KEGG   ENZYME: 2.1.1.356
Entry
EC 2.1.1.356                Enzyme                                 

Name
[histone H3]-lysine27 N-trimethyltransferase;
KMT6A (gene name);
KMT6B (gene name);
EZH1 (gene name);
EZH2 (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:[histone H3]-L-lysine27 N6-trimethyltransferase
Reaction(IUBMB)
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine27 = 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27 (overall reaction);
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine27 = S-adenosyl-L-homocysteine + a [histone H3]-N6-methyl-L-lysine27;
(1b) S-adenosyl-L-methionine + a [histone H3]-N6-methyl-L-lysine27 = S-adenosyl-L-homocysteine + a [histone H3]-N6,N6-dimethyl-L-lysine27;
(1c) S-adenosyl-L-methionine + a [histone H3]-N6,N6-dimethyl-L-lysine27 = S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine27
Reaction(KEGG)
(other) R03875 R04866 R04867
Substrate
S-adenosyl-L-methionine [CPD:C00019];
[histone H3]-L-lysine27;
[histone H3]-N6-methyl-L-lysine27;
[histone H3]-N6,N6-dimethyl-L-lysine27
Product
S-adenosyl-L-homocysteine [CPD:C00021];
[histone H3]-N6,N6,N6-trimethyl-L-lysine27;
[histone H3]-N6-methyl-L-lysine27;
[histone H3]-N6,N6-dimethyl-L-lysine27
Comment
This entry describes enzymes that successively methylate the L-lysine27 residue of histone H3 (H3K27), ultimately generating a trimethylated form. These modifications influence the binding of chromatin-associated proteins. The methylation of lysine27 leads to transcriptional repression of the affected target genes. The enzyme associates with other proteins to form a complex that is essential for activity. The enzyme can also methylate some non-histone proteins. cf. EC 2.1.1.369, [histone H3]-lysine27 N-methyltransferase and EC 2.1.1.371, [histone H3]-lysine27 N-dimethyltransferase.
History
EC 2.1.1.356 created 1976 as EC 2.1.1.43, modified 1982, modified 1983, part transferred 2019 to EC 2.1.1.356, modified 2020
Pathway
ec00310  Lysine degradation
ec01100  Metabolic pathways
Orthology
K11430  [histone H3]-lysine27 N-trimethyltransferase EZH2
K17451  [histone H3]-lysine27 N-trimethyltransferase EZH1
Genes
HSA: 2145(EZH1) 2146(EZH2)
PTR: 454697(EZH1) 745437(EZH2)
PPS: 100967877(EZH1) 100971244(EZH2)
GGO: 101126515(EZH1) 101147968(EZH2)
PON: 100171872(EZH1) 100447447(EZH2)
NLE: 100580962(EZH2) 100604860(EZH1)
MCC: 709075(EZH2) 711173(EZH1)
MCF: 102130547(EZH1) 102146280(EZH2)
CSAB: 103227182(EZH2) 103243395(EZH1)
RRO: 104667012(EZH2) 104680126(EZH1)
RBB: 108522307(EZH2) 108526767(EZH1)
CJC: 100394758(EZH2) 100411881(EZH1)
SBQ: 101038203(EZH1) 101051611(EZH2)
MMU: 14055(Ezh1) 14056(Ezh2)
MCAL: 110295592(Ezh2) 110304611(Ezh1)
MPAH: 110315211(Ezh2) 110331872(Ezh1)
RNO: 303547(Ezh1) 312299(Ezh2)
MUN: 110547503(Ezh2) 110550881(Ezh1)
CGE: 100758055(Ezh2) 100760595(Ezh1)
NGI: 103731383(Ezh2) 103735912(Ezh1)
HGL: 101702710(Ezh2) 101723063(Ezh1)
CCAN: 109687337(Ezh1) 109695508(Ezh2)
OCU: 100345157(EZH2) 100351852(EZH1)
TUP: 102485788(EZH1) 102495627(EZH2)
CFA: 475511(EZH2) 480516(EZH1)
VVP: 112910738(EZH1) 112934818(EZH2)
AML: 100466177(EZH2) 100472619(EZH1)
UMR: 103668955(EZH2) 103672584(EZH1)
UAH: 113241452(EZH2) 113265406(EZH1)
ORO: 101369941(EZH2) 101379551(EZH1)
FCA: 101087309(EZH2) 101099602(EZH1)
PTG: 102956220(EZH2) 102961028(EZH1)
PPAD: 109258535(EZH1) 109269218(EZH2)
AJU: 106972615(EZH1) 106981407(EZH2)
