KEGG   ENZYME: 1.5.98.2
Entry
EC 1.5.98.2                 Enzyme                                 
Name
5,10-methylenetetrahydromethanopterin reductase;
5,10-methylenetetrahydromethanopterin cyclohydrolase;
N5,N10-methylenetetrahydromethanopterin reductase;
methylene-H4MPT reductase;
coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase;
N5,N10-methylenetetrahydromethanopterin:coenzyme-F420 oxidoreductase
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With other, known, physiological acceptors
Sysname
5-methyltetrahydromethanopterin:coenzyme-F420 oxidoreductase
Reaction(IUBMB)
5-methyltetrahydromethanopterin + oxidized coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420 [RN:R04464]
Reaction(KEGG)
R04464
Substrate
5-methyltetrahydromethanopterin [CPD:C04488];
oxidized coenzyme F420 [CPD:C00876]
Product
5,10-methylenetetrahydromethanopterin [CPD:C04377];
reduced coenzyme F420 [CPD:C01080]
Comment
Catalyses an intermediate step in methanogenesis from CO2 and H2 in methanogenic archaea.
History
EC 1.5.98.2 created 2000 as EC 1.5.99.11, modified 2004, transferred to EC 1.5.98.2 2014
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00320  5,10-methylenetetrahydromethanopterin reductase
Genes
ZALAZF00_03740
HALCEY643_08545
KIMG3T16_19900
PSPWBJG93_33595
DOEDENOEST_1262
BICLMTR13_24555 LMTR13_24680
RPBRPB_4412 RPB_4431
NREBES08_26800
SWISwit_0724
SPHDHY78_07885 HY78_23075
RDICMV14_12235
SPKCKC8_06245
SCHSphch_1884
SYBTZ53_18460
SBDATN00_17390 ATN00_17485
SPMIK663_17326 K663_17801
SPHTK426_23754 K426_23849
SHYDCJD35_15390 CJD35_15495
SUFLFIL70_06830 FIL70_06930 FIL70_11560
CMANA9D14_14795
KHAIFJ82_10875
MPAMAP_0675
MAOMAP4_3189
MAVIRC58_15805
MAVURE97_15835
MAVMAV_1930 MAV_5061
MITOCO_13230 OCO_25030 OCO_39800
MIAOCU_07100 OCU_13700 OCU_24900 OCU_39700
MIDMIP_00259 MIP_03465 MIP_05993
MYOOEM_23460
MCHIAN480_04710 AN480_12585 AN480_20895
MIROCQ_23580 OCQ_28210 OCQ_29400 OCQ_40880
MMALCKJ54_11345
MSAMycsm_03546 Mycsm_04592 Mycsm_06787 Mycsm_06803
MMCMmcs_3824
MKMMkms_3898
MJLMjls_3810 Mjls_4047
MMMW7S_08445 W7S_12080 W7S_19830
MYVG155_01495 G155_03345 G155_28015
MYEAB431_15950
MGOAFA91_08815 AFA91_27215 AFA91_32745
MDXBTO20_10285 BTO20_23080 BTO20_34590
MFJMFLOJ_08070 MFLOJ_18720 MFLOJ_23560 MFLOJ_42820 MFLOJ_56420
MSTOMSTO_09290 MSTO_20260 MSTO_27520 MSTO_46930
MSIMMSIM_14700
MGROFZ046_08030 FZ046_16835
MXEMYXE_08020(hmd)
MPAGC0J29_20060
MGORH0P51_10265
MCOOMCOO_10090
MSEOMSEO_32410
MHEKJMUB5695_00830(fgd)
MBRDMBRA_11340
MKRMKOR_28420
MDFK0O62_17150
MMAMK3U93_09515
MHERK3U94_10745
MSENK3U95_03240
