KEGG   ENZYME: 2.1.1.362
Entry
EC 2.1.1.362                Enzyme                                 

Name
[histone H4]-N-methyl-L-lysine20 N-methyltransferase;
KMT5B (gene name);
KMT5C (gene name);
SUV420H1 (gene name);
SUV420H2 (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:[histone H4]-N6-methyl-L-lysine20 N6-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + a [histone H4]-N6-methyl-L-lysine20 = S-adenosyl-L-homocysteine + a [histone H4]-N6,N6-dimethyl-L-lysine20
Reaction(KEGG)
(other) R04866
Substrate
S-adenosyl-L-methionine [CPD:C00019];
[histone H4]-N6-methyl-L-lysine20
Product
S-adenosyl-L-homocysteine [CPD:C00021];
[histone H4]-N6,N6-dimethyl-L-lysine20
Comment
This entry describes a group of enzymes that catalyse a single methylation of monomethylated lysine20 of histone H4 (H4K20m1, generated by EC 2.1.1.361, [histone H4]-lysine20 N-methyltransferase), forming the dimethylated form. This modification is broadly distributed across the genome and is likely important for general chromatin-mediated processes. The double-methylated form of lysine20 in histone H4 is the most abundant methylation state of this residue and is found on ~80% of all histone H4 molecules. Full activity of the enzyme requires that the lysine at position 9 of histone H3 is trimethylated.
History
EC 2.1.1.362 created 1976 as EC 2.1.1.43, modified 1982, modified 1983, part transferred 2019 to EC 2.1.1.362
Pathway
ec00310  Lysine degradation
ec01100  Metabolic pathways
Orthology
K11429  [histone H4]-N-methyl-L-lysine20 N-methyltransferase
Genes
HSA: 51111(KMT5B) 84787(KMT5C)
PTR: 451378(KMT5B) 735868(KMT5C)
PPS: 100984020(KMT5C) 100995372(KMT5B)
GGO: 101139439(KMT5C) 101151252(KMT5B)
PON: 100450271(KMT5B) 100450936(KMT5C)
NLE: 100583977(KMT5B) 100599527(KMT5C)
MCC: 699963(KMT5C) 710481(KMT5B)
MCF: 102117388(KMT5B) 102129054(KMT5C)
CSAB: 103215291(KMT5B) 103235303(KMT5C)
RRO: 104664716(KMT5B) 104674764(KMT5C)
RBB: 108518702(KMT5B) 108542541(KMT5C)
CJC: 100393036(KMT5C) 100400804(KMT5B)
SBQ: 101047990(SUV420H2) 101053072(SUV420H1)
MMU: 225888(Kmt5b) 232811(Kmt5c)
MCAL: 110285500(Kmt5b) 110298763(Kmt5c)
MPAH: 110334213(Kmt5b) 110336454(Kmt5c)
RNO: 308345(Kmt5c) 361688(Kmt5b)
MUN: 110547082(Kmt5b) 110561067(Kmt5c)
CGE: 100761878(Kmt5b) 100772952(Kmt5c)
NGI: 103744517(Kmt5b) 103744814(Kmt5c)
HGL: 101707567(Kmt5c) 101719039(Kmt5b)
CCAN: 109692079(Kmt5b) 109696272(Kmt5c)
OCU: 100340037(KMT5B)
TUP: 102474475 102497986(KMT5B) 102499452(KMT5C)
CFA: 483690(KMT5B) 484295(KMT5C)
VVP: 112909339(KMT5C) 112924166(KMT5B)
AML: 100468595(KMT5B) 100478438(KMT5C)
UMR: 103679527 103680304(KMT5C)
UAH: 113244004(KMT5B) 113247443(KMT5C)
ORO: 101374708(SUV420H2) 101375610(SUV420H1)
FCA: 101091322(KMT5C) 101094702(KMT5B)
PTG: 102953097(KMT5C) 102963329(KMT5B)
PPAD: 109246874(KMT5B) 109259888(KMT5C)
AJU: 106971172(KMT5B) 106980935(KMT5C)
BTA: 519777(KMT5C) 767828(KMT5B)
BOM: 102268312(KMT5B) 102288327(KMT5C)
BIU: 109572897(KMT5C)
BBUB: 102390793(KMT5C) 102405866(KMT5B)
CHX: 102185214(KMT5C) 102185720(KMT5B)
OAS: 101111001 101121289(KMT5C) 101121812(KMT5B)
SSC: 100521358(KMT5B) 110261054(KMT5C)