BTA: 509106(EZH2) 533087(EZH1)
BOM: 102276653(EZH1) 102287858(EZH2)
BIU: 109557756(EZH2) 109573994(EZH1)
BBUB: 102405899(EZH2) 102407322(EZH1)
CHX: 102189343(EZH1) 102190101(EZH2)
OAS: 101104765(EZH1) 101120745(EZH2)
SSC: 100518394(EZH1) 100625497(EZH2)
CFR: 102515793(EZH2) 102516907(EZH1)
CDK: 105085196(EZH2) 105090473(EZH1)
LVE: 103069623(EZH2) 103073364(EZH1)
OOR: 101280796(EZH2) 101285746(EZH1)
DLE: 111166246(EZH1) 111177205(EZH2)
PCAD: 102994010(EZH2) 102994197(EZH1)
ECB: 100051290(EZH2) 100052411(EZH1)
EPZ: 103550351(EZH1) 103553703(EZH2)
EAI: 106826432(EZH1) 106842131(EZH2)
MYB: 102246299(EZH2) 102259730(EZH1)
MYD: 102755313(EZH1) 102768989(EZH2)
MNA: 107523768(EZH2) 107528927(EZH1)
HAI: 109395452(EZH2) 109395755(EZH1)
DRO: 112298572(EZH1) 112308348(EZH2)
PALE: 102879627(EZH1) 102886689(EZH2)
RAY: 107500533(EZH2) 107513838(EZH1)
MJV: 108386069(EZH1) 108386657(EZH2)
LAV: 100664312(EZH2) 100673691(EZH1)
MDO: 100010738(EZH1) 100013390(EZH2)
SHR: 100926678(EZH2) 100932535(EZH1)
PCW: 110198374(EZH1) 110201666(EZH2)
OAA: 100073485(EZH2) 100082851(EZH1)
GGA: 420023(EZH1) 420784(EZH2)
MGP: 100540374(EZH2) 100547448(EZH1)
CJO: 107309835(EZH2) 107325326(EZH1)
NMEL: 110388515(EZH1) 110390789(EZH2)
APLA: 101791765(EZH1) 101793453(EZH2)
ACYG: 106048025(EZH1) 106048735(EZH2)
TGU: 100226728(EZH2) 100227472(EZH1)
LSR: 110471820(EZH1) 110479186(EZH2)
SCAN: 103819367(EZH2) 103821570(EZH1)
GFR: 102035477(EZH1) 102038673(EZH2)
FAB: 101809240(EZH2) 101811414(EZH1)
PHI: 102099930(EZH2) 102103576(EZH1)
PMAJ: 107200653(EZH2) 107215126(EZH1)
CCAE: 111923642(EZH2) 111940096(EZH1)
CCW: 104688925(EZH2) 104697765(EZH1)
ETL: 114063607(EZH1) 114063865(EZH2)
FPG: 101911332(EZH2) 101915579(EZH1)
FCH: 102052374(EZH1) 102055584(EZH2)
CLV: 102093761(EZH2) 102097006(EZH1)
EGZ: 104125781(EZH2) 104134781(EZH1)
NNI: 104009878(EZH1) 104015388(EZH2)
ACUN: 113477549(EZH2) 113489070
PADL: 103916088(EZH2) 103919098(EZH1)
AAM: 106491114(EZH1) 106495267(EZH2)
ASN: 102372569(EZH2) 102383438(EZH1)
AMJ: 102562122(EZH2) 102566481(EZH1)
PSS: 102453330(EZH2) 102463087(EZH1)
CMY: 102932650(EZH2) 102934538(EZH1)
CPIC: 101934118(EZH1) 101937752(EZH2)
ACS: 100559669(ezh1) 100564989(ezh2)
PVT: 110080273(EZH1) 110088673(EZH2)
PBI: 103051581(EZH2) 103052924(EZH1)
PMUR: 107288847(EZH2) 107290401 107302416(EZH1)
TSR: 106547020(EZH2) 106554239(EZH1)
PMUA: 114581626(EZH1) 114607413(EZH2)
GJA: 107119624(EZH2) 107123351(EZH1)
XLA: 100381148(ezh2.S) 108701022(ezh1.L) 108702719(ezh1.S) 399174(ezh2.L)
XTR: 100493532(ezh1) 550047(ezh2)
NPR: 108794364(EZH2) 108796324(EZH1)
DRE: 664754(ezh1) 768133(ezh2)
IPU: 108256455(ezh2) 108279279(ezh1)
PHYP: 113528051(ezh1) 113541351(ezh2)
AMEX: 103027230(ezh2) 103044086(ezh1)
EEE: 113589064(ezh2) 113590056
TRU: 101068728(ezh2) 101070195(ezh1)
LCO: 104923910(ezh1) 104933871(ezh2)
NCC: 104940789(ezh2) 104944052
MZE: 101464777 101474304(ezh1) 112429845(ezh2)
ONL: 100695015(ezh1) 100702594(ezh2)
OLA: 100125823(ezh2) 101166385(ezh1)
XMA: 102218572(ezh2) 102228014(ezh1)
XCO: 114136427(ezh2) 114160264(ezh1)
PRET: 103457011(ezh2) 103468644(ezh1) 103469387
CVG: 107091776(ezh1) 107097879(ezh2)
NFU: 107378461(ezh1) 107383138(ezh2)
KMR: 108243802(ezh1) 108249555(ezh2)
ALIM: 106528219(ezh1) 106531656(ezh2)
AOCE: 111564547(ezh1) 111570645(ezh2)
CSEM: 103377188(ezh2) 103393149(ezh1)
POV: 109634330(ezh1)