MPAEK0O64_15015
MSMMSMEG_2103 MSMEG_2456 MSMEG_3445
MSGMSMEI_2056 MSMEI_2395 MSMEI_3365
MSBLJ00_10475 LJ00_12225 LJ00_17140
MSNLI99_10475 LI99_12225 LI99_17145
MSHLI98_10475 LI98_12230 LI98_17150
MVAMvan_0261 Mvan_4259
MGIMflv_0410
MSPMspyr1_03440
MCBMycch_0231
MNED174_03035
MYNMyAD_02965
MFTXA26_03220 XA26_06010 XA26_56620
MPHLMPHLCCUG_03824
MVQMYVA_0266 MYVA_2357 MYVA_4061
MLLB1R94_12750 B1R94_16695
MRHMycrhN_3896
MTHN4412656_00262 4412656_03260(fgd1_4)
MHASMHAS_00010(fgd1_1)
MDUMDUV_19340 MDUV_30540 MDUV_40590
MCHTMCHIJ_11280(hmd_2)
MAUUNCTC10437_00266 NCTC10437_02307(fgd1_5) NCTC10437_04003 NCTC10437_05699
MMAGMMAD_05430 MMAD_16820 MMAD_39820
MMORMMOR_05480 MMOR_22830 MMOR_28450(hmd) MMOR_42210
MAICMAIC_27490
MALVMALV_21310 MALV_28120 MALV_30900 MALV_32630
MTYMTOK_12730 MTOK_26490 MTOK_30960
MPSCMPSYJ_06270
MARZMARA_16780 MARA_35350 MARA_40190
MGADMGAD_45740 MGAD_56050
MHEVMHEL_32200 MHEL_51230
MANYMANY_24330(hmd) MANY_41960
MAUBMAUB_13640 MAUB_36340 MAUB_37910
MPOFMPOR_08040 MPOR_27800
MPHUMPHO_32370 MPHO_33950
MMUCC1S78_001170 C1S78_001810 C1S78_018685
MMATMMAGJ_54430
MBOKMBOE_17960 MBOE_50810
MFGK6L26_28525
MSEIMSEDJ_16290
MTER4434518_02913 4434518_02982
MMINMMIN_35190
MHIBMHIB_00990(hmd)
NBRO3I_022250
NNONONO_c47850 NONO_c58860
NSLBOX37_14070
NOZDMB37_02885
NWLNWFMUON74_53980 NWFMUON74_57790
NTCKHQ06_23305
NYALTV02_02545
ROAPd630_LPD12069
RRT4535765_00819
RKOJWS14_25785 JWS14_43070 JWS14_45920
GBRGbro_3836
GPOGPOL_c36980
GORKTR9_3774
GOQACH46_18365
GTABCM27_19765
GOCCXX93_05420
GRUGCWB2_11310(fgd4) GCWB2_12090(ssuD) GCWB2_19375
GOMD7316_01986 D7316_02156(fgd2_4)
GAVC5O27_08760
GODGKZ92_18305 GKZ92_19915
GAMGII34_07275
GJIH1R19_18105
DIZCT688_02325
DPCA6048_02280
SGBWQO_00840
SCBSCAB_88981
SSXSACTE_5139
SVLStrvi_3235 Strvi_5584
SRCM271_09195
SMALSMALA_8438
SFISFUL_290
SALUDC74_6554
SALLSAZ_34125
SCWTU94_30975
SXISXIM_17420
STRCAA958_24575
SPRISPRI_3761 SPRI_6783
SLEsle_06190(sle_06190) sle_10720(sle_10720)
SCLFBB341_28080
SLSSLINC_0648 SLINC_2815
SNRSNOUR_24160
SVUB1H20_00460
SGEDWG14_02663(fgd2_1)
SQZFQU76_28675
SCYAEJ357_46330
SASTCD934_00975
SPADDVK44_11360
SFYGFH48_04670
SBYH7H31_03885
SPHVF9278_44540
STSUB7R87_28850
SCOECP976_04140
SBATG4Z16_14350
SSPBCP982_00140
SHARHUT13_11085
SFEUIM697_21740
SVDCP969_02205
SCALI6J39_00585
STRIC7M71_027195 C7M71_029915
YIALO772_01055
MIOAOA12_21255
MCWA8L33_00440
MLVCVS47_00302 CVS47_02477 CVS47_02481
RTNA6122_0692
RRYC1O28_02305
RIAC7V51_08830
RFSC1I64_01265
RTEGSU10_11605
AGYATC03_03845
AGMDCE93_00245
CARTPA27867_2020
CRYB7495_09995
MYLC3E77_14575
