KEGG   ENZYME: 2.1.1.341Help
Entry
EC 2.1.1.341                Enzyme                                 

Name
vanillate/3-O-methylgallate O-demethylase;
ligM (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
vanillate:tetrahydrofolate O-methyltransferase
Reaction(IUBMB)
(1) vanillate + tetrahydrofolate = protocatechuate + 5-methyltetrahydrofolate [RN:R10136];
(2) 3-O-methylgallate + tetrahydrofolate = gallate + 5-methyltetrahydrofolate [RN:R09271]
Reaction(KEGG)
Substrate
vanillate [CPD:C06672];
tetrahydrofolate [CPD:C00101];
3-O-methylgallate [CPD:C05616]
Product
protocatechuate [CPD:C00230];
5-methyltetrahydrofolate [CPD:C00440];
gallate [CPD:C01424]
Comment
The enzyme, characterized from the bacterium Sphingomonas sp. SYK6, is involved in the degradation of lignin. The enzyme has similar activities with vanillate and 3-O-methylgallate.
History
EC 2.1.1.341 created 2017
Pathway
ec00627  Aminobenzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K15066  vanillate/3-O-methylgallate O-demethylase
Genes
CDN: BN940_04261
MAMO: A6B35_30230
MESO: BSQ44_07725 BSQ44_08460
ATU: Atu1420(gcvT)
AVI: Avi_5560(gcvT)
RLE: pRL90255
RLT: Rleg2_6553
RIR: BN877_p0379
RHT: NT26_2004(gcvT)
NGL: RG1141_CH21810 RG1141_CH37800
PTP: RCA23_c08390(gcvT1)
NAR: Saro_2861
SPHI: TS85_14190
SSY: SLG_12740(ligM)
AAY: WYH_01929(gcvT_1)
SBH: SBI_08592
BLIN: BLSMQ_1396
BSD: BLASA_2601(gcvT3)
MMAR: MODMU_2841(gcvT) MODMU_2842(gcvT) MODMU_2858
PDX: Psed_2464
PSEA: WY02_25075
STI: Sthe_2713
 » show all
Taxonomy
Reference
1  [PMID:9501423]
  Authors
Nishikawa S, Sonoki T, Kasahara T, Obi T, Kubota S, Kawai S, Morohoshi N, Katayama Y
  Title
Cloning and sequencing of the Sphingomonas (Pseudomonas) paucimobilis gene essential for the O demethylation of vanillate and syringate.
  Journal
Appl Environ Microbiol 64:836-42 (1998)
Reference
2  [PMID:15090517]
  Authors
Masai E, Sasaki M, Minakawa Y, Abe T, Sonoki T, Miyauchi K, Katayama Y, Fukuda M.
  Title
A novel tetrahydrofolate-dependent O-demethylase gene is essential for growth of  Sphingomonas paucimobilis SYK-6 with syringate.
  Journal
J Bacteriol 186:2757-65 (2004)
DOI:10.1128/JB.186.9.2757-2765.2004
Reference
3  [PMID:15743951]
  Authors
Abe T, Masai E, Miyauchi K, Katayama Y, Fukuda M.
  Title
A tetrahydrofolate-dependent O-demethylase, LigM, is crucial for catabolism of vanillate and syringate in Sphingomonas paucimobilis SYK-6.
  Journal
J Bacteriol 187:2030-7 (2005)
DOI:10.1128/JB.187.6.2030-2037.2005
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.341
IUBMB Enzyme Nomenclature: 2.1.1.341
ExPASy - ENZYME nomenclature database: 2.1.1.341
BRENDA, the Enzyme Database: 2.1.1.341
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