KEGG   ENZYME: 2.4.1.174
Entry
EC 2.4.1.174                Enzyme                                 

Name
glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase;
N-acetylgalactosaminyltransferase I;
glucuronylgalactosylproteoglycan beta-1,4-N-acetylgalactosaminyltransferase;
uridine diphosphoacetylgalactosamine-chondroitin acetylgalactosaminyltransferase I;
UDP-N-acetyl-D-galactosamine:D-glucuronyl-1,3-beta-D-galactosyl-proteoglycan beta-1,4-N-acetylgalactosaminyltransferase;
UDP-N-acetyl-D-galactosamine:D-glucuronyl-(1->3)-beta-D-galactosyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-N-acetyl-D-galactosamine:[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine 4-beta-N-acetylgalactosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [RN:R05929]
Reaction(KEGG)
R05929(G)
Substrate
UDP-N-acetyl-alpha-D-galactosamine [CPD:C00203];
[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [CPD:C04903]
Product
UDP [CPD:C00015];
[protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Comment
Requires Mn2+. Involved in the biosynthesis of chondroitin sulfate. Key enzyme activity for the initiation of chondroitin and dermatan sulfates, transferring GalNAc to the GlcA-Gal-Gal-Xyl-Ser core.
History
EC 2.4.1.174 created 1989, modified 2002
Pathway
ec00532  Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
ec01100  Metabolic pathways
Orthology
K00746  chondroitin sulfate N-acetylgalactosaminyltransferase 1/2
Genes
HSA: 55454(CSGALNACT2) 55790(CSGALNACT1)
PTR: 450416(CSGALNACT2) 464030(CSGALNACT1)
PPS: 100987599(CSGALNACT1) 100994377(CSGALNACT2)
GGO: 101151962(CSGALNACT2) 101154228(CSGALNACT1)
PON: 100172853(CSGALNACT2) 100442293(CSGALNACT1)
NLE: 100583001(CSGALNACT1) 100602432(CSGALNACT2)
MCC: 703703(CSGALNACT2) 704901(CSGALNACT1)
MCF: 102125913(CSGALNACT1) 102136560(CSGALNACT2)
CSAB: 103215414(CSGALNACT1) 103215756(CSGALNACT2)
RRO: 104653929(CSGALNACT2) 104662459(CSGALNACT1)
RBB: 108513317(CSGALNACT2) 108526479(CSGALNACT1)
CJC: 100399122(CSGALNACT2) 100407007(CSGALNACT1)
SBQ: 101027448(CSGALNACT2) 101048885(CSGALNACT1)
MMU: 234356(Csgalnact1) 78752(Csgalnact2)
MCAL: 110295750(Csgalnact2) 110300279(Csgalnact1)
MPAH: 110315909(Csgalnact2) 110336490(Csgalnact1)
RNO: 297554(Csgalnact2) 306375(Csgalnact1)
CGE: 100754969(Csgalnact1) 100770830(Csgalnact2)
NGI: 103733802(Csgalnact2) 103747604(Csgalnact1)
HGL: 101700905(Csgalnact1) 101719048(Csgalnact2)
CCAN: 109688882(Csgalnact1) 109698852(Csgalnact2)
OCU: 100339411(CSGALNACT1) 100339435(CSGALNACT2)
TUP: 102468989(CSGALNACT2) 102490797(CSGALNACT1)
CFA: 482831(CSGALNACT1) 486786(CSGALNACT2)
VVP: 112913575(CSGALNACT2) 112916171(CSGALNACT1)
AML: 100468746(CSGALNACT1) 100484755(CSGALNACT2)
UMR: 103667936(CSGALNACT2) 103677036(CSGALNACT1)
UAH: 113243134(CSGALNACT2) 113245577(CSGALNACT1)
ORO: 101369554(CSGALNACT2) 101377082(CSGALNACT1)
FCA: 101096311(CSGALNACT1) 101100841(CSGALNACT2)
PTG: 102956824(CSGALNACT1) 102966347(CSGALNACT2)
PPAD: 109262244(CSGALNACT1) 109264614(CSGALNACT2)
AJU: 106972130(CSGALNACT2) 106978257(CSGALNACT1)
BTA: 509328(CSGALNACT2) 528057(CSGALNACT1)
BOM: 102266543(CSGALNACT1) 102285203(CSGALNACT2)
BIU: 109553356(CSGALNACT1) 109553754(CSGALNACT2)
BBUB: 102402654(CSGALNACT2) 102406891(CSGALNACT1)