SALAESZ53_01560
AGGC1N71_12800
AGXAGREI_0926
BCVBcav_0157 Bcav_1677
IDOI598_2061(fgd1_2)
CETB8281_01045 B8281_02715
CCEUCBR64_09100 CBR64_10955
SKESked_00690
CFLCfla_3359
CFICelf_0191
CEZCBP52_12925
CELZE5225_00855 E5225_03090
CEJGC089_00780
CELHGXP71_17345
CIRALFM56_01045
OEKFFI11_002680
PSEIGCE65_14670
SERWFY030_06825
ORZFNH13_06330
HALTIM660_01200
MICGGJV80_12635
NCANoca_0856 Noca_1630
NDKI601_3217
NOYEXE57_05580
NANOG5V58_00785 G5V58_03785
NAROCFH99_04900
PSIMKR76_02295 KR76_07600
AEFGEV26_09440
KFLKfla_5429
KQIF1D05_08235
TCUTcur_0507 Tcur_2584
ACTWF7P10_01995 F7P10_02275 F7P10_05375 F7P10_37955
SROSros_4162
NOABKM31_20960
FREFranean1_4778
FRIFraEuI1c_2529 FraEuI1c_3450 FraEuI1c_3839
FALFRAAL3106 FRAAL4875
MMARMODMU_5441
KRAKrad_3725
AOIAORI_1327(hmd) AORI_2959
AJAAJAP_12490 AJAP_32920(hmd)
AMQAMETH_1112(hmd)
AMYCCU254_15235 CU254_17055
AABA4R43_03750 A4R43_05955 A4R43_15265
AMYYYIM_15240 YIM_19200(fgd7) YIM_38415
AORISD37_34555
AACDLWP59_32320
PDXPsed_0903 Psed_6467
PSEEFRP1_24510
PSEHXF36_06775
PSEQAD006_03695
PECQAD017_11515
PHHAFB00_19275
PAUTPdca_12860 Pdca_25040 Pdca_29260 Pdca_36040
PBROHOP40_21815 HOP40_22515 HOP40_24350 HOP40_29430 HOP40_33255
APRECNX65_14060
AMIAmir_2778
SESPBN6_46520
SSYIEKG83_30205
AHMTL08_05110
ACTIUA75_05060
ACADUA74_04945
AHGAHOG_04820
ACTAC1701_04395
PMADBAY61_05085 BAY61_09825 BAY61_22070 BAY61_24880
MAUMicau_1979
MILML5_2085
MICBMicB006_4996
MTUACSH63_15015
VMAVAB18032_19125
MCRAID554_06825 ID554_07060 ID554_30065 ID554_30185
ASEACPL_431
ACTNL083_0261
AFSAFR_28030
ACTSACWT_0316
PLABC6361_25880
PLATC6W10_12970
PFLAPflav_022530 Pflav_030270 Pflav_045200
PSUUPsuf_080170 Psuf_082450 Psuf_093780
VERHUT12_06135 HUT12_12195 HUT12_14575 HUT12_14800
ATLAthai_11400
PRYPrubr_38560(hmd_1) Prubr_57330 Prubr_59130
NHYJQS43_06565
EKEEK0264_07735
ABAIIMCC26256_111288 IMCC26256_111527
RMARGBA65_18010
BSOLFSW04_00070 FSW04_23130
CWOCwoe_5617
AYMYM304_13900
ERZER308_02385 ER308_09985 ER308_12970 ER308_13090
STISthe_2316 Sthe_2879
ATMANT_14450
ABATCFX1CAM_1029
PSUBPelsub_P1656 Pelsub_P1764
CAPCLDAP_07980 CLDAP_15430
PBFCFX0092_A2812
THUGKNN16_08060
PMETG4Y79_17220 G4Y79_17225
MJAMJ_1534
MFEMefer_1535
MVUMetvu_0160
MFSMFS40622_0752
MIFMetin_0159
MJHJH146_0139
MESGMLAUSG7_0920(mer)
MESAMLASG1_0449(mer)
MIGMetig_1293
MMPMMP0058(mer)
MMQMmarC5_1619
MMXMmarC6_0892
MMZMmarC7_1055
MMDGYY_00295
MMAKMMKA1_00600(mer)
MMAOMMOS7_00840(mer)
MMADMMJJ_10380
MAEMaeo_0749
MVNMevan_1073
MVOMvol_1374
MOKMetok_0200
METFCFE53_02485(mer)
MTHMTH_1752
MMGMTBMA_c03270(mer)
METCMTCT_1602
MWOMWSIV6_0292(mer)
