KEGG   ENZYME: 2.1.1.361
Entry
EC 2.1.1.361                Enzyme                                 

Name
[histone H4]-lysine20 N-methyltransferase;
KMT5A (gene name);
SET8 (gene name);
PR-SET7 (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:[histone H4]-L-lysine20 N6-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + a [histone H4]-L-lysine20 = S-adenosyl-L-homocysteine + a [histone H4]-N6-methyl-L-lysine20
Reaction(KEGG)
(other) R03875
Substrate
S-adenosyl-L-methionine [CPD:C00019];
[histone H4]-L-lysine20
Product
S-adenosyl-L-homocysteine [CPD:C00021];
[histone H4]-N6-methyl-L-lysine20
Comment
The enzyme catalyses the monomethylation of the L-lysine20 residue of histone H4 (H4K20). This event is usually followed by further methylation by EC 2.1.1.362, [histone H4]-N-methyl-L-lysine20 N-methyltransferase. This enzyme plays a pivotal role in DNA replication. Activity is high during the G2 and M phases, but declines significantly during G1 and S phases. Mutations in the enzyme have severe consequences, including DNA double-strand breaks, activation of DNA damage checkpoints, defective cell cycle progression, and reduced cell proliferation.
History
EC 2.1.1.361 created 1976 as EC 2.1.1.43, modified 1982, modified 1983, part transferred 2019 to EC 2.1.1.361
Pathway
ec00310  Lysine degradation
ec01100  Metabolic pathways
Orthology
K11428  [histone H4]-lysine20 N-methyltransferase SETD8
K20795  [histone H4]-lysine20 N-methyltransferase PRDM6
Genes
HSA: 387893(KMT5A) 93166(PRDM6)
PTR: 452343(KMT5A) 742318(PRDM6)
PPS: 100978154 100982491(PRDM6) 100986737(KMT5A)
GGO: 101143097(PRDM6) 101145513(KMT5A)
PON: 100451760(KMT5A) 100460132(PRDM6)
NLE: 100579681(PRDM6) 100580339(KMT5A)
MCC: 703499(PRDM6) 709345(KMT5A)
MCF: 102128021(KMT5A) 102142246(PRDM6)
CSAB: 103239328(KMT5A) 103244427(PRDM6)
RRO: 104660930(PRDM6) 104666320(KMT5A)
RBB: 108520391(PRDM6) 108540752(KMT5A)
CJC: 100411011(PRDM6) 100414548(KMT5A)
SBQ: 101040690(SETD8) 101047827(PRDM6)
MMU: 225518(Prdm6) 67956(Kmt5a)
MCAL: 110284774(Prdm6) 110295130(Kmt5a)
MPAH: 110312821(Kmt5a) 110333293(Prdm6)
RNO: 100361623(RGD1561318) 307305(Prdm6) 689820(Kmt5a)
CGE: 100773424(Prdm6) 100774411(Kmt5a)
NGI: 103738642(Prdm6) 103739033(Kmt5a)
HGL: 101720211(Prdm6) 101726359(Kmt5a)
CCAN: 109683525(Kmt5a) 109694403(Prdm6)
OCU: 100347868(KMT5A) 100354345(PRDM6) 100354805
TUP: 102471250(KMT5A) 102489496(PRDM6)
CFA: 100687856(PRDM6) 610615
VVP: 112910203(PRDM6)
AML: 100481447(PRDM6) 100482312(KMT5A)
UMR: 103661256(KMT5A) 103670579(PRDM6)
UAH: 113261649(PRDM6) 113266900(KMT5A)
ORO: 101370999(PRDM6) 101380066(SETD8)
PTG: 102956571(PRDM6) 102962714(KMT5A)
PPAD: 109277736(KMT5A) 109278870(PRDM6)
AJU: 106967577(KMT5A) 106975271(PRDM6)
BTA: 519857(PRDM6) 532622(KMT5A)
BOM: 102264795(KMT5A) 102279737(PRDM6)
BBUB: 102392447(KMT5A) 102401941(PRDM6)
CHX: 102176319(PRDM6) 102183718(KMT5A)
OAS: 101103235(PRDM6) 101122308(KMT5A)
SSC: 100524509(PRDM6) 100626555(KMT5A)
CFR: 102513845(PRDM6) 102521782(KMT5A)
CDK: 105102547(KMT5A) 105105065(PRDM6)
