Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With unknown physiological acceptors
BRITE hierarchy

acceptor:trichloroethene oxidoreductase (chlorinating)

trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor [RN:R05753]

R05753;
(other) R05499 R05500 R05501
Reaction

trichloroethene [CPD:C06790];
chloride [CPD:C00698];
acceptor [CPD:C00028]

This enzyme allows the common pollutant tetrachloroethene to support bacterial growth and is responsible for disposal of a number of chlorinated hydrocarbons. The reaction occurs in the reverse direction. The enzyme also reduces trichloroethene to dichloroethene. Although the physiological reductant is unknown, the supply of reductant in some organisms involves menaquinol, which is reduced by molecular hydrogen via the action of EC 1.12.5.1, hydrogen:quinone oxidoreductase. The enzyme contains a corrinoid and two iron-sulfur clusters. Methylviologen can act as electron donor in vitro.

EC 1.21.99.5 created 2001 as EC 1.97.1.8, transferred 2017 to EC 1.21.99.5

1

Holliger, C, Wohlfarth, G. and Diekert, G.

Reductive dechlorination in the energy metabolism of anaerobic bacteria.

FEMS Microbiol Rev 22:383-398 (1998)

2

Glod, G., Angst, W., Holliger, C. and Schwarzenbach, R.P.

Corrinoid-mediated reduction of tetrachloroethene, trichloroethene, and trichlorofluoroethene in homogeneous aqueous solution: Reaction kinetics and reaction mechanisms.

Environ Sci Technol 31:253-260 (1997)

3  [PMID:8663199]

Neumann A, Wohlfarth G, Diekert G.

Purification and characterization of tetrachloroethene reductive dehalogenase from Dehalospirillum multivorans.

J Biol Chem 271:16515-9 (1996)
DOI:10.1074/jbc.271.28.16515

4  [PMID:9224702]

Schumacher W, Holliger C, Zehnder AJ, Hagen WR.

Redox chemistry of cobalamin and iron-sulfur cofactors in the tetrachloroethene reductase of Dehalobacter restrictus.

FEBS Lett 409:421-5 (1997)
DOI:10.1016/S0014-5793(97)00520-6

5  [PMID:8636034]

Schumacher W, Holliger C.

The proton/electron ration of the menaquinone-dependent electron transport from dihydrogen to tetrachloroethene in "Dehalobacter restrictus".

J Bacteriol 178:2328-33 (1996)
DOI:10.1128/JB.178.8.2328-2333.1996