KEGG   ENZYME: 1.8.98.4
Entry
EC 1.8.98.4                 Enzyme                                 

Name
coenzyme F420:CoB-CoM heterodisulfide,ferredoxin reductase;
hdrA2B2C2 (gene names)
Class
Oxidoreductases;
Acting on a sulfur group of donors;
With other, known, physiological acceptors
Sysname
CoB,CoM,ferredoxin:coenzyme F420 oxidoreductase
Reaction(IUBMB)
2 oxidized coenzyme F420 + 2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ = 2 reduced coenzyme F420 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB [RN:R11928]
Reaction(KEGG)
R11928
Substrate
oxidized coenzyme F420 [CPD:C00876];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
CoB [CPD:C04628];
CoM [CPD:C03576];
H+ [CPD:C00080]
Product
reduced coenzyme F420 [CPD:C01080];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
CoM-S-S-CoB [CPD:C04832]
Comment
The enzyme, characterized from the archaeon Methanosarcina acetivorans, catalyses the reduction of CoB-CoM heterodisulfide back to CoB and CoM. The enzyme consists of three components, HdrA, HdrB and HdrC, all of which contain [4Fe-4S] clusters. Electrons enter at HdrA, which also contains FAD, and are transferred via HdrC to the catalytic component, HdrB. During methanogenesis from acetate the enzyme catalyses the activity of EC 1.8.7.3, ferredoxin:CoB-CoM heterodisulfide reductase. However, it can also use electron bifurcation to direct electron pairs from reduced coenzyme F420 towards the reduction of both ferredoxin and CoB-CoM heterodisulfide. This activity is proposed to take place during Fe(III)-dependent anaerobic methane oxidation. cf. EC 1.8.98.5, H2:CoB-CoM heterodisulfide,ferredoxin reductase, EC 1.8.98.6, formate:CoB-CoM heterodisulfide,ferredoxin reductase, and EC 1.8.98.1, dihydromethanophenazine:CoB-CoM heterodisulfide reductase.
History
EC 1.8.98.4 created 2017
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K03388  heterodisulfide reductase subunit A2
K03389  heterodisulfide reductase subunit B2
K03390  heterodisulfide reductase subunit C2
Genes
TIG: THII_2280
THIS: HZT40_21385
TVI: Thivi_3111
TMB: Thimo_0247 Thimo_0248 Thimo_0253
TSY: THSYN_16425
HHC: M911_11215 M911_11220 M911_11230 M911_11235
EBS: ECTOBSL9_0282 ECTOBSL9_0283 ECTOBSL9_0285 ECTOBSL9_0286
TGR: Tgr7_2211 Tgr7_2212 Tgr7_2214 Tgr7_2215
TKM: TK90_0633 TK90_0634 TK90_0636 TK90_0637
TNI: TVNIR_3244(hdrB_[H]) TVNIR_3245(hdlC_[H]) TVNIR_3247(hdrA_[H]) TVNIR_3248(hdlB_[H])
SLIM: SCL_2520
TBN: TBH_C2777
ENM: EBS_2145(hdrB)
CBX: Cenrod_2147(hdrA)
SHD: SUTH_02769(hdrB)
TBD: Tbd_1645
SDR: SCD_n01881(hdrB)
GSU: GSU0090(hdrA) GSU0091(hdrB) GSU0092(hdrC)
GSK: KN400_0065(hdrA) KN400_0066(hdrB) KN400_0067(hdrC)