METEtca_01698
METZMETMT2_1536
METKFVF72_02910(mer)
MTHMFZP57_01345(mer)
METJFZP68_06355(mer)
MSTMsp_1128(mer)
METBAW729_10140
MRUmru_0569(mer)
MSIMsm_0542
MEBAbm4_1417(mer)
MMILsm9_0419(mer)
MEYETL18_02180
MOLYLM1_0233
MAROMarbSA_08410
MELMetbo_0510
MEWMSWAN_1889
METHMBMB1_1520(mer)
MFCBRM9_0745(mer)
MFIDSM1535_0704(mer)
MCUBMCBB_1769(mer)
MSUBBK009_06580
METNBK008_02175
METTCIT01_07510(mer)
METOCIT02_10410(mer)
MEMEHYG87_08125(mer)
MFVMfer_0274
MKAMK0524(mer)
AFUAF_1066
AFGAFULGI_00011650
APOArcpr_1585
AVEArcve_0808 Arcve_1055
ASTAsulf_00466 Asulf_02156
FPLFerp_0743
GACGACE_0389
GAHGAH_01105
MBAMbar_A0254
MBYMSBRM_2698
MBWMSBRW_0741
MBARMSBR2_2654
MBAKMSBR3_0245 MSBR3_2771
MACMA_3733(mer)
MMAMM_0628
MMJMSMAS_0681
MMACMSMAC_0682
MVCMSVAZ_0679
MEKMSKOL_0657
MLSMSLAZ_2235
METMMSMTP_0746
MEFMSWH1_0878
MEQMSWHS_0937
MSJMSSAC_3543
MSZMSSIH_3084
MSWMSSIT_3142
MTHRMSTHT_2388
MTHEMSTHC_0892
MHORMSHOH_0799
MFZAOB57_003480(mer)
MBUMbur_2372(mer)
MMETMCMEM_0517
MELOJ7W08_00610(mer)
MMHMmah_1513
MHAZBHR79_06165
MEVMetev_2139
MZHMzhil_0168
MPYMpsy_2035
MZIHWN40_11975(mer)
MHZMetho_2247
MTPMthe_0205
MCJMCON_2394(mer)
MHIMhar_0856
MHUMhun_2257
MRTJKHC33_15695
MLAMlab_0076
MEMMemar_0557
MBGBN140_1674(mer)
MEMAMMAB1_2155(mer)
MCHKMchiMG62_06860
MPIMpet_2180
MBNMboo_0528
MFOMetfor_2659
MPLMpal_0327
MPDMCP_2681(mer)
MEZMtc_0135(mer)
RCIRCIX564(mer)
HDLHALDL1_03000
HHBHhub_1106(mer)
HNOLT974_14745
HLULT972_12970
HJEHacjB3_04915 HacjB3_13295
HSUHLASF_0416(mer)
HSFHLASA_0415(mer)
SALRFQU85_03600 FQU85_08510
HALREFA46_004740
HARAAArcS_2336
HAADMW046_10005
HALXM0R89_08695
HMArrnAC2437(mer1)
HHIHAH_2870(mer3)
HHNHISP_14590
HABSG26_06730
HTABVU17_13960
HALJG9465_05265
HSINKDQ40_15110
NPHNP_1056A(mer)
NMONmlp_1133(mer)
NHOHWV23_08180
NSALHWV07_10100
HUTHuta_1395
HTIHTIA_1288
HALAHrd1104_10095
HMUHmuk_0341
HALZE5139_04990
HALLLC1Hm_2870(mer)
HAZPGBQ70_04985
HALIBV210_10785
HSNDV733_10440
HDSHSR122_0385
HRRHZS55_02155
HPELHZS54_01595
HLTI7X12_11140
HARCHARCEL1_05640
HDOMUK72_11295
HWAHQ_3154A(mer)
HWCHqrw_3697(mer)
HVOHVO_1937(mer)
HMEHFX_2030(mer)
HGIABY42_09440
HALEG3A49_12675
HBOHbor_05330
HAQDU484_03660 DU484_04770
HAJDU500_04145 DU500_05210
HAERDU502_01690
HRAEI982_10810 EI982_11685
HLMDV707_14720
HALMFCF25_00710
HLAHlac_1680
HALPDOS48_08290
HALBEKH57_01810
HEZZEO776_10900
HALQHrr1229_013655
HSSJ7656_06825
HSAIHPS36_12235
HAZZKI388_07775
SRUBC2R22_17165
HAEhalTADL_0321 halTADL_1346
HALNB4589_005885
HALGHUG10_11070
HALUHUG12_00730 HUG12_05610
HMPK6T50_07940
HREK6T36_07775
HRMK6T25_08045
HACBHbl1158_14415
HAHHalar_1772
HTUHtur_3573