CHX: 102179349(CSGALNACT1) 102185714(CSGALNACT2)
OAS: 101118243(CSGALNACT2) 101120469(CSGALNACT1)
SSC: 100157878(CSGALNACT2) 100517735 102166124(CSGALNACT1)
CFR: 102513810(CSGALNACT1) 102514190(CSGALNACT2)
CDK: 105086444(CSGALNACT1) 105092509(CSGALNACT2)
BACU: 103007226(CSGALNACT2) 103010484(CSGALNACT1)
LVE: 103069443(CSGALNACT2) 103084873(CSGALNACT1)
OOR: 101288535(CSGALNACT2) 101289782(CSGALNACT1)
DLE: 111163996(CSGALNACT2) 111173568(CSGALNACT1)
PCAD: 102982515(CSGALNACT1) 102984892(CSGALNACT2)
ECB: 100050200(CSGALNACT2) 100052192(CSGALNACT1)
EPZ: 103550620(CSGALNACT2)
EAI: 106830921 106831340(CSGALNACT2)
MYB: 102244364(CSGALNACT1) 102262652(CSGALNACT2)
MYD: 102764495(CSGALNACT2) 102768920(CSGALNACT1)
MNA: 107523899(CSGALNACT1) 107532565(CSGALNACT2)
HAI: 109386291(CSGALNACT1) 109395550(CSGALNACT2)
DRO: 112305453(CSGALNACT2) 112321495(CSGALNACT1)
PALE: 102878385(CSGALNACT2) 102889741(CSGALNACT1)
RAY: 107501339(CSGALNACT1) 107517640(CSGALNACT2)
MJV: 108408000(CSGALNACT2) 108409516
LAV: 100654033(CSGALNACT1) 100661660(CSGALNACT2)
MDO: 100012272(CSGALNACT2) 100030310(CSGALNACT1)
SHR: 100925396(CSGALNACT1) 100925905(CSGALNACT2)
PCW: 110196399(CSGALNACT1) 110221474(CSGALNACT2)
OAA: 100076249(CSGALNACT2) 100078026(CSGALNACT1)
GGA: 422488(CSGALNACT1) 423632(CSGALNACT2)
MGP: 100538576(CSGALNACT1) 100550089(CSGALNACT2)
CJO: 107313214(CSGALNACT1) 107315593(CSGALNACT2)
NMEL: 110397662(CSGALNACT1) 110400505(CSGALNACT2)
APLA: 101796158(CSGALNACT2) 101799131(CSGALNACT1)
ACYG: 106032482(CSGALNACT2) 106035122(CSGALNACT1)
TGU: 100223510(CSGALNACT1) 100231851(CSGALNACT2)
LSR: 110468615(CSGALNACT2) 110471712(CSGALNACT1)
SCAN: 103814021(CSGALNACT2) 103825992(CSGALNACT1)
GFR: 102033481(CSGALNACT1) 102043050(CSGALNACT2)
FAB: 101812240(CSGALNACT1) 101820745(CSGALNACT2)
PHI: 102113363(CSGALNACT2) 102113419(CSGALNACT1)
PMAJ: 107203353(CSGALNACT1) 107207041(CSGALNACT2)
CCAE: 111931022(CSGALNACT2) 111944213(CSGALNACT1)
CCW: 104690220(CSGALNACT2) 104697139(CSGALNACT1)
ETL: 114062182(CSGALNACT1) 114062797(CSGALNACT2)
FPG: 101910473(CSGALNACT1) 101919107(CSGALNACT2)
FCH: 102049509(CSGALNACT2) 102059429(CSGALNACT1)
CLV: 102088612(CSGALNACT1) 102097544(CSGALNACT2)
EGZ: 104129088(CSGALNACT2) 104135540(CSGALNACT1)
NNI: 104015153(CSGALNACT2) 104021307(CSGALNACT1)
ACUN: 113479811(CSGALNACT1) 113483540(CSGALNACT2)
PADL: 103924202(CSGALNACT2) 103925418(CSGALNACT1)
AAM: 106484360(CSGALNACT1) 106498950(CSGALNACT2)
ASN: 102388579(CSGALNACT2) 102388616(CSGALNACT1)
AMJ: 102562660(CSGALNACT2) 106736723(CSGALNACT1)
PSS: 102447091(CSGALNACT1) 102458703(CSGALNACT2)
CMY: 102940405(CSGALNACT2) 102945055(CSGALNACT1)
CPIC: 101937753(CSGALNACT2) 101950451(CSGALNACT1)
ACS: 100558813(csgalnact2) 100560783(csgalnact1)
PVT: 110083670(CSGALNACT2) 110086324(CSGALNACT1)
PBI: 103048163(CSGALNACT1) 103058428(CSGALNACT2)
PMUR: 107293256(CSGALNACT2) 107302358(CSGALNACT1)
TSR: 106549275(CSGALNACT1) 106551544(CSGALNACT2)
PMUA: 114581524(CSGALNACT1) 114597096(CSGALNACT2)
GJA: 107107034(CSGALNACT1) 107115005(CSGALNACT2)
XLA: 108696289(csgalnact2.L) 108706822(csgalnact1.S) 108712229(csgalnact1.L)
XTR: 100124741(csgalnact2) 100497098(csgalnact1)
NPR: 108786604(CSGALNACT2) 108804915(CSGALNACT1)
DRE: 101886904(csgalnact1b) 560826(csgalnact2) 793671(csgalnact1a)
