acetate synthase

Reference

1 [PMID:29724909]

Authors

Caputi L, Franke J, Farrow SC, Chung K, Payne RME, Nguyen TD, Dang TT, Soares Teto Carqueijeiro I, Koudounas K, Duge de Bernonville T, Ameyaw B, Jones DM, Vieira IJC, Courdavault V, O'Connor SE

Title

Missing enzymes in the biosynthesis of the anticancer drug vinblastine in Madagascar periwinkle.

Journal

Science 360:1235-1239 (2018)
DOI:10.1126/science.aat4100

Sequence

[ag:AWJ76616]

Other DBs

ExplorEnz - The Enzyme Database:

1.21.3.11

IUBMB Enzyme Nomenclature:

1.21.3.11

ExPASy - ENZYME nomenclature database:

1.21.3.11

BRENDA, the Enzyme Database:

1.21.3.11

LinkDB

DBGET integrated database retrieval system