CFR: 102520054(KMT5B) 116666001(KMT5C)
CDK: 105098743(KMT5C) 105104405(KMT5B)
BACU: 102997321(KMT5C) 103007228(KMT5B)
LVE: 103090973(KMT5C) 103091082(KMT5B)
OOR: 101269348(SUV420H2) 101270232(SUV420H1)
DLE: 111181001(KMT5C) 111183648(KMT5B)
PCAD: 102982740(KMT5C) 102994432(KMT5B)
ECB: 100053438(KMT5B) 100055743(KMT5C)
EPZ: 103551272(KMT5B) 103562880(KMT5C)
EAI: 106840934(KMT5C) 106844276(KMT5B)
MYB: 102257692(KMT5B)
MYD: 102762755(KMT5B)
MNA: 107537430(KMT5B) 107541336(KMT5C)
HAI: 109373782(KMT5C) 109389302(KMT5B)
DRO: 112313746(KMT5C) 112317051(KMT5B)
PALE: 102887326(KMT5B) 102887846(KMT5C)
RAY: 107503474(KMT5B) 107515406(KMT5C)
MJV: 108388709(KMT5C) 108405830(KMT5B)
LAV: 100671600(KMT5B) 100671832(KMT5C)
MDO: 100010281(KMT5B)
SHR: 100917457(KMT5B)
PCW: 110195350(KMT5B) 110219937(KMT5C)
OAA: 100088070(KMT5B) 114814778(KMT5C)
GGA: 101748081(KMT5C) 425119(KMT5B)
MGP: 100547012(KMT5B) 116217397
CJO: 107307235(KMT5C) 107314523(KMT5B)
NMEL: 110391343(KMT5C) 110401096(KMT5B)
APLA: 101803754(KMT5B)
ACYG: 106038840(SUV420H1)
TGU: 100222904(KMT5B)
LSR: 110484490(KMT5B)
SCAN: 103824088(KMT5B)
GFR: 102041210(KMT5B)
FAB: 101805731(KMT5B)
PHI: 102102011(KMT5B) 102106962(KMT5C)
PMAJ: 107205911(KMT5B)
CCAE: 111930710(KMT5B)
CCW: 104692390(KMT5B)
ETL: 114064814(KMT5B)
FPG: 101921673(KMT5B)
FCH: 102046441(KMT5B)
CLV: 102091143(KMT5B)
EGZ: 104132445(SUV420H1)
NNI: 104017300(SUV420H1)
ACUN: 113481137(KMT5B)
PADL: 103919876(SUV420H1)
AAM: 106494851 106494860(KMT5B)
ASN: 102378990(KMT5C) 102379587(KMT5B)
AMJ: 102559156(KMT5C) 102568601(KMT5B)
PSS: 102456135(KMT5B)
CMY: 102946000(KMT5B)
CPIC: 101932218(KMT5C) 101950551(KMT5B)
ACS: 100553817(kmt5b) 100562840(suv420h2)
PVT: 110074171(KMT5B) 110078503(KMT5C)
PBI: 103053694(KMT5B) 103066601(KMT5C)
PMUR: 107282452(KMT5C) 107295878(KMT5B) 107296922
TSR: 106547726 106553569(SUV420H1)
PMUA: 114599913(KMT5B) 114608036(KMT5C)
GJA: 107118429(KMT5C) 107119069(KMT5B)
XLA: 108697809(kmt5c.S) 444204(kmt5b.S) 495826(kmt5b.L) 779432(kmt5c.L)
XTR: 100038141(kmt5b) 100145748(kmt5c)
NPR: 108784118(KMT5B) 108795144
DRE: 569041(kmt5c) 572849(kmt5b)
IPU: 108264420(suv420h1) 108271342 108280634
PHYP: 113534430(kmt5b) 113536364
AMEX: 103025343 103035158(kmt5b)
TRU: 101069597 101076428(kmt5b)
LCO: 104937792(kmt5b)
NCC: 104958752(suv420h1)
MZE: 101471827(kmt5b) 101477849
ONL: 100691614 100700783(kmt5b)
OLA: 101156070(kmt5b) 101165555
XMA: 102220708(kmt5b) 102236356
XCO: 114143669(kmt5b) 114159982
PRET: 103460097(kmt5b) 103468964
CVG: 107085059(kmt5b) 107096940
NFU: 107378497 107381531(kmt5b)
KMR: 108237783 108250521(kmt5b)
ALIM: 106532050(suv420h1)
AOCE: 111578621(kmt5b)
CSEM: 103379016(kmt5b)
POV: 109634192 109645819(kmt5b)
LCF: 108894245(kmt5b)
SDU: 111217719 111224033(kmt5b)
SLAL: 111656626(kmt5b) 111660544
HCQ: 109514373(kmt5b) 109527740
BPEC: 110168042(kmt5b)
MALB: 109951248(kmt5b) 109963137
ELS: 105023468 105029585(kmt5b)
SFM: 108934513(kmt5b) 108941728(kmt5c) 108942441
LCM: 102357247(KMT5C) 102357304(KMT5B)
CMK: 103174937(kmt5b) 103189530(kmt5c)
RTP: 109912567(kmt5b) 109932362
BFO: 118413910
CIN: 100186975
SPU: 583266
APLC: 110975028
SKO: 100372808