GME: Gmet_2084(bamE) Gmet_3424(hdrC) Gmet_3425(hdrB) Gmet_3426(hdrA)
GBM: Gbem_0030(hdrA) Gbem_0031(hdrB) Gbem_0032(hdrC) Gbem_1447(bamE)
GEO: Geob_0215(bamE-1) Geob_0231(bamE-2) Geob_3723(hdrA) Geob_3724(hdrB) Geob_3725(hdrC)
DVU: DVU2402(hdrA) DVU2403(hdrB) DVU2404(hdrC)
DMA: DMR_01900(hdrC) DMR_01910(hdrB) DMR_01920(hdrA) DMR_22120
DGG: DGI_1048(hdrC) DGI_1049(hdrB) DGI_1050(hdrA)
DTR: RSDT_0565(hdrC) RSDT_0566(hdrB) RSDT_0567(hdrA)
DPI: BN4_11858(hdrC) BN4_11859(hdrB) BN4_11860(hdrA)
DPS: DP1010
DML: Dmul_02770(hdrA1) Dmul_24500(bamE) Dmul_28860(hdrA2) Dmul_28870(hdrB) Dmul_28880(hdrC)
DAT: HRM2_06460 HRM2_11710(hdrA1) HRM2_11850(hdrA2) HRM2_14330(hdrA3) HRM2_14340(hdrA3') HRM2_22750(hdrA4) HRM2_22760(hdrD2) HRM2_22770(hdrD2')
DTO: TOL2_C02630 TOL2_C03750(bamE1) TOL2_C11590(hdlA2) TOL2_C11630(hdlA3?) TOL2_C17900(hdlA5) TOL2_C17910(hdlC) TOL2_C17920(hdlB) TOL2_C24180(hdlA6) TOL2_C25730(hdlA7) TOL2_C28760(hdlA8) TOL2_C28830(hdlA9) TOL2_C41400(bamE2) TOL2_C43540(bamE3)
ADE: Adeh_4164
ATX: GCD22_01577(hdrB1) GCD22_02661(hdrB1) GCD22_03145(hdrB) GCD22_03146(hdlC) GCD22_03148(hdrA) GCD22_03149(hdrB1) GCD22_03192(hdrB1) GCD22_03279(hdrA)
BTS: Btus_2496
PTH: PTH_1410(HdrA) PTH_1412(HdrB) PTH_1413(GlpC)
SAY: TPY_3529(hdrA)
STED: SPTER_13740 SPTER_13750(sdhE_1) SPTER_13760(mnmC)
AFO: Afer_0966
DFO: Dform_00920(hdrA) Dform_00941(hdrA) Dform_00942(hdrB) Dform_00943(hdrC)
TRO: trd_0153
ASH: AL1_26630
BACC: BRDCF_p1060 BRDCF_p823(hdrA) BRDCF_p824(hdrA2)
CTE: CT1245 CT1246(hdrA-2)
CTS: Ctha_0136
AAE: aq_391 aq_395(hdrA) aq_398(hdrC) aq_400(hdrB)
HTH: HTH_1878(hdrB1) HTH_1879(hdrC1) HTH_1881(hdrA) HTH_1882(hdrB2) HTH_1883(hdrC2)
CTHI: THC_1756
MMP: MMP0825(hdrA) MMP1053(hdrB2) MMP1054(hdrC2) MMP1154(hdrC1) MMP1155(hdrB1) MMP1697(hdrA)
MMAK: MMKA1_09100(hdrA) MMKA1_12180(hdrB) MMKA1_12190(hdrC) MMKA1_13320(hdrC) MMKA1_13330(hdrB) MMKA1_19310(hdrA)
MMAO: MMOS7_08880(hdrA) MMOS7_11480(hdrB) MMOS7_11490(hdrC) MMOS7_12620(hdrC) MMOS7_12630(hdrB) MMOS7_18120(hdrA)
MMG: MTBMA_c04490(hdrC) MTBMA_c04500(hdrB) MTBMA_c17680(hdrA)
MWO: MWSIV6_0409(hdrC) MWSIV6_0410(hdrB) MWSIV6_1762(hdrA)
METE: tca_01335(rsxB_2) tca_01814 tca_01815(lutA)
MST: Msp_1013(hdrB1) Msp_1014(hdrC1) Msp_1476(hdrA1)
MRU: mru_0117(hdrA) mru_0816(hdrC) mru_0817(hdrB1)
MEB: Abm4_1308(hdrB2) Abm4_1309(hdrC2) Abm4_1691(hdrA2)
MMIL: sm9_1716(hdrB3) sm9_1717(hdrC2) sm9_2251(hdrA2)
METH: MBMB1_1274(hdrB) MBMB1_1275 MBMB1_1958(hdrA)
MFC: BRM9_0490(hdrB1) BRM9_0491(hdrC1) BRM9_2298(hdrA2)
MFI: DSM1535_0471(hdrB) DSM1535_0472(hdrC) DSM1535_1232(hdrA)
MCUB: MCBB_1489(hdrB3) MCBB_1490(hdrC3) MCBB_2243(hdrA3)
MKA: MK0249(hdrA_1) MK0265(hdrA_2) MK0572(hdrB) MK0573(hdrC)
