KEGG   ENZYME: 2.3.1.293
Entry
EC 2.3.1.293                Enzyme                                 
Name
meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase I;
kasA (gene name);
beta-ketoacyl-acyl carrier protein synthase KasA
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
ultra-long-chain mono-unsaturated fattyl acyl-[acyl-carrier protein]:malonyl-[acyl-carrier protein] C-acyltransferase (decarboxylating)
Reaction(IUBMB)
an ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = an ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + a holo-[acyl-carrier protein]
Substrate
ultra-long-chain mono-unsaturated acyl-[acyl-carrier protein];
malonyl-[acyl-carrier protein] [CPD:C01209]
Product
ultra-long-chain mono-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein];
CO2 [CPD:C00011];
holo-[acyl-carrier protein] [CPD:C00229]
Comment
The enzyme is part of the fatty acid synthase (FAS) II system of mycobacteria, which extends modified products of the FAS I system, eventually forming meromycolic acids that are incorporated into mycolic acids. Meromycolic acids consist of a long chain, typically 50-60 carbons, which is functionalized by different groups.Two 3-oxoacyl-(acyl carrier protein) synthases function within the FAS II system, encoded by the kasA and kasB genes. The two enzymes share some sequence identity but function independently on separate sets of substrates. KasA differs from KasB [EC 2.3.1.294, meromycolic acid 3-oxoacyl-(acyl carrier protein) synthase II], by preferring shorter (C-22 to C-36) and more saturated (only one double bond) substrates.
History
EC 2.3.1.293 created 2019
Orthology
K11609  beta-ketoacyl ACP synthase
Genes
MTURv2245(kasA) Rv2246(kasB)
MTVRVBD_2245 RVBD_2246
MTCMT2305(fabF-1) MT2306(fabF-2)
MRAMRA_2265(kasA) MRA_2266(kasB)
MTFTBFG_12274 TBFG_12275
MTBTBMG_01734 TBMG_01735
MTKTBSG_01744 TBSG_01745
MTZTBXG_001716 TBXG_001717
MTGMRGA327_13860
MTIMRGA423_13980 MRGA423_13985
MTECCDC5079_2082 CCDC5079_2083
MTURCFBS_2378 CFBS_2379(kasB)
MTLCCDC5180_2054 CCDC5180_2055
MTOMTCTRI2_2281(kasA) MTCTRI2_2282(kasB)
MTDUDA_2245(kasA) UDA_2246(kasB)
MTNERDMAN_2470(kasA) ERDMAN_2471(kasB)
MTJJ112_12055 J112_12060
MTUBMT7199_2277 MT7199_2278
MTUEJ114_12035 J114_12040
MTXM943_11630 M943_11635
MTUHI917_15810
MTULTBHG_02197 TBHG_02198
MTUTHKBT1_2369 HKBT1_2370(kasB)
MTUUHKBT2_2371 HKBT2_2372(kasB)
MTQHKBS1_2375 HKBS1_2376(kasB)
MBOBQ2027_MB2269(kasA) BQ2027_MB2270(kasB)
MBBBCG_2262(kasA) BCG_2263(kasB)
MBTJTY_2256(kasA) JTY_2257(kasB)
MBMBCGMEX_2250(kasA) BCGMEX_2251(kasB)
MBKK60_023300 K60_023310
MBXBCGT_2068 BCGT_2069
MAFMAF_22560(kasA) MAF_22570(kasB)