BACU: 103012927(KMT5A) 103016372(PRDM6)
LVE: 103079356(KMT5A) 103088820(PRDM6)
OOR: 101270549(SETD8) 101289146(PRDM6)
DLE: 111182365(PRDM6) 111186967(KMT5A)
PCAD: 102973557(PRDM6) 102996167(KMT5A)
ECB: 100060853(KMT5A) 100073092(PRDM6)
EPZ: 103549882(KMT5A) 103557946(PRDM6)
EAI: 106842331(KMT5A) 106842785(PRDM6)
MYD: 102763890(PRDM6) 102770785(KMT5A)
MNA: 107529655(KMT5A) 107540856(PRDM6)
HAI: 109379016(PRDM6) 109386114(KMT5A)
DRO: 112310724(KMT5A) 112314700(PRDM6)
PALE: 102889990(PRDM6) 102897510(KMT5A)
RAY: 107511140(PRDM6) 107515249(KMT5A)
MJV: 108400443(PRDM6) 108407461 108408425(KMT5A)
LAV: 100657495(PRDM6) 100673523(KMT5A)
MDO: 100014170(PRDM6) 100023739(KMT5A)
SHR: 100919577(PRDM6) 100933547(KMT5A)
PCW: 110196792(KMT5A) 110223799(PRDM6)
OAA: 100075898(KMT5A) 114808134(PRDM6)
GGA: 416825(KMT5A) 426800(PRDM6)
MGP: 100540244(KMT5A)
CJO: 107306730(PRDM6) 107321176(KMT5A)
NMEL: 110390181(PRDM6) 110406291(KMT5A)
APLA: 101791393(PRDM6) 101791981(KMT5A)
ACYG: 106031170(SETD8) 106046221(PRDM6)
TGU: 100225643(KMT5A) 100229699(PRDM6)
LSR: 110471779(KMT5A) 110479151(PRDM6)
GFR: 102037830(KMT5A) 102042417(PRDM6)
FAB: 101815311(PRDM6) 101821143(KMT5A)
PHI: 102107973(PRDM6) 102109183(KMT5A)
PMAJ: 107211636(KMT5A) 107216286(PRDM6)
CCAE: 111936365(KMT5A) 111941215(PRDM6)
CCW: 104688723(KMT5A) 104690502(PRDM6)
ETL: 114054956(KMT5A) 114055348(PRDM6)
FPG: 101914736(KMT5A) 101915352(PRDM6)
FCH: 102050773(PRDM6) 102053153(KMT5A)
CLV: 102089973(KMT5A) 102097159(PRDM6)
EGZ: 104124245(PRDM6) 104130151(SETD8)
NNI: 104012509(PRDM6) 104020902(SETD8)
ACUN: 113485973(KMT5A) 113489673(PRDM6)
PADL: 103914479(PRDM6) 103914547(SETD8)
AAM: 106484130 106497676(KMT5A)
ASN: 102380913(PRDM6) 102382842(KMT5A)
AMJ: 102569777(PRDM6) 102571182(KMT5A)
PSS: 102458837(PRDM6) 102463287(KMT5A)
CMY: 102929316(KMT5A) 102942167(PRDM6)
CPIC: 101932163(KMT5A) 101947533(PRDM6)
ACS: 100552008(prdm6) 100559474(kmt5a)
PVT: 110077521(PRDM6) 110083872(KMT5A)
PBI: 103053204(KMT5A) 103057070(PRDM6)
PMUR: 107285441(KMT5A) 107292729(PRDM6)
TSR: 106540545(SETD8) 106540743(PRDM6)
PMUA: 114586282(KMT5A) 114606447(PRDM6)
GJA: 107114655(PRDM6) 107117211(KMT5A)
XLA: 100158384(kmt5a.S) 108707198(prdm6.S) 108717331(prdm6.L) 398318(kmt5a.L)
XTR: 100494529(prdm6) 780276(kmt5a)
NPR: 108787307(KMT5A) 108799254(PRDM6)
DRE: 100004770(kmt5ab) 101886516(prdm6) 751629(kmt5aa)
PHYP: 113542401 113544710(kmt5a) 113547211(prdm6)
EEE: 113571045(prdm6) 113581614 113584033(kmt5a)
TRU: 101062904(kmt5a) 101063309(prdm6) 101068946
LCO: 104920299(kmt5a) 104935163(prdm6) 104940038
NCC: 104947636 104962306(setd8)
OLA: 101169793(kmt5a) 101172663 105354834(prdm6)
XMA: 102219947(kmt5a) 102220630(prdm6) 102233201
POV: 109626470(kmt5a) 109638204
LCF: 108874937(prdm6) 108878140 108897302(kmt5a)
SDU: 111217857(kmt5a) 111230938 111239241(prdm6)
MALB: 109953053 109957446(prdm6) 109961594(kmt5a)
ELS: 105014800(kmt5a) 105025387(prdm6) 105026110
SFM: 108934749(kmt5a) 108938054(prdm6)
PKI: 111856522(prdm6) 111860044(kmt5a)
LCM: 102355801(KMT5A) 102357740(PRDM6)
