Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy

acyl-CoA:malonyl-CoA C-acyltransferase (2-methylbutanoate-forming)

2 malonyl-CoA + [2-methylbutanoate polyketide synthase] + 2 NADPH + 3 H+ + S-adenosyl-L-methionine = (S)-2-methylbutanoyl-[2-methylbutanoate polyketide synthase] + 2 CoA + 2 CO2 + 2 NADP+ + S-adenosyl-L-homocysteine + H2O [RN:R11445]

R11445
Reaction

malonyl-CoA [CPD:C00083];
[2-methylbutanoate polyketide synthase];
NADPH [CPD:C00005];
H+ [CPD:C00080];
S-adenosyl-L-methionine [CPD:C00019]

This polyketide synthase enzyme forms the (S)-2-methylbutanoate side chain during lovastatin biosynthesis by the filamentous fungus Aspergillus terreus. The overall reaction comprises a single condensation reaction followed by alpha-methylation, beta-ketoreduction, dehydration, and alpha,beta-enoyl reduction.

EC 2.3.1.244 created 2015, modified 2016

1  [PMID:10334994]

Kennedy J, Auclair K, Kendrew SG, Park C, Vederas JC, Hutchinson CR

Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis.

Science 284:1368-72 (1999)
DOI:10.1126/science.284.5418.1368

[ag:AAD34559]

2  [PMID:21069965]

Meehan MJ, Xie X, Zhao X, Xu W, Tang Y, Dorrestein PC

FT-ICR-MS characterization of intermediates in the biosynthesis of the alpha-methylbutyrate side chain of lovastatin by the 277 kDa polyketide synthase LovF.

Biochemistry 50:287-99 (2011)
DOI:10.1021/bi1014776

[ag:AAD34559]