KEGG   ENZYME: 2.3.1.271
Entry
EC 2.3.1.271                Enzyme                                 

Name
L-glutamate-5-semialdehyde N-acetyltransferase;
MPR1 (gene name);
MPR2 (gene name)
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
acetyl-CoA:L-glutamate-5-semialdehyde N-acetyltransferase
Reaction(IUBMB)
acetyl-CoA + L-glutamate-5-semialdehyde = CoA + N-acetyl-L-glutamate 5-semialdehyde [RN:R12085]
Reaction(KEGG)
R12085
Substrate
acetyl-CoA [CPD:C00024];
L-glutamate-5-semialdehyde
Product
CoA [CPD:C00010];
N-acetyl-L-glutamate 5-semialdehyde [CPD:C01250]
Comment
The enzyme, characterized from the yeast Saccharomyces cerevisiae Sigma1278b, N-acetylates L-glutamate-5-semialdehyde, an L-proline biosynthesis/utilization intermediate, into N-acetyl-L-glutamate 5-semialdehyde, an intermediate of L-arginine biosynthesis, under oxidative stress conditions. Its activity results in conversion of L-proline to L-arginine, and reduction in the concentration of L-glutamate 5-semialdehyde and its equilibrium partner, (S)-1-pyrroline-5-carboxylate, which has been linked to production of reactive oxygen species stress. The enzyme also acts on (S)-1-acetylazetidine-2-carboxylate, a toxic L-proline analog produced by some plants, resulting in its detoxification and conferring resistance on the yeast.
History
EC 2.3.1.271 created 2018
Pathway
ec00330  Arginine and proline metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K22842  L-glutamate-5-semialdehyde N-acetyltransferase
Genes
KLA: KLLA0_F11440g
KMX: KLMA_20195(ppr1)
LTH: KLTH0D10934g
ZRO: ZYRO0F07062g
NCS: NCAS_0B01570(NCAS0B01570)
NDI: NDAI_0E01220(NDAI0E01220)
TPF: TPHA_0D01180(TPHA0D01180)
TDL: TDEL_0A06090(TDEL0A06090)
KAF: KAFR_0A03800(KAFR0A03800)
YLI: YALI0E09999g
Reference
1  [PMID:11555637]
  Authors
Shichiri M, Hoshikawa C, Nakamori S, Takagi H
  Title
A novel acetyltransferase found in Saccharomyces cerevisiae Sigma1278b that detoxifies a proline analogue, azetidine-2-carboxylic acid.
  Journal
J Biol Chem 276:41998-2002 (2001)
DOI:10.1074/jbc.C100487200
  Sequence
Reference
2  [PMID:15308773]
  Authors
Nomura M, Takagi H
  Title
Role of the yeast acetyltransferase Mpr1 in oxidative stress: regulation of oxygen reactive species caused by a toxic proline catabolism intermediate.
  Journal
Proc Natl Acad Sci U S A 101:12616-21 (2004)
DOI:10.1073/pnas.0403349101
  Sequence
Reference
3  [PMID:20550582]
  Authors
Nishimura A, Kotani T, Sasano Y, Takagi H
  Title
An antioxidative mechanism mediated by the yeast N-acetyltransferase Mpr1: oxidative stress-induced arginine synthesis and its physiological role.
  Journal
FEMS Yeast Res 10:687-98 (2010)
DOI:10.1111/j.1567-1364.2010.00650.x
  Sequence
Reference
4  [PMID:22698729]
  Authors
Nishimura A, Nasuno R, Takagi H
  Title
The proline metabolism intermediate Delta1-pyrroline-5-carboxylate directly inhibits the mitochondrial respiration in budding yeast.
  Journal
FEBS Lett 586:2411-6 (2012)
DOI:10.1016/j.febslet.2012.05.056
Reference
5  [PMID:23818613]
  Authors
Nasuno R, Hirano Y, Itoh T, Hakoshima T, Hibi T, Takagi H
  Title
Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism.
  Journal
Proc Natl Acad Sci U S A 110:11821-6 (2013)
DOI:10.1073/pnas.1300558110
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.271
IUBMB Enzyme Nomenclature: 2.3.1.271
ExPASy - ENZYME nomenclature database: 2.3.1.271
BRENDA, the Enzyme Database: 2.3.1.271
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