KEGG   ENZYME: 2.3.1.252Help
Entry
EC 2.3.1.252                Enzyme                                 

Name
mycolipanoate synthase;
msl3 (gene name);
Pks3/4;
mycolipanoic acid synthase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
long-chain acyl-CoA:methylmalonyl-CoA C-acyltransferase (mycolipanoate-forming)
Reaction(IUBMB)
a long-chain acyl-CoA + 3 (R)-methylmalonyl-CoA + 6 NADPH + 6 H+ + holo-[mycolipanoate synthase] = mycolipanoyl-[mycolipanoate synthase] + 4 CoA + 3 CO2 + 6 NADP+ + 3 H2O
Reaction(KEGG)
(other) R05189 R11451
Show
Substrate
long-chain acyl-CoA [CPD:C02843];
(R)-methylmalonyl-CoA [CPD:C01213];
NADPH [CPD:C00005];
H+ [CPD:C00080];
holo-[mycolipanoate synthase]
Product
mycolipanoyl-[mycolipanoate synthase];
CoA [CPD:C00010];
CO2 [CPD:C00011];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
This mycobacterial enzyme accepts long-chain fatty acyl groups from their CoA esters and extends them by incorporation of three methylmalonyl (but not malonyl) residues, forming trimethyl-branched fatty-acids such as (2S,4S,6S)-2,4,6-trimethyltetracosanoate (C27-mycolipanoate). Since the enzyme lacks a thioesterase domain, the product remains bound to the enzyme and requires additional enzyme(s) for removal.
History
EC 2.3.1.252 created 2016
Orthology
K12432  mycolipanoate synthase
Genes
MTU: Rv1180(pks3) Rv1181(pks4)
MTV: RVBD_1181
MTC: MT1218(mas-1)
MRA: MRA_1191(pks3)
MTF: TBFG_11205
MTB: TBMG_02801
MTK: TBSG_02815
MTZ: TBXG_002781
MTE: CCDC5079_1092
MTUR: CFBS_1259(pks3)
MTD: UDA_1180(pks3) UDA_1181(pks4)
MTN: ERDMAN_1323(pks4)
MTUB: MT7199_1210(pks3_4)
MTUC: J113_08285
MTUE: J114_06380
MTUL: TBHG_01164
MTUT: HKBT1_1257(pks3)
MTUU: HKBT2_1263(pks3)
MTQ: HKBS1_1261(pks3)
MBO: BQ2027_MB1213(pks3)
MBB: BCG_1243(pks3)
MBT: JTY_1216(pks3)
MBM: BCGMEX_1215(pks3)
MBX: BCGT_1012
MAF: MAF_11990(pks3) MAF_12000(pks4)
MMIC: RN08_1325
MCE: MCAN_11911(pks3) MCAN_11921(pks4)
MCQ: BN44_11320(pks)
MCV: BN43_30248(pks)
MCX: BN42_21046(pks)
MCZ: BN45_30243(pks)
MLE: ML1229(pks3)
MLB: MLBr01229(pks3)
MAV: MAV_1321
MIT: OCO_12290
MIA: OCU_12250
MID: MIP_01963
MYO: OEM_12440
MIR: OCQ_12310
MLP: MLM_1249
MMM: W7S_06000
MHAD: B586_07465
MSHG: MSG_03827(pks3)
 » show all
Taxonomy
Reference
1  [PMID:11278910]
  Authors
Sirakova TD, Thirumala AK, Dubey VS, Sprecher H, Kolattukudy PE
  Title
The Mycobacterium tuberculosis pks2 gene encodes the synthase for the hepta- and  octamethyl-branched fatty acids required for sulfolipid synthesis.
  Journal
J Biol Chem 276:16833-9 (2001)
DOI:10.1074/jbc.M011468200
Reference
2  [PMID:12207710]
  Authors
Dubey VS, Sirakova TD, Kolattukudy PE
  Title
Disruption of msl3 abolishes the synthesis of mycolipanoic and mycolipenic acids  required for polyacyltrehalose synthesis in Mycobacterium tuberculosis H37Rv and  causes cell aggregation.
  Journal
Mol Microbiol 45:1451-9 (2002)
DOI:10.1046/j.1365-2958.2002.03119.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.252
IUBMB Enzyme Nomenclature: 2.3.1.252
ExPASy - ENZYME nomenclature database: 2.3.1.252
BRENDA, the Enzyme Database: 2.3.1.252
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