MMICRN08_2493 RN08_2494
MCEMCAN_22671(kasA) MCAN_22681(kasB)
MCQBN44_50188(kasA) BN44_50189(kasB)
MCVBN43_31488(kasA) BN43_31489(kasB)
MCXBN42_40169(kasA) BN42_40170(kasB)
MCZBN45_50577(kasA) BN45_50578(kasB)
MLEML1655(kasA) ML1656(kasB)
MLBMLBr01655(kasA) MLBr01656(kasB)
MPAMAP_1998(kasA) MAP_1999(kasB) MAP_3485(kasB)
MAOMAP4_0292 MAP4_1826 MAP4_1827
MAVIRC58_09125 RC58_09130
MAVURE97_09130 RE97_09135
MAVMAV_2191 MAV_2192 MAV_4343
MITOCO_21780 OCO_21790 OCO_42090
MIAOCU_21960 OCU_21970 OCU_42010
MIDMIP_03076 MIP_03078 MIP_06344
MYOOEM_19670 OEM_19680 OEM_42440
MCHIAN480_11270 AN480_11275 AN480_22070
MIROCQ_20880 OCQ_20900 OCQ_43360
MMALCKJ54_09720 CKJ54_09725 CKJ54_20115
MLPMLM_1920 MLM_1921 MLM_3503
MMANMMAN_18020(kasA) MMAN_18030(kasB_1) MMAN_30190(kasB_2)
MSAMycsm_00734 Mycsm_03980 Mycsm_03981
MULMUL_1303(kasB) MUL_1304(kasA)
MMCMmcs_0783 Mmcs_3366 Mmcs_3367
MKMMkms_0798 Mkms_3428 Mkms_3429
MJLMjls_0779 Mjls_3377 Mjls_3378
MMIMMAR_3338(kasA) MMAR_3339(kasB)
MMAEMMARE11_32390(kasA) MMARE11_32400(kasB)
MMMW7S_10240 W7S_10245 W7S_21055
MLIMULP_03599(kasA) MULP_03600(kasB)
MKNMKAN_03345 MKAN_03350
MYVG155_18575 G155_18580 G155_24920
MYEAB431_04975 AB431_19260 AB431_19265
MGOAFA91_18465 AFA91_18470 AFA91_32665
MHADB586_09890 B586_09895
MDXBTO20_06565 BTO20_13085 BTO20_13090
MSHGMSG_01053(kasB_1) MSG_03170(kasA) MSG_03171(kasB_2)
MFJMFLOJ_00450(kasB_1) MFLOJ_00460(kasA) MFLOJ_23320(kasB_2)
MSTOMSTO_01110(kasB_1) MSTO_01120(kasA) MSTO_27280(kasB_2)
MSIMMSIM_14500(kasB_1) MSIM_45150(kasB_2) MSIM_45160(kasA)
MSAKMSAS_12200(kasA) MSAS_12210(kasB_1) MSAS_12510(kasB_2)
MKUI2456_16055 I2456_16060 I2456_16415
MGROFZ046_00350 FZ046_10765 FZ046_22645 FZ046_22650
MXEMYXE_20720(kasB) MYXE_20730(kasA) MYXE_43070
MNVMNVI_22730 MNVI_45150(kasA) MNVI_45160(kasB)
MPAGC0J29_07930 C0J29_18640 C0J29_18645
MNMMNVM_02370(kasA) MNVM_02380(kasB) MNVM_40280
MGORH0P51_17360 H0P51_17365 H0P51_25650
MCOOMCOO_12820 MCOO_12830(kasB_1) MCOO_39080(kasB_2)
MBAIMB901379_03010(kasA_1) MB901379_03011(kasB)
MSEOMSEO_14160(kasB_1) MSEO_19570(kasA) MSEO_19580(kasB_2)
MPSEMPSD_34150(kasA) MPSD_34160(kasB)
MSHOMSHO_47500(kasA) MSHO_47510(kasB)
MHEKJMUB5695_02081(kasB) JMUB5695_02082(kasA) JMUB5695_04192
MGAUMGALJ_21310 MGALJ_21320 MGALJ_52620
MLJMLAC_22590(kasA) MLAC_22600(kasB)
MBRDMBRA_10910 MBRA_35610(kasB) MBRA_35620(kasA)
MSHJMSHI_10160(kasB) MSHI_10170(kasA)
MKRMKOR_04700(kasB) MKOR_04710(kasA)
MDFK0O62_18260 K0O62_18265
MMAMK3U93_14875 K3U93_14880
MHOLK3U96_10645 K3U96_10650 K3U96_22490
MHERK3U94_09075 K3U94_09080 K3U94_17975
MSENK3U95_05495 K3U95_15995 K3U95_16000
MPAEK0O64_04540 K0O64_18625 K0O64_18630
