KEGG   ENZYME: 2.3.1.246Help
Entry
EC 2.3.1.246                Enzyme                                 

Name
3,5-dihydroxyphenylacetyl-CoA synthase;
DpgA
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
malonyl-CoA:malonyl-CoA malonyltransferase (3,5-dihydroxyphenylacetyl-CoA-forming)
Reaction(IUBMB)
4 malonyl-CoA = (3,5-dihydroxyphenylacetyl)-CoA + 3 CoA + 4 CO2 + H2O [RN:R06625]
Reaction(KEGG)
Substrate
malonyl-CoA [CPD:C00083]
Product
3,5-dihydroxyphenylacetyl-CoA [CPD:C12324];
CoA [CPD:C00010];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Amycolatopsis mediterranei, is involved in biosynthesis of the nonproteinogenic amino acid (S)-3,5-dihydroxyphenylglycine, a component of the vancomycin-type antibiotic balhimycin.
History
EC 2.3.1.246 created 2015
Pathway
ec01055  Biosynthesis of vancomycin group antibiotics
Orthology
K16424  3,5-dihydroxyphenylacetyl-CoA synthase
Genes
SMAF: D781_2331
SERM: CLM71_12100
XAL: XALC_1059
XTN: FD63_09790
PAND: DRB87_09790
MNR: ACZ75_13535
BSTO: C0V70_02630
NBR: O3I_032820
NTP: CRH09_30870 CRH09_31745
SVE: SVEN_0493
SLE: sle_03950(sle_03950) sle_58620(sle_58620)
SMAL: SMALA_7737
KFL: Kfla_4132
NDA: Ndas_1740
AOI: AORI_1502(bcsA) AORI_5330(bcsA)
MOX: DAMO_0702
 » show all
Taxonomy
Reference
1  [PMID:11495926]
  Authors
Pfeifer V, Nicholson GJ, Ries J, Recktenwald J, Schefer AB, Shawky RM, Schroder J, Wohlleben W, Pelzer S
  Title
A polyketide synthase in glycopeptide biosynthesis: the biosynthesis of the non-proteinogenic amino acid (S)-3,5-dihydroxyphenylglycine.
  Journal
J Biol Chem 276:38370-7 (2001)
DOI:10.1074/jbc.M106580200
  Sequence
Reference
2  [PMID:11752437]
  Authors
Chen H, Tseng CC, Hubbard BK, Walsh CT
  Title
Glycopeptide antibiotic biosynthesis: enzymatic assembly of the dedicated amino acid monomer (S)-3,5-dihydroxyphenylglycine.
  Journal
Proc Natl Acad Sci U S A 98:14901-6 (2001)
DOI:10.1073/pnas.221582098
Reference
3  [PMID:14744141]
  Authors
Tseng CC, McLoughlin SM, Kelleher NL, Walsh CT
  Title
Role of the active site cysteine of DpgA, a bacterial type III polyketide synthase.
  Journal
Biochemistry 43:970-80 (2004)
DOI:10.1021/bi035714b
Reference
4  [PMID:22492619]
  Authors
Wu HC, Li YS, Liu YC, Lyu SY, Wu CJ, Li TL
  Title
Chain elongation and cyclization in type III PKS DpgA.
  Journal
Chembiochem 13:862-71 (2012)
DOI:10.1002/cbic.201200051
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.246
IUBMB Enzyme Nomenclature: 2.3.1.246
ExPASy - ENZYME nomenclature database: 2.3.1.246
BRENDA, the Enzyme Database: 2.3.1.246
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