LCF: 108884998(ezh2) 108900993(ezh1)
SDU: 111217595(ezh1) 111238320(ezh2)
SLAL: 111647273(ezh2) 111660565(ezh1)
HCQ: 109508596 109521815(ezh2)
BPEC: 110158320(ezh2) 110166859(ezh1)
MALB: 109951056(ezh2) 109964204(ezh1)
SALP: 111972867(ezh2) 111980227
ELS: 105013194(ezh1) 105022040(ezh2)
LCM: 102364190(EZH1)
CMK: 103187005(ezh2)
RTP: 109916301
BFO: 118425900
SPU: 585842
DME: Dmel_CG6502(E(z))
DER: 6546346
DSE: 6605167
DSI: Dsimw501_GD12857(Dsim_GD12857)
DAN: 6506463
DSR: 110185151
DPE: 6601386
DMN: 108151423
DWI: 6646360
DAZ: 108614083
DNV: 108652219
DVI: 6624012
MDE: 101887292
LCQ: 111677283
AAG: 5577466
AALB: 109401157
TCA: 659759
DPA: 109536169
NVL: 108560889
API: 100168406
DNX: 107161946
AGS: 114123060
RMD: 113551950
CEL: CELE_R06A4.7(mes-2)
BMY: Bm1_55060
NVE: 5501069
HMG: 100201207
ATH: AT2G23380(CLF) AT4G02020(SWN)
GMX: 100809840
GSJ: 114376685
VRA: 106777737
LJA: Lj2g3v1890940.1(Lj2g3v1890940.1) Lj2g3v1890940.2(Lj2g3v1890940.2)
FVE: 101314790
RCN: 112189431
PPER: 18770323
PMUM: 103339907
PAVI: 110765673
CSV: 101210052
CMO: 103497371
MCHA: 111017888
RCU: 8267339
SLY: 100134891(EZ1)
SPEN: 107001506
SOT: 102598283
CANN: 107840794
NSY: 104232085
NTO: 104099615
NAU: 109225973
DOSA: Os03t0307800-01(Os03g0307800) Os06t0275500-01(Os06g0275500)
SBI: 8083989
ZMA: 541955(mez3)
SITA: 101784446
 » show all
Reference
1  [PMID:12351676]
  Authors
Cao R, Wang L, Wang H, Xia L, Erdjument-Bromage H, Tempst P, Jones RS, Zhang Y
  Title
Role of histone H3 lysine 27 methylation in Polycomb-group silencing.
  Journal
Science 298:1039-43 (2002)
DOI:10.1126/science.1076997
  Sequence
[hsa:2146]
Reference
2  [PMID:12435631]
  Authors
Kuzmichev A, Nishioka K, Erdjument-Bromage H, Tempst P, Reinberg D
  Title
Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein.
  Journal
Genes Dev 16:2893-905 (2002)
DOI:10.1101/gad.1035902
  Sequence
[hsa:2146]
Reference
3  [PMID:15231737]
  Authors
Kirmizis A, Bartley SM, Kuzmichev A, Margueron R, Reinberg D, Green R, Farnham PJ
  Title
Silencing of human polycomb target genes is associated with methylation of histone H3 Lys 27.
  Journal
Genes Dev 18:1592-605 (2004)
DOI:10.1101/gad.1200204
  Sequence
[hsa:2146]
Reference
4  [PMID:17200670]
  Authors
Schlesinger Y, Straussman R, Keshet I, Farkash S, Hecht M, Zimmerman J, Eden E, Yakhini Z, Ben-Shushan E, Reubinoff BE, Bergman Y, Simon I, Cedar H
  Title
Polycomb-mediated methylation on Lys27 of histone H3 pre-marks genes for de novo  methylation in cancer.
  Journal
Nat Genet 39:232-6 (2007)
DOI:10.1038/ng1950
  Sequence
[hsa:2146]
Reference
5  [PMID:19026780]
  Authors
Shen X, Liu Y, Hsu YJ, Fujiwara Y, Kim J, Mao X, Yuan GC, Orkin SH
  Title
EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency.
  Journal
Mol Cell 32:491-502 (2008)
DOI:10.1016/j.molcel.2008.10.016
  Sequence
[mmu:14055 14056]
Reference
6  [PMID:21317239]
  Authors
Ezhkova E, Lien WH, Stokes N, Pasolli HA, Silva JM, Fuchs E
  Title
EZH1 and EZH2 cogovern histone H3K27 trimethylation and are essential for hair follicle homeostasis and wound repair.
  Journal
Genes Dev 25:485-98 (2011)
DOI:10.1101/gad.2019811
  Sequence
[mmu:14055 14056]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.356
IUBMB Enzyme Nomenclature: 2.1.1.356
ExPASy - ENZYME nomenclature database: 2.1.1.356
BRENDA, the Enzyme Database: 2.1.1.356
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