SRX: 107712620 107713273 107726950(csgalnact2) 107743585 107744099(csgalnact1)
IPU: 108263569(csgalnact2) 108265223 108277248(csgalnact1)
PHYP: 113533267 113541925(csgalnact2) 113543992
AMEX: 103021869 103029300(csgalnact2) 103045746
EEE: 113585223(csgalnact2) 113590383
TRU: 101071007 101079052(csgalnact2) 101079135
LCO: 104920113(csgalnact1) 104920841 104921883(csgalnact2)
NCC: 104951713 104952731(csgalnact2)
MZE: 101469922(csgalnact1) 101474654 101476345(csgalnact2)
ONL: 100702075(csgalnact2) 100706120 100708917
OLA: 101155793(csgalnact2) 101159591 101162617(csgalnact1)
XMA: 102221723(csgalnact1) 102233977 102237743(csgalnact2)
XCO: 114137572(csgalnact2) 114149526(csgalnact1) 114154568
PRET: 103470100 103473476(csgalnact1) 103477114(csgalnact2)
CVG: 107086272 107088483(csgalnact2) 107088980
NFU: 107373900(csgalnact1) 107375371(csgalnact2) 107394138
KMR: 108231091(csgalnact1) 108233606 108240679(csgalnact2)
AOCE: 111564714 111564820(csgalnact1) 111573086(csgalnact2)
CSEM: 103387459(csgalnact2) 103390413(csgalnact1) 103397716
POV: 109626572 109632851(csgalnact2) 109637154
LCF: 108874113 108882236 108886250(csgalnact2)
SDU: 111220601(csgalnact2) 111230915 111239886(csgalnact1)
SLAL: 111645131 111656793(csgalnact1) 111669464
HCQ: 109523133(csgalnact2) 109525674 109527907
BPEC: 110157439 110162232(csgalnact2) 110167048
MALB: 109956612 109960538(csgalnact2) 109972367
ELS: 105014092 105026679(csgalnact1) 105028646(csgalnact2)
SFM: 108924956(csgalnact1) 108932703(csgalnact2)
PKI: 111849050(csgalnact2) 111849915(csgalnact1)
LCM: 102358556(CSGALNACT2) 102366614(CSGALNACT1)
CMK: 103181211(csgalnact2) 103186731(csgalnact1)
RTP: 109912510(csgalnact2) 109917511(csgalnact1)
BFO: 118410920
CIN: 100177589
SPU: 579124
APLC: 110991016
SKO: 100368195
DME: Dmel_CG12913(CG12913)
DER: 6543177
DSE: 6608480
DSI: Dsimw501_GD10724(Dsim_GD10724)
DAN: 6495632
DSR: 110178256
DPE: 6590525
DMN: 108159923
DWI: 6646033
DAZ: 108615801
DNV: 108653728
DVI: 6625749
AAG: 5578232
NVI: 100121958
CSOL: 105364133
MDL: 103578840
TCA: 659298
DPA: 109542837
ATD: 109600607
NVL: 108559669
BTAB: 109034694
CLEC: 112126951
ZNE: 110838755
PTEP: 107456076
PCAN: 112575074
CRG: 105325571
MYI: 110456749
LAK: 106167613
DGT: 114517412
AQU: 105313529
 » show all
Reference
1  [PMID:3922754]
  Authors
Rohrmann K, Niemann R, Buddecke E.
  Title
Two N-acetylgalactosaminyltransferase are involved in the biosynthesis of chondroitin sulfate.
  Journal
Eur J Biochem 148:463-9 (1985)
DOI:10.1111/j.1432-1033.1985.tb08862.x
Reference
2  [PMID:11788602]
  Authors
Uyama T, Kitagawa H, Tamura Ji J, Sugahara K.
  Title
Molecular cloning and expression of human chondroitin N-acetylgalactosaminyltransferase: the key enzyme for chain initiation and elongation of chondroitin/dermatan sulfate on the protein linkage region tetrasaccharide shared by heparin/heparan sulfate.
  Journal
J Biol Chem 277:8841-6 (2002)
DOI:10.1074/jbc.M111434200
  Sequence
[hsa:55790]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.174
IUBMB Enzyme Nomenclature: 2.4.1.174
ExPASy - ENZYME nomenclature database: 2.4.1.174
BRENDA, the Enzyme Database: 2.4.1.174
CAS: 96189-39-8
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