MSPGF6B93_13965 F6B93_13970
MOTLTS72_07815 LTS72_07820
MLMMLPF_0930(kasA) MLPF_0931(kasB)
MSMMSMEG_1204 MSMEG_4327 MSMEG_4328
MSGMSMEI_1170(kasB) MSMEI_4227(kasA) MSMEI_4228(kasB)
MSBLJ00_05995 LJ00_21445 LJ00_21450
MSNLI99_05995 LI99_21450 LI99_21455
MSHLI98_05995 LI98_21455 LI98_21460
MVAMvan_3756 Mvan_3757 Mvan_4950
MGIMflv_1799 Mflv_2776 Mflv_2777
MSPMspyr1_11830 Mspyr1_22160 Mspyr1_22170
MCBMycch_3278 Mycch_3279 Mycch_5138
MNED174_18305 D174_18310
MYNMyAD_17950 MyAD_17955
MFTXA26_37930 XA26_37940 XA26_50520
MPHLMPHLCCUG_01036(kasB_1) MPHLCCUG_03392(kasA) MPHLCCUG_03393(kasB_2)
MVQMYVA_3578 MYVA_3579 MYVA_4769 MYVA_5709
MLLB1R94_04715 B1R94_10585 B1R94_10590
MRHMycrhN_0961 MycrhN_4434 MycrhN_4435
MTHN4412656_01785(kasB) 4412656_01786(kasA_1)
MHASMHAS_00020(kasB_1) MHAS_01448(kasB_2) MHAS_01449(kasA)
MDUMDUV_08130 MDUV_08140 MDUV_44660
MCHTMCHIJ_33630 MCHIJ_33640
MDRMDOR_04350 MDOR_24810 MDOR_24820
MAUUNCTC10437_03513(kasA) NCTC10437_03514(kasB_1) NCTC10437_04668(kasB_2)
MMAGMMAD_09900(kasB_1) MMAD_35130 MMAD_35140 MMAD_44180(kasB_2) MMAD_50950
MMORMMOR_29430 MMOR_29440 MMOR_56170
MFXMFAL_13630 MFAL_13640
MAICMAIC_34210 MAIC_34220 MAIC_46930
MIJMINS_32340 MINS_32350
MALVMALV_02270 MALV_02280
MTYMTOK_56270 MTOK_56280
MPSCMPSYJ_10760 MPSYJ_30180 MPSYJ_30190
MARZMARA_52190 MARA_61340 MARA_61350
MGADMGAD_02940 MGAD_02950 MGAD_51510
MHEVMHEL_60250 MHEL_60260
MSARMSAR_35160 MSAR_35170
MANYMANY_19070 MANY_19080 MANY_48670
MAUBMAUB_50710 MAUB_50720
MPOFMPOR_39290 MPOR_39300 MPOR_50690(kasB)
MPHUMPHO_18690 MPHO_18700
MMUCC1S78_009090 C1S78_009095
MMATMMAGJ_40560 MMAGJ_73560 MMAGJ_73570
MBOKMBOE_35990 MBOE_36000
MFGK6L26_13125 K6L26_13130 K6L26_26205
MSEIMSEDJ_47240 MSEDJ_47250 MSEDJ_57810
MFLVNCTC10271_01898(kasB_2) NCTC10271_01899(kasA) NCTC10271_04318(kasB_3)
MABMAB_1877c MAB_4608
MMVMYCMA_0969 MYCMA_2544
MABBMASS_1864 MASS_4635
MABLMMASJCM_0440 MMASJCM_1795 MMASJCM_4664
MCHEBB28_09685 BB28_23520
MIZBAB75_10110 BAB75_25995
MSTEMSTE_01795 MSTE_04780
MSAOMYCSP_08125 MYCSP_21495
MSALDSM43276_01633(kasA_1) DSM43276_04441(kasA_2)
MJDJDM601_1649(kasB) JDM601_1650(kasA) JDM601_2027(kasB)
MTER4434518_01562(kasB_1) 4434518_01563(kasA) 4434518_01976(kasB_2)
MMINMMIN_32490(kasB) MMIN_32500(kasA)
MHIBMHIB_10830(kasB) MHIB_10840(kasA) MHIB_26020
ASDAS9A_1223(fabF)
MPAKMIU77_06595(kasB) MIU77_06600(kasA)
NFANFA_16170(kasA)
NFRERS450000_02543(kasA_1)
NCYNOCYR_1672(fabF) NOCYR_5164(kasA)
NBRO3I_001035 O3I_001530 O3I_010905 O3I_039885
NNONONO_c06540(kasA1) NONO_c13390(kasA2) NONO_c20820(kasA3) NONO_c39590(kasA4) NONO_c40520(kasA5)
NSLBOX37_05410 BOX37_07850 BOX37_30445