HDABB347_11940
HJTDVR14_10650
HALYHYG82_12020
HSALJMJ58_10490
HLOJ0X27_12155
HAKZJ0X25_05575 J0X25_19080 J0X25_19090
NMGNmag_1582(mer)
HXAHalxa_3407
NATNJ7G_2905
NPENatpe_0251
NVRFEJ81_01420
NPLFGF80_09575
NAYHYG81_09345
NGENatgr_2297
HRUHalru_2719
NOUNatoc_2482
SALIL593_00135
SAIMK0C01_04820
HLRHALLA_18435
HLCCHINAEXTREME06980
NAJB1756_04330
NAGAArcMg_0283
NANAArc1_0293
NBGDV706_06150
NASGCU68_07730
MELUMTLP_01530
MEAMMU439_00980
NIRNSED_04635
NKRNKOR_04660
NIDNPIRD3C_1360
NIWNisw_00540
NCLC5F47_04655
NOXC5F49_04565
NUEC5F50_06300
NVNNVIE_004930(mer)
NEVNTE_01814
NCVNCAV_1415
CSUCSUB_C0377 CSUB_C0386 CSUB_C0571 CSUB_C0806 CSUB_C1220 CSUB_C1712
TAHSU86_001930
CCAINAS2_0789
BARCAOA65_0782(mer_2)
 » show all
Reference
1  [PMID:2379499]
  Authors
Ma K, Thauer RK
  Title
Purification and properties of N5, N10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg).
  Journal
Eur J Biochem 191:187-93 (1990)
DOI:10.1111/j.1432-1033.1990.tb19109.x
  Sequence
Reference
2  [PMID:1911853]
  Authors
te Brommelstroet BW, Geerts WJ, Keltjens JT, van der Drift C, Vogels GD
  Title
Purification and properties of 5,10-methylenetetrahydromethanopterin dehydrogenase and 5,10-methylenetetrahydromethanopterin reductase, two coenzyme F420-dependent enzymes, from Methanosarcina barkeri.
  Journal
Biochim Biophys Acta 1079:293-302 (1991)
DOI:10.1016/0167-4838(91)90072-8
Reference
3  [PMID:1696553]
  Authors
Ma K, Thauer RK.
  Title
Single step purification of methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum by specific binding to blue sepharose CL-6B.
  Journal
FEBS Lett 268:59-62 (1990)
DOI:10.1016/0014-5793(90)80972-L
Reference
4  [PMID:2298726]
  Authors
te Brommelstroet BW, Hensgens CM, Keltjens JT, van der Drift C, Vogels GD
  Title
Purification and properties of 5,10-methylenetetrahydromethanopterin reductase, a coenzyme F420-dependent enzyme, from Methanobacterium thermoautotrophicum strain delta H.
  Journal
J Biol Chem 265:1852-7 (1990)
  Sequence
[mth:MTH_1752]
Reference
5  [PMID:2298699]
  Authors
te Brommelstroet BW, Hensgens CM, Geerts WJ, Keltjens JT, van der Drift C, Vogels GD.
  Title
Purification and properties of 5,10-methenyltetrahydromethanopterin cyclohydrolase from Methanosarcina barkeri.
  Journal
J Bacteriol 172:564-71 (1990)
DOI:10.1128/JB.172.2.564-571.1990
Other DBs
ExplorEnz - The Enzyme Database: 1.5.98.2
IUBMB Enzyme Nomenclature: 1.5.98.2
ExPASy - ENZYME nomenclature database: 1.5.98.2
BRENDA, the Enzyme Database: 1.5.98.2
LinkDB

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