DME: Dmel_CG13363(Hmt4-20)
DER: 6555511
DSE: 6615887
DSI: Dsimw501_GD16466(Dsim_GD16466)
DSR: 110178198
DPE: 6600100
DMN: 108164669
DWI: 6644812
DAZ: 108618025
DNV: 108655611
DHE: 111596240
MDE: 101897840
LCQ: 111679605
AME: 411906
BIM: 100742553
BTER: 100644080
CCAL: 108624513
OBB: 114881313
SOC: 105195127
MPHA: 105830481
AEC: 105144221
ACEP: 105627042
PBAR: 105427527
VEM: 105568890
HST: 105183369
DQU: 106749629
CFO: 105255356
LHU: 105672756
PGC: 109853314
OBO: 105280691
PCF: 106793217
CSOL: 105362524
TCA: 663776
DPA: 109533054
ATD: 109597721
NVL: 108569827
BMOR: 101735557
BMAN: 114248506
PMAC: 106708893
PRAP: 110992850
HAW: 110372275
TNL: 113508300
PXY: 105397967
RMD: 113549136
BTAB: 109030108
CLEC: 106669134
ZNE: 110835364
TUT: 107371540
DPTE: 113794752
CSCU: 111636078
PTEP: 107455782
CEL: CELE_C32D5.5(set-4)
CBR: CBG02629(Cbr-set-4) CBG14502
BMY: Bm1_06250
PCAN: 112560425
CRG: 105319029
MYI: 110457084
OBI: 106875752
SHX: MS3_08308
EGL: EGR_03818
NVE: 5514661
EPA: 110245633
ADF: 107353716
AMIL: 114956880
PDAM: 113667740
SPIS: 111325445
DGT: 114533764
HMG: 100207998
QSU: 112005838
NCR: NCU08551
NTE: NEUTE1DRAFT82859(NEUTE1DRAFT_82859)
MGR: MGG_07393
SSCK: SPSK_07036
NHE: NECHADRAFT_12117(SDG2118)
MAW: MAC_03332
MAJ: MAA_07113
CMT: CCM_08113
MBE: MBM_09131
ANI: AN6147.2
ANG: ANI_1_476104(An12g03880)
ABE: ARB_04917
TVE: TRV_05893
PTE: PTT_18535
CNE: CNC03710
CNB: CNBC3500
TASA: A1Q1_07214
ABP: AGABI1DRAFT119579(AGABI1DRAFT_119579)
ABV: AGABI2DRAFT185465(AGABI2DRAFT_185465)
MGL: MGL_1066
MRT: MRET_3089
 » show all
Reference
1  [PMID:15145825]
  Authors
Schotta G, Lachner M, Sarma K, Ebert A, Sengupta R, Reuter G, Reinberg D, Jenuwein T
  Title
A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin.
  Journal
Genes Dev 18:1251-62 (2004)
DOI:10.1101/gad.300704
  Sequence
Reference
2  [PMID:23345616]
  Authors
Jorgensen S, Schotta G, Sorensen CS
  Title
Histone H4 lysine 20 methylation: key player in epigenetic regulation of genomic  integrity.
  Journal
Nucleic Acids Res 41:2797-806 (2013)
DOI:10.1093/nar/gkt012
Reference
3  [PMID:24396869]
  Authors
Wu H, Siarheyeva A, Zeng H, Lam R, Dong A, Wu XH, Li Y, Schapira M, Vedadi M, Min J
  Title
Crystal structures of the human histone H4K20 methyltransferases SUV420H1 and SUV420H2.
  Journal
FEBS Lett 587:3859-68 (2013)
DOI:10.1016/j.febslet.2013.10.020
  Sequence
[hsa:51111 84787]
Reference
4  [PMID:24049080]
  Authors
Southall SM, Cronin NB, Wilson JR
  Title
A novel route to product specificity in the Suv4-20 family of histone H4K20 methyltransferases.
  Journal
Nucleic Acids Res 42:661-71 (2014)
DOI:10.1093/nar/gkt776
  Sequence
Reference
5  [PMID:27105552]
  Authors
Weirich S, Kudithipudi S, Jeltsch A
  Title
Specificity of the SUV4-20H1 and SUV4-20H2 protein lysine methyltransferases and  methylation of novel substrates.
  Journal
J Mol Biol 428:2344-2358 (2016)
DOI:10.1016/j.jmb.2016.04.015
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.362
IUBMB Enzyme Nomenclature: 2.1.1.362
ExPASy - ENZYME nomenclature database: 2.1.1.362
BRENDA, the Enzyme Database: 2.1.1.362
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