CMK: 103182382(prdm6) 103188010(kmt5a)
RTP: 109918332(kmt5a) 109930919(prdm6) 109938875
CIN: 100176062
APLC: 110987820
DME: Dmel_CG3307(PR-Set7)
DER: 6552524
DSE: 6606408
DSI: Dsimw501_GD18956(Dsim_GD18956)
DSR: 110176489
DPE: 6587245
DMN: 108155042
DWI: 6647761
DAZ: 108621090
DNV: 108659415
DHE: 111601499
DVI: 6631209
MDE: 101900242
LCQ: 111690642
AAG: 5570016
AME: 412027
BIM: 100745456
BTER: 100649334
CCAL: 108631655
OBB: 114877636
SOC: 105195427
MPHA: 105838593
AEC: 105146968
ACEP: 105619051
PBAR: 105426851
VEM: 105560777
HST: 105188422
DQU: 106743812
CFO: 105251700
LHU: 105673005
PGC: 109855440
OBO: 105281625
PCF: 106791652
MDL: 103578031
TCA: 657891
ATD: 109602979
NVL: 108557301
BMOR: 101738260
PMAC: 106709118
PRAP: 111004357
HAW: 110370812
API: 100161505
DNX: 107170040
AGS: 114119070
RMD: 113548056
BTAB: 109032364
CLEC: 106663755
ZNE: 110830787
FCD: 110853144
PVM: 113807185
DPTE: 113793328
CSCU: 111636826
PTEP: 107444515
CEL: CELE_T26A5.7(set-1)
CBR: CBG16281 CBG16282 CBG19678(Cbr-set-1)
BMY: Bm1_00260
PCAN: 112562936
OBI: 106878187
LAK: 106173811
SHX: MS3_05618
EGL: EGR_02931
DFA: DFA_00963
PCB: PCHAS_100250(PC000925.02.0)
TAN: TA21435
TPV: TP01_0281
BBO: BBOV_IV004680(23.m06513)
CPV: cgd4_370
 » show all
Reference
1  [PMID:12121615]
  Authors
Fang J, Feng Q, Ketel CS, Wang H, Cao R, Xia L, Erdjument-Bromage H, Tempst P, Simon JA, Zhang Y
  Title
Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase.
  Journal
Curr Biol 12:1086-99 (2002)
DOI:10.1016/s0960-9822(02)00924-7
  Sequence
[hsa:387893]
Reference
2  [PMID:12086618]
  Authors
Nishioka K, Rice JC, Sarma K, Erdjument-Bromage H, Werner J, Wang Y, Chuikov S, Valenzuela P, Tempst P, Steward R, Lis JT, Allis CD, Reinberg D
  Title
PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin.
  Journal
Mol Cell 9:1201-13 (2002)
DOI:10.1016/s1097-2765(02)00548-8
  Sequence
[hsa:387893]
Reference
3  [PMID:18166648]
  Authors
Jorgensen S, Elvers I, Trelle MB, Menzel T, Eskildsen M, Jensen ON, Helleday T, Helin K, Sorensen CS
  Title
The histone methyltransferase SET8 is required for S-phase progression.
  Journal
J Cell Biol 179:1337-45 (2007)
DOI:10.1083/jcb.200706150
  Sequence
[hsa:387893]
Reference
4  [PMID:19223465]
  Authors
Oda H, Okamoto I, Murphy N, Chu J, Price SM, Shen MM, Torres-Padilla ME, Heard E, Reinberg D
  Title
Monomethylation of histone H4-lysine 20 is involved in chromosome structure and stability and is essential for mouse development.
  Journal
Mol Cell Biol 29:2278-95 (2009)
DOI:10.1128/MCB.01768-08
  Sequence
[mmu:67956]
Reference
5  [PMID:23345616]
  Authors
Jorgensen S, Schotta G, Sorensen CS
  Title
Histone H4 lysine 20 methylation: key player in epigenetic regulation of genomic  integrity.
  Journal
Nucleic Acids Res 41:2797-806 (2013)
DOI:10.1093/nar/gkt012
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.361
IUBMB Enzyme Nomenclature: 2.1.1.361
ExPASy - ENZYME nomenclature database: 2.1.1.361
BRENDA, the Enzyme Database: 2.1.1.361
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