NSRNS506_02999 NS506_04587 NS506_06033
NTPCRH09_07575 CRH09_11630 CRH09_18150
NOZDMB37_18610 DMB37_22095 DMB37_22610 DMB37_31465
NODFOH10_06500 FOH10_13310 FOH10_19595 FOH10_24550
NAHF5544_08130 F5544_14420 F5544_42295
NADNCTC11293_00291(kasA_1) NCTC11293_02031(kasA_2) NCTC11293_02578(kasA_4)
NWLNWFMUON74_33840 NWFMUON74_34890 NWFMUON74_51900 NWFMUON74_58450
NIEKV110_01045 KV110_10935 KV110_36485 KV110_38945
NTCKHQ06_15840 KHQ06_23050 KHQ06_31165 KHQ06_37615
NHUH0264_15065 H0264_21500 H0264_22775 H0264_30405 H0264_36750
NYALTV02_03220 LTV02_10585 LTV02_18070 LTV02_36510
NGPLTT66_00620 LTT66_03330 LTT66_09725 LTT66_13895
RHARHA1_ro01201(fabF)
RERRER_36740(fabF)
REYO5Y_16895
REBXU06_17105
RQIC1M55_18105
ROPROP_09240(fabF) ROP_12330(fabF)
ROAPd630_LPD05302 Pd630_LPD05661
REQREQ_28970(kasA)
RPYY013_06230
RBYCEJ39_17015
RHBNY08_584
RAVAAT18_15865
RFAA3L23_03833(kasA)
RHSA3Q41_04409(kasA)
RHWBFN03_10195
RRZCS378_05515
RHUA3Q40_01110(kasA)
RHQIM25_20245
RHODAOT96_28425 AOT96_30850
RRT4535765_03064(fabF_2)
RCRNCTC10994_04197(fabF_3)
RTMG4H71_01320
RKOJWS14_06230
RGOKYT97_25490
GBRGbro_3123
GPOGPOL_c28870(kasA)
GORKTR9_3116
GOQACH46_13020
GTABCM27_16010
GITC6V83_13400
GRUGCWB2_16225(kasA)
GOMD7316_00150(kasA)
GAVC5O27_11985
GODGKZ92_14975
GAMGII34_14920
GPDGII33_14045
GJIH1R19_15115
GOILK459_18890
SPINKV203_06400
TPRTpau_2468 Tpau_2726
TSMASU32_13575 ASU32_15195
SRTSrot_0300 Srot_2738
TOYFO059_12230
 » show all
Reference
1  [PMID:11600501]
  Authors
Schaeffer ML, Agnihotri G, Volker C, Kallender H, Brennan PJ, Lonsdale JT
  Title
Purification and biochemical characterization of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthases KasA and KasB.
  Journal
J Biol Chem 276:47029-37 (2001)
DOI:10.1074/jbc.M108903200
  Sequence
[mtu:Rv2245]
Reference
2  [PMID:16267284]
  Authors
Bhatt A, Kremer L, Dai AZ, Sacchettini JC, Jacobs WR Jr
  Title
Conditional depletion of KasA, a key enzyme of mycolic acid biosynthesis, leads to mycobacterial cell lysis.
  Journal
J Bacteriol 187:7596-606 (2005)
DOI:10.1128/JB.187.22.7596-7606.2005
Reference
3  [PMID:19604480]
  Authors
Luckner SR, Machutta CA, Tonge PJ, Kisker C
  Title
Crystal structures of Mycobacterium tuberculosis KasA show mode of action within cell wall biosynthesis and its inhibition by thiolactomycin.
  Journal
Structure 17:1004-13 (2009)
DOI:10.1016/j.str.2009.04.012
  Sequence
[mtu:Rv2245]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.293
IUBMB Enzyme Nomenclature: 2.3.1.293
ExPASy - ENZYME nomenclature database: 2.3.1.293
BRENDA, the Enzyme Database: 2.3.1.293
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