MEAR: Mpt1_c02960(hdrC1) Mpt1_c02970(hdrB1) Mpt1_c08830(hdrB2) Mpt1_c08840(hdrC2) Mpt1_c10580(hdrA)
MAC: MA_2868(hdrA) MA_4236(hdrC) MA_4237(hdrB)
MBU: Mbur_0552(hdrA) Mbur_1516(hdrC) Mbur_1517(hdrB)
MCJ: MCON_3279(hdrA) MCON_3282(hdrC) MCON_3283(hdrB)
MBG: BN140_0752(hdrA1) BN140_1726(hdrC) BN140_1727(hdrB) BN140_1728(hdrA3)
MEMA: MMAB1_0971(hdrA) MMAB1_2221(hdrC) MMAB1_2222(hdrB) MMAB1_2223(hdrA)
MPD: MCP_1576(hdrA-1) MCP_1577(hdrB-1) MCP_1578(hdrC-1) MCP_2768(hdrA-2) MCP_2769(hdrB-3) MCP_2770(hdrC-3)
MEZ: Mtc_2473(hdrA-2) Mtc_2474(hdrB-2) Mtc_2475(hdrC-2)
RCI: RCIX594(hdrC-2) RCIX595(hdrB-2) RCIX596(hdrA-2) RRC256(hdrC-3) RRC257(hdrB-3) RRC258(hdrA-3)
HDF: AArcSl_1855(hdrA)
SSO: SSO1127(hdrC-1) SSO1129(hdrB-1) SSO1131(hdrA) SSO1134(hdrC-2) SSO1135(hdrB-2)
BARC: AOA65_0030(hdrC_1) AOA65_1112(hdrA_1) AOA65_1400(hdrC_2) AOA65_1401(hdrB_2) AOA65_1402(hdrA_3) AOA65_1719(hdlA_2) AOA65_1887(hdrB_3) AOA65_1888(hdrC_3) AOA65_1993(hdrB_4) AOA65_1994(hdrC_4) AOA65_1995(hdrA_4)
BARB: AOA66_0155(hdrA_1) AOA66_1507(hdrB_1) AOA66_1508(hdrC_1) AOA66_1510(hdrA_2) AOA66_1512(hdlA_2) AOA66_1513(hdrA_3) AOA66_1526(hdrA_4) AOA66_1731(hdrB_2) AOA66_1732(hdrC_2)
LOKI: Lokiarch_00290(hdrA_1) Lokiarch_01240(hdrA_2) Lokiarch_01250(hdrC_1) Lokiarch_01260(hdrB_1) Lokiarch_03060(hdrC_2) Lokiarch_08680(hdrA_4) Lokiarch_09900(hdrA_5) Lokiarch_10040(hdlA_3) Lokiarch_17940(hdlA_5) Lokiarch_23580(hdrC_3) Lokiarch_23590(hdrB_2) Lokiarch_23600(hdrA_8) Lokiarch_23870(hdrC_4) Lokiarch_25880(hdrA_9) Lokiarch_28050(hdrA_10) Lokiarch_39150(hdrB_3) Lokiarch_39160(hdrC_5) Lokiarch_40200(hdrA_13) Lokiarch_41160(hdrA_15) Lokiarch_45500(hdrC_6) Lokiarch_45510(hdrB_4) Lokiarch_49330(hdrA_16)
PSYT: DSAG12_01136(hdrA_1) DSAG12_01137(hdrB_1) DSAG12_01138(hdrC_1) DSAG12_01145(hdrC_2) DSAG12_01146(hdrB_2) DSAG12_01147(hdrA_2) DSAG12_01161(hdrB_3) DSAG12_01162(hdrC_3) DSAG12_02253(hdrA_3) DSAG12_02805(hdrA_4) DSAG12_02806(hdrC_4) DSAG12_02807(hdrB_4) DSAG12_03218(hdrC_5) DSAG12_03220(hdrA_5) DSAG12_03223(hdrB_5) DSAG12_03721(hdrA_6) DSAG12_03723(hdlA_4) DSAG12_03724(hdrA_7) DSAG12_03725(hdrB_6) DSAG12_03726(hdrC_6) DSAG12_03952(hdrB_7) DSAG12_03953(hdrC_7)
 » show all
Reference
1  [PMID:28174314]
  Authors
Yan Z, Wang M, Ferry JG.
  Title
A Ferredoxin- and F420H2-Dependent, Electron-Bifurcating, Heterodisulfide Reductase with Homologs in the Domains Bacteria and Archaea.
  Journal
mBio 8:e02285-16 (2017)
DOI:10.1128/mBio.02285-16
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.8.98.4
IUBMB Enzyme Nomenclature: 1.8.98.4
ExPASy - ENZYME nomenclature database: 1.8.98.4
BRENDA, the Enzyme Database: 